[1]孙文利,刘红星,陈佳琦,等.造血干细胞移植患者移植前后血浆中氨基酸、微量元素及重金属、维生素、类固醇激素的含量检测分析[J].现代检验医学杂志,2020,35(03):31-35.[doi:10.3969/j.issn.1671-7414.2020.03.007]
　SUN Wen-li,LIU Hong-xing,CHEN Jia-qi,et al.Detection and Analysis the Content of Amino Acids, Trace Elements and Heavy Metals, Vitamins, and Steroid Hormones in Plasma of Patients with Hematopoietic Stem Cell Transplantation before and after Transplantation[J].Journal of Modern Laboratory Medicine,2020,35(03):31-35.[doi:10.3969/j.issn.1671-7414.2020.03.007]
点击复制

造血干细胞移植患者移植前后血浆中氨基酸、微量元素及重金属、维生素、类固醇激素的含量检测分析()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年03期
页码:
31-35
栏目:
论著
出版日期:
2020-07-20

文章信息/Info

Title:
Detection and Analysis the Content of Amino Acids, Trace Elements and Heavy Metals, Vitamins, and Steroid Hormones in Plasma of Patients with Hematopoietic Stem Cell Transplantation before and after Transplantation
文章编号:
1671-7414（2020）03-031-06
作者:
孙文利; 刘红星; 陈佳琦; 李明花; 王　磊
（河北燕达陆道培医院，河北廊坊　065201）
Author(s):
SUN Wen-li;  LIU Hong-xing;  CHEN Jia-qi;  LI Ming-hua;  WANG Lei
（Hebei Yanda Lu Daopei Hospital, Hebei Langfang 065201, China）
关键词:
造血干细胞移植; 氨基酸; 微量元素及重金属; 维生素; 类固醇激素
分类号:
R457.7；R446.11
DOI:
10.3969/j.issn.1671-7414.2020.03.007
摘要:
目的　了解造血干细胞移植患者移植前后血浆中氨基酸、微量元素及重金属、维生素、类固醇激素水平的变化。 方法　选取44 例在河北燕达陆道培医院治疗的患者作为研究对象，按照回输干细胞的时间分为移植前、移植期和移植 后三组，采用质谱法测定三组患者血浆中氨基酸、微量元素及重金属、维生素、类固醇激素含量。结果　与移植前相比， 移植期患者血浆中天冬氨酸（aspartic acid，Asp）、瓜氨酸（citrulline，Cit）、羟脯氨酸（hydroxyproline，Hyp）、镁（Mg）、 砷（As）、维生素D3，5- 甲基四氢叶酸（5-methyltetrahydrofolic acid，5-MTHF）、可的松、皮质酮和氢化可的松浓度 明显较低，锌（Zn）、铬（Cr）和锶（Sr）浓度偏高，差异均具有统计学意义（均P<0.05）；与移植期比较，移植后 患者体内3- 甲基组氨酸（3-methylhistidine，3MHis）、丙氨酸（alanine，Ala）、Cit，甘氨酸（glycine，Gly）、Hyp， 磷酸乙醇胺（phosphoethanolamine，PetN）、肌氨酸（sarcosine，Sar）、Mg，As，硒（Se）、维生素A、维生素E、 维生素K 和5-MTHF 浓度均显著增高，苯丙氨酸（phenylalanine，Phe）和Cr 浓度减少，差异均具有统计学意义（均 P<0.05）。结论　监测造血干细胞移植患者血浆中氨基酸、微量元素及重金属、维生素、类固醇激素含量变化，对血液 病患者有选择的进行营养补充，提高免疫功能及血液病患者的治疗有一定的意义。
Abstract:
Objective　To investigate the plasma levels of amino acids, trace elements and heavy metals, vitamins, and steroid hormones in patients with hematopoietic stem cell transplantation. Methods　44 Leukemia patients in Hebei Yanda Lu Daopei Hospital was selected in this study that were divided into three groups (pre-transplanted, transplanting, after transplantation) according to before, during, after peripheral stem cells return infusion. The content of amino acids, trace elements and heavy metals, vitamins and steroid hormones in plasma of three groups of patients was determined by mass-spectrography. Results　Compared with before transplantation, the plasma levels of Asp, Cit, Hyp, Mg, As, vitamin D3, 5-MTHF, cortisone, corticosterone and hydrocortisone were significantly lower during transplantation period, and Zn, Cr and Sr were higher, the differences were statistically significant(P<0.05).Compared with transplantation period, the plasma levels of 3MHis, Ala, Cit, Gly, Hyp, PetN, Sar, Mg, As, Se, vitamin A, vitamin E, vitamin K, and 5-MTHF were significantly increased , and Phe and Cr were decreased after transplantation, the differences were statistically significant (P<0.05). Conlusion　Monitoring the plasma levels of amino acids, trace elements and heavy metals, vitamins, and steroid hormones in patients with hematopoietic stem cell transplantation has certain significance for the selective nutritional supplementation of patients with hematological diseases and the improvement of immune function and treatment of patients with hematological diseases.

参考文献/References:

[1]　付海慧. 造血干细胞移植治疗恶性血液病的研究与 分析[J]. 中外医疗, 2018, 37(1): 48-50. 　　 FU Haihui. Research and analysis of hematopoietic stem cell transplantation for hematologic malignancies[ J]. China Foreign Medical Treatment, 2018, 37(1): 48-50.
[2]　RAMACHANDRAN V, KOLLI S S, STROWD L C. Review of graft-versus-host disease[J]. Dermatologic Clinics, 2019, 37(4):569-582.
[3]　L? Meng, HUANG Xiaojun. Allogeneic hematopoietic stem cell transplantation in China: where we are and where to go[J]. Journal of Hematology & Oncology, 2012, 5(1): 10.
[4]　FERREIRA E E, GUERRA D C,BALUZ K, et al. Nutritional status of patients submitted to transplantation of allogeneic hematopoietic stem cells: a retrospective study[J]. Rev Bras HematolHemoter. 2014,36(6):414- 419.
[5]　闫晓红, 孙萱, 张杰. 营养支持对白血病化疗患者 营养状况及不良反应的影响[J]. 中国食物与营养, 2018, 24(9):71-73. 　　 YAN Xiaohong, SUN Xuan, ZHANG Jie. Effects of enteral nutrition support on nutritional status and adverse reactions of patients with leukemia chemotherapy[J]. Food and Nutrition in China, 2018, 24(9):71-73.
[6]　郝素娟, 李惠玲, 景秀琛, 等. 异基因造血干细胞移 植期间患者营养状态及其影响因素的分析[J]. 中国 实用护理杂志, 2015, 31(3):161-165. 　　 HAO Sujuan, LI Huiling, JING Xiuchen, et al. Nutritional status and its related factors in patients undergoing allogenetic hematopoietic stem cell transplantation[J]. Chinese Journal of Practical Nursing, 2015, 31(3):161-165.
[7]　　RAYNARD B, NITENBERG G, GORY-DELABAERE G, et al. Summary of the Standards, Options and Recommendations for nutritional support in patients undergoing bone marrow transplantation (2002)[J]. British Journal of Cancer, 2003, 89(Suppl 1): S101– S106.
[8]　　王新颖. 骨髓移植患者的营养支持治疗[J]. 肿瘤代谢 与营养电子杂志, 2014, 1(2):46-50. 　　 WANG Xinying. Nutrition support therapy for patients with bone marrow transplant[J]. Electronic Journal of Metabolism and Nutrition of Cancer, 2014, 1(2):46-50.
[9]　　张之南, 沈梯. 血液病的诊断及疗效标准[M].3 版. 北 京: 科学出版社,2007:19-65. 　　 ZHANG Zhinan, SHEN Ti. Blood disease diagnosis and curative effect of standard[M].3th Ed. Beijing:Science Press,2007:19-65.
[10] WARBURG O. On the origin of cancer cells[J]. Science, 1956, 123(3191):309-314.
[11] 关阳, 黄雅静, 李惠子, 等. 恶性血液病化疗患者血 浆氨基酸动态监测及临床意义[J]. 营养学报, 2013, 35(6):549-553. 　　 GUAN Yang，HUANG Yajing，LI Huizi， et al. Dynamic monitoring of plasma amino acids in chemotherapy of patients with hematological malignancies and its clinical value[J]. Acta Nutrimenta Sinica, 2013, 35(6):549-553.
[12] WILKINSON A C , MORITA M , NAKAUCHI H , et al. Branched-chain amino acid depletion conditions bone marrow for hematopoietic stem cell transplantation avoiding amino acid imbalance-associated toxicity[J]. Experimental Hematology, 2018,63:12-16e1.
[13] 许子亮, 吴蕴棠, 李睿, 等. 205 例成年血液病患 者的膳食营养调查[J]. 临床血液学杂志, 2011, 24(1):50-53. 　　 XU Ziliang, WU Yuntang, LI Rui, et al. Dietary nutrition survey of 205 cases of adult patients with blood diseases[J]. Journal of Clinical Hematology, 2011, 24(1):50-53.
[14] 李佳丽, 曾东风, 李杰平, 等. 白血病患儿全血微量 元素含量变化及其意义[J]. 中华临床医师杂志( 电 子版), 2013,7(24):11877-11879. 　　 LI Jiali, ZENG Dongfeng, LI Jieping, et al. Changes of trace elements in whole blood of children with leukemia and their significance [J]. Chin J Clinicians(Electronic Edition), 2013, 7(24): 11877-11879.
[15] EBY G A . Treatment of acute lymphocytic leukemia using zinc adjuvant with chemotherapy and radiation - A case history and hypothesis[J]. Medical Hypotheses, 2005, 64(6):1124-1126.
[16] 刘印彩. 白血病患者血清中微量元素锌、铜、硒、 镁水平研究[D]. 石家庄：河北医科大学, 2015. 　　 LIU Yincai. The research about serum zinc, copper, selenium, magnesium of leukemia patient and disease outcome[D]. Shijiaozhuang:Hebei Medical University, 2015.
[17] NANNYA Y, SHINOHARA A, ICHIKAWA M, et al. Serial profile of vitamins and trace elements during the acute phase of allogeneic stem cell transplantation[J]. Biology of Blood and Marrow Transplantation, 2014, 20(3):430-434.
[18] WALLACE G, JODELE S, HOWELL J, et al. Vitamin D deficiency and survival in children after hematopoietic stem cell transplant[J]. Biology of Blood and Marrow Transplantation, 2015, 21(9): 1627-1631.

备注/Memo

备注/Memo:
收稿日期：2019-12-12 修回日期：2020-02-22作者简介：孙文利（1989-），女，硕士，主管药师，主要从事医院药学研究工作，E-mail：sunwenli1215@163.com。 通讯作者：王磊（1984-），男，硕士，助理研究员，主要从事医院药学研究工作。
更新日期/Last Update: 2020-07-20