[1]金 超,王 婷.蒙古族急性肺血栓栓塞症患者临床特征分析及其与蛋白质组学的关系研究[J].现代检验医学杂志,2026,41(02):29-36.[doi:10.3969/j.issn.1671-7414.2026.02.006]
　JIN Chao,WANG Ting.Clinical Characteristics and Its Relationship with Proteomics in Mongolian Patients with Acute Pulmonary Thromboembolism[J].Journal of Modern Laboratory Medicine,2026,41(02):29-36.[doi:10.3969/j.issn.1671-7414.2026.02.006]
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蒙古族急性肺血栓栓塞症患者临床特征分析及其与蛋白质组学的关系研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年02期
页码:
29-36
栏目:
论著
出版日期:
2026-03-15

文章信息/Info

Title:
Clinical Characteristics and Its Relationship with Proteomics in Mongolian Patients with Acute Pulmonary Thromboembolism
文章编号:
1671-7414（2026）02-029-08
作者:
金 超，王 婷
内蒙古自治区人民医院心血管内科，呼和浩特 010010
Author(s):
JIN Chao，WANG Ting
Department of Cardiovascular Medicine, Inner Mongolia Autonomous Region People’s Hospital, Hohhot 010010，China
关键词:
蒙古族; 急性肺血栓栓塞症; 临床特征分析; 蛋白质组学
分类号:
R563.5；R446.11
DOI:
10.3969/j.issn.1671-7414.2026.02.006
文献标志码:
A
摘要:
目的分析蒙古族急性肺血栓栓塞症(APTE)患者临床特征,并探究其与血清蛋白质组学的关系。方法选择2022年1月~2025年1月在内蒙古自治区人民医院就诊的413例蒙古族APTE患者作为研究对象,并根据APTE严重程度分为:低危组(n=66)、中危组(n=319)和高危组(n=28)。分析三组患者基线资料、血清生化指标方面存在的差异。选择180例同期体检的健康者纳入对照组,分析APTE患者与同期体检健康者在血清生化指标方面的差异。蛋白质标记定量技术结合液相色谱-串联质谱联用(LC-MS/MS)技术鉴定和筛选APTE的差异蛋白,并分析差异蛋白的生物学功能及富集的信号通路。结果APTE不同危险度患者在高血压、静脉血栓栓塞、慢性阻塞性肺疾病(COPD)方面的差异具有统计学意义(χ2=6.654、7.267、35.251,均P<0.05)。随着APTE危险度增加,纤维蛋白原(FIB)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白-胆固醇(LDL-C)、C-反应蛋白(CRP)、D-二聚体(D-D)、肌钙蛋白I(cTnI)和N-末端脑利钠肽前体(NT-proBNP)明显上升(F/H=3.669~786.487),白蛋白(ALB)、高密度脂蛋白-胆固醇(HDL-C)明显下降(F=19.143、36.954),差异具有统计学意义(均P<0.05)。各危险度APTE患者白细胞(WBC)、红细胞体积分布宽度(RDW)、FIB、TC、TG、LDL-C、CRP、D-D、cTnI和NT-proBNP明显高于对照组(F/H=16.859~1740.668),血小板(PLT)、血红蛋白(Hb)、ALB和HDL-C明显低于对照组(F=37.228~123.817),差异具有统计学意义(均P<0.05)。APTE患者与对照组血清蛋白质组学共筛查鉴定出352个差异蛋白。与对照组比较,研究组患者4个蛋白显著上调,10个蛋白显著下调。差异蛋白主要参与生物学过程调控、代谢过程和跨膜运输等生物学过程,构成细胞质、细胞器等细胞成分,发挥蛋白质结合、酶催化活性、酶水解活性与能量运输活性等分子功能;主要富集于信号分子和相互作用、碳水化合物代谢和脂质代谢等信号通路。3个上调蛋白均与WBC、ALB、FIB、CRP、D-D、cTnI、NT-proBNP显著相关(r=-0.73~0.92,均P<0.05),且上调蛋白载脂蛋白C-Ⅲ还与ALB、FIB、TC、TG、HDL-C、LDL-C、CRP、D-D、cTnI、NT-proBNP显著相关(r=-0.74~0.78,均P<0.05);9个下调蛋白均与FIB、CRP、D-D、NT-proBNP显著相关(r=-0.95~-0.72,均P<0.05),且抗凝血酶Ⅲ、凝血因子Ⅻ和前胶原C端肽醇增强子还与PLT呈显著正相关(r=0.56~0.68,均P<0.05)。结论蒙古族APTE患者临床特征多样,与危险度存在一定关联。血清生化指标随危险度增加而变化。APTE相关差异蛋白主要参与信号分子和相互作用、碳水化合物代谢和脂质代谢等调控过程,且同临床指标密切相关,为蒙古族APTE的发病机制和诊断提供了潜在生物标志物。
Abstract:
Objective To analyze the clinical characteristics of Mongolian patients with acute pulmonary thromboembolism (APTE) and explore its relationship with proteomics. Methods 413 Mongolian APTE patients who received treatment at Inner Mongolia Autonomous Region people’s Hospital from January 2022 to January 2025 were selected as the research subjects and grouped according to the severity of APTE into low-risk group (n=66), medium-risk group (n=319) and high-risk group (n=28). The differences in baseline characteristics, and serum biochemical indexes among the three groups were analyzed. A total of 180 healthy subjects undergoing physical examinations at the same time were included in the healthy group. The differences in serum biochemical indexes between APTE patients and healthy subjects were analyzed. The differential proteins in APTE were identified and screened by quantitative protein profiling combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The biological functions and enriched signaling pathways of these differential proteins were further analyzed.Results There were statistically significant differences in hypertension, venous thromboembolism and chronic obstructive pul-monary disease among patients with different risk of APTE (χ2=6.654, 7.267, 35.251, all P <0.05). As the risk of APTE increased, fibrinogen (FIB), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), C-reactive protein (CRP), D-dimer (D-D), cardiac troponin I (cTnI) and N-terminal probrain natriuretic peptide (NT-proBNP) were significantly in-creased (F/H=3.669~786.487), Albumin (ALB) and high-density lipoprotein cholesterol (HDL-C), were significantly decreased (F=19.143, 36.954), and the differences were statisticany significant (all P<0.05). White blood cells (WBC), red blood cell distri-bution width (RDW), FIB, TC, TG, LDL-C, CRP, D-D, cTnI and NT-proBNP in APTE patients with different risk levels were significantly higher than those in healthy subjects (F/H=16.859~1 740.668, all P<0.05). Platelet count (PLT), hemoglobin (Hb), ALB and HDL-C were significantly lower than those of healthy subjects (F=37.228~123.817), and the differences were statisti-cally significant (all P<0.05). A total of 352 differentially expressed proteins were identified by serum proteomics screening be-tween APTE patients and healthy subjects. Compared with healthy subjects, 4 proteins were significantly up-regulated and 10 proteins were significantly down-regulated in APTE patients. The bioinformatics analysis showed that these differentially ex-pressed proteins were mainly involved in biological processes such as regulation of biological processes, metabolic processes and transmembrane transport, forming cellular components such as cytoplasm and organelles, and exerting molecular functions such as protein binding, enzymatic catalytic activity, enzymatic hydrolysis activity and energy transport activity. The differentially ex-pressed proteins primarily participated in signaling pathways such as signaling molecules and interactions, carbohydrate metabo-lism and lipid metabolism. The 3 up-regulated proteins were significantly associated with WBC, ALB, FIB, CRP, D-D, cTnI and NT-proBNP (r=-0.73~0.92, all P<0.05). Additionally, the up-regulated protein apolipoprotein C-Ⅲ showed significant correla-tions with ALB, FIB, TC, TG, HDL-C, LDL-C, CRP, D-D, cTnI and NT-proDNP (r=-0.74~0.78, all P<0.05). The 9 down-regu-lated proteins were significantly assciated with FIB, CRP, D-D and NT-proBNP (r=-0.95~-0.72, all P <0.05). Furthermore, anti-thrombin-Ⅲ, coagulation factor Ⅻ and procollagen C-endopeptidase enhancer exhibited significant correlations with PLT(r=0.56~0.68, all P<0.05). Conclusions The clinical characteristics of Mongolian APTE patients are diverse, and there is a certain correlation with the risk levels of APTE. Serum biochemical parameters change with the increase of risk levels. APTE-as-sociated differentially expressed proteins are mainly involved in the regulatory processes of signaling molecules and interactions, carbohydrate metabolism and lipid metabolism, and are closely related to clinical indexes, which provide potential biomarkers for elucidating the pathogenesis and aiding diagnosis of APTE in the Mongolian population.

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备注/Memo

备注/Memo:
基金项目：内蒙古自治区人民医院院内基金（编号：2022YN29）。
作者简介：金超（1989-），男，硕士，副主任医师，研究方向：肺动脉高压，E-mail：1140238678@qq.com。
通讯作者：王婷（1988-），女，硕士，副主任医师，研究方向：肺动脉高压，E-mail：85457344@qq.com。
更新日期/Last Update: 2026-03-15