[1]华佳敏,钱向雨,王小钦,等.慢性淋巴细胞白血病骨髓细胞遗传学检测中添加脂多糖提高核型分析效能的应用研究[J].现代检验医学杂志,2026,41(03):46-49.[doi:10.3969/j.issn.1671-7414.2026.03.008]
　HUA Jiamin,QIAN Xiangyu,WANG Xiaoqin,et al.Application Study on the Addition of Lipopolysaccharide to Bone Marrow Cytogenetic Detection in Chronic Lymphocytic Leukemia to Enhance the Efficacy of Karyotype Analysis[J].Journal of Modern Laboratory Medicine,2026,41(03):46-49.[doi:10.3969/j.issn.1671-7414.2026.03.008]
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慢性淋巴细胞白血病骨髓细胞遗传学检测中添加脂多糖提高核型分析效能的应用研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年03期
页码:
46-49
栏目:
论著
出版日期:
2026-05-13

文章信息/Info

Title:
Application Study on the Addition of Lipopolysaccharide to Bone Marrow Cytogenetic Detection in Chronic Lymphocytic Leukemia to Enhance the Efficacy of Karyotype Analysis
文章编号:
1671-7414（2026）03-046-04
作者:
华佳敏1，钱向雨1，王小钦2，徐玉兵1
1.上海金域医学检验所有限公司细胞遗传室，上海 201321;2.复旦大学附属华山医院血液科，上海200040
Author(s):
HUA Jiamin1，QIAN Xiangyu1，WANG Xiaoqin2，XU Yubing1
1.Cytogenetics Laboratory, KingMed Diagnostics (Shanghai) Laboratory, Shanghai 201321, China;2.Department of Hematology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China
关键词:
慢性淋巴细胞白血病; 脂多糖; 细胞遗传学; 染色体核型分析
分类号:
R557.4；Q503
DOI:
10.3969/j.issn.1671-7414.2026.03.008
文献标志码:
A
摘要:
目的?研究脂多糖（LPS）在慢性淋巴细胞白血病（CLL）细胞遗传学检测中的应用价值，以期提高CLL的染色体核型分析效能。方法选取2020年6月~2023年12月送检上海金域医学检验所有限公司的93例诊断为CLL的骨髓样本，每例样本同期分别接种2种培养液，对照组培养液中不含LPS，实验组在培养液中添加LPS（83?g/ml），采用G显带技术进行染色体核型分析。比较实验组与对照组的染色体培养成功率、异常核型检出率和复杂核型检出率。结果实验组与对照组染色体培养成功率分别为91.40%、87.10%，复杂核型检出率分别为18.82%、9.88%，差异无统计学意义（χ2=0.90、2.68，均P>0.05）；染色体异常核型检出率分别为56.47%、28.40%，差异具有统计学意义（χ2=13.36，P=0.0003）。5例核型异常样本同步做了荧光原位杂交（FISH）检测，结果表明添加LPS的染色体核型分析异常结果与FISH检测结果一致。结论LPS刺激剂的添加可以提高CLL患者的染色体异常核型检出率，并可检测到更多复杂核型，为CLL的临床诊疗和预后分层提供更多有用的信息，可在CLL的染色体核型分析中推广使用。
Abstract:
Objective To investigate the application value of lipopolysaccharide (LPS) in cytogenetic detection of chronic lympho-cytic leukemia (CLL), and improve the efficiency of chromosome karyotype analysis in CLL. Methods A total of 93 bone marrow samples diagnosed with CLL and submitted to KingMed Diagnostics Shanghai Laboratory from June 2020 to December 2023 were enrolled. Each sample was inoculated into two culture media simultaneously: the control medium without LPS, while the experi-mental medium supplemented with LPS (83 ?g/ml). Chromosome karyotype analysis was performed using G-banding technique. The culture success rate, detection rate of abnormal karyotypes, and detection rate of complex karyotypes were compared between the experimental group and the control group. Results The success rates of chromosome culture in the experimental group and the control group were 91.40% and 87.10%, respectively, and the detection rates of complex karyotypes were 18.82% and 9.88%, respectively, without statistically significant differences (χ2=0.90, 2.68, all P＞0.05). The detection rates of abnormal karyotypes were 56.47% and 28.40%, respectively, with a statistically significant difference (χ2=13.36, P=0.000 3). Five samples with abnormal karyotypes were simultaneously detected by fluorescence in situ hybridization (FISH), and the results showed that the abnormal karyotypes identified by LPS stimulated chromosome analysis were consistent with FISH results. Conclusions Application of LPS can improve the detection rate of abnormal karyotypes for CLL, and help identify more complex karyotypes, providing more useful information for clinical diagnosis, treatment and prognosis stratification of CLL. LPS can be widely used in karyotype analysis for CLL.

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备注/Memo

备注/Memo:
作者简介：华佳敏（1982-），女，本科，主管技师，研究方向：细胞遗传学，E-mail：58680937@qq.com。
通讯作者： 王小钦（1968-），女，博士，主任医师，研究方向：血液肿瘤，E-mail：wangxiaoqin@shmu.edu.cn。
徐玉兵（1984-），男，本科，副主任技师，研究方向：医学检验，E-mail：sh-xuyubing@kingmed.com.cn，同为通讯作者。
更新日期/Last Update: 2026-05-15