[1]李珺玲,袁 莉,吴文婧,等.dRVVT标准化比值和Rosner指数在多点稀释法测定狼疮抗凝物阳性血浆内源性凝血因子活性中的应用研究[J].现代检验医学杂志,2026,41(03):34-37+45.[doi:10.3969/j.issn.1671-7414.2026.03.006]
　LI Junling,YUAN Li,WU Wenjing,et al.Study on the Application of dRVVT Normalized Ratio and Rosner Index in Assessing the Activity of Endogenous Coagulation Factors in Lupus Anticoagulant-Positive Plasma Using Multi Dilution Analysis[J].Journal of Modern Laboratory Medicine,2026,41(03):34-37+45.[doi:10.3969/j.issn.1671-7414.2026.03.006]
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dRVVT标准化比值和Rosner指数在多点稀释法测定狼疮抗凝物阳性血浆内源性凝血因子活性中的应用研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年03期
页码:
34-37+45
栏目:
论著
出版日期:
2026-05-13

文章信息/Info

Title:
Study on the Application of dRVVT Normalized Ratio and Rosner Index in Assessing the Activity of Endogenous Coagulation Factors in Lupus Anticoagulant-Positive Plasma Using Multi Dilution Analysis
文章编号:
1671-7414（2026）03-034-05
作者:
李珺玲，袁 莉，吴文婧，刘 妮
西安交通大学第一附属医院检验科，西安 710061
Author(s):
LI Junling，YUAN Li，WU Wenjing，LIU Ni
Department of Clinical Laboratory, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
关键词:
狼疮抗凝物; 内源性凝血因子; 稀释蝰蛇毒时间试验标准化比值; Rosner指数; 多点稀释分析
分类号:
R593.24；R446.11
DOI:
10.3969/j.issn.1671-7414.2026.03.006
文献标志码:
A
摘要:
目的?探讨稀释蝰蛇毒时间试验（dRVVT）标准化比值和Rosner指数在多点稀释分析（MDA）解决狼疮抗凝物（LA）阳性血浆内源性凝血因子检测最佳稀释比例中的指导作用。方法?选择2024年5～8月于西安交通大学第一附属医院就诊患者中dRVVT提示LA阳性标本70例，并根据dRVVT标准化比值分为三组：低值组（1.2～1.5）、中值组（1.5～2.0）和高值组（≥2.0），多点稀释测定内源性凝血因子活性，分析不同稀释度的因子活性结果；同时对其中25例进行活化部分凝血活酶时间（APTT）即刻纠正试验，分析Rosner指数与最佳稀释比例的相关性。结果dRVVT标准化比值低值组可选择原倍和1∶22两个稀释度；中值组可选择1∶22和1∶23两个稀释度；而高值组至少以1∶23作为初始稀释度。Rosner指数与dRVVT标准化比值及最佳稀释比例之间无明显相关性（r=-0.095、0.197，P=0.651、0.346）。结论dRVVT标准化比值可用于指导LA阳性血浆内源性凝血因子检测的最佳稀释比例，Rosner指数与最佳稀释比例之间无明显相关性。
Abstract:
Objective To explore the role of the dilute Russell viper venom time (dRVVT) normalized ratio and the Rosner index in determining the optimal dilution ratio for detecting endogenous coagulation factor activity in lupus anticoagulant (LA)-positive plasma through multi dilution analysis (MDA). Methods 70 specimens from patients at the First Affiliated Hospital of Xi’an Jiaotong Univer-sity from May 2024 to August 2024 that tested positive for lupus anticoagulant (LA) via dRVVT were selected and divided into three groups based on the dRVVT normalized ratio: the low-value group (1.2~1.5), the medium-value group (1.5~2.0), and the high-value group (≥2.0). The activity of endogenous coagulation factors was measured using MDA, and the results of factor activity at different dilutions were analyzed. Concurrently, the immediate correction test for activated partial thromboplastin time (APTT) was performed on 25 of these cases to analyze the correlation between the Rosner index and the optimal dilution ratio. Results For the low-value group, both the undiluted sample and a 1:22 dilution could be selected. For the median value group, a dilution of 1:22 or 1:23 could be selected. For the high-value group, a 1:23 dilution was required as the initial dilution. No significant correlation was found between the Rosner index and the dRVVT normalized ratio or the optimal dilution ratio (r=-0.095, P=0.651; r=0.197, P=0.346). Conclusions The dRVVT normalized ratio can guide the selection of the optimal dilution ratio for the detection of endogenous coagulation factors in LA-positive plasma, and there is no significant correlation between the Rosner index and the optimal dilution ratio.

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备注/Memo

备注/Memo:
基金项目：陕西省自然科学基础研究计划（2023-JC-YB-666）。
作者简介：李珺玲（1998-），女，硕士，初级检验师，研究方向：临床出凝血检验，E-mail：ljl9814@163.com。
通讯作者：袁莉（1982-），女，博士在读，副主任技师，研究方向：临床出凝血检验，E-mail：yuanli2006sky@126.com。
更新日期/Last Update: 2026-05-15