[1]杜伟平,陈延平,阳　央,等.新型冠状病毒肺炎患者多类型样本SARS-CoV-2 核酸检测在临床诊疗中的意义[J].现代检验医学杂志,2020,35(03):87-89.[doi:10.3969/j.issn.1671-7414.2020.03.022]
　DU Wei-ping,CHEN Yan-ping,YANG Yang,et al.Clinical Significance of Detection of SARS-CoV-2 Nucleic Acid in Patients with COVID-19[J].Journal of Modern Laboratory Medicine,2020,35(03):87-89.[doi:10.3969/j.issn.1671-7414.2020.03.022]
点击复制

新型冠状病毒肺炎患者多类型样本SARS-CoV-2 核酸检测在临床诊疗中的意义()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年03期
页码:
87-89
栏目:
新型冠状病毒肺炎检验专题
出版日期:
2020-07-20

文章信息/Info

Title:
Clinical Significance of Detection of SARS-CoV-2 Nucleic Acid in Patients with COVID-19
文章编号:
1671-7414（2020）03-087-03
作者:
杜伟平1; 陈延平1; 阳　央2; 冯　智3; 王　东3; 刘　蓉3; 侯　欢3; 安　旭3; 李芳芹1
（1. 延安大学附属医院，陕西延安 716000；2. 延安大学医学院，陕西延安 716000；3. 延安市第二人民医院， 陕西 延安716000）
Author(s):
DU Wei-ping1;  CHEN Yan-ping1;  YANG Yang2;  FENG Zhi3;  WANG Dong3;  LIU Rong3;  HOU Huan3;  AN Xu3;  LI Fang-qin1
(1.the Affiliated Hospital of Yan’an University, Shaanxi Yan’an 716000, China; 2. Medical College of Yan’an University, Shaanxi Yan’an 716000, China; 3. the Second People’s Hospital of Yan’an City, Shaanxi Yan’an 716000,China)
关键词:
新型冠状病毒肺炎（COVID-19）; 多样本; 严重急性呼吸综合征冠状病毒2（SARS-CoV-2）; 逆 转录酶- 聚合酶链锁反应（RT-PCR）
分类号:
R373.1；R392.11
DOI:
10.3969/j.issn.1671-7414.2020.03.022
摘要:
目的　探讨不同类型样本新冠核酸检测结果在新型冠状病毒肺炎（COVID-19）临床诊治和疫情防控中的意义。 方法　选取 2020 年1 月23 日~2 月22 日延安大学附属医院确诊的5 例COVID-19 患者，采集不同部位标本进行核酸 检测，并进行统计学分析。结果　采集的123 例标本严重急性呼吸综合征冠状病毒2（SARS-CoV-2）核酸检测结果表明, 咽拭子25 例，阳性率44.0％（11/25）; 鼻拭子40 例，阳性率25.0％（10/40); 肛拭子40 例, 阳性率70.0％（28/40)；18 例血标本核酸检测均阴性，5 例患者符合出院标准时粪便核酸检测均阳性。截止3 月5 日对5 例患者出院后追踪发现， 出院2 周或更长时间粪便 SARS-CoV-2 核酸仍阳性。结论　多样本新冠核酸检测对于新冠感染患者的确诊有重要价值； 患者治愈出院后应加强随访、教育和复查，做好排泄物的管理，有利于巩固疫情防控效果，减少接触感染的发生。
Abstract:
Objective　To investigate the significance of the detection results of different types of samples in the clinical diagnosis and treatment of new coronavirus pneumonia (COVID-19) and the prevention and control of epidemic situation. Methods 　From January 23, 2020 to February 22, 2020, 5 patients with COVID-19 were selected from the Affiliated Hospital of Yan’an University to collect samples from different parts for nucleic acid detection and statistical analysis. Results　The results showed that 25 throat swabs were positive in 44.0% (11 / 25), 40 nose swabs were positive in 25.0% (10 / 40), 40 anal swabs were positive in 70.0% (28 / 40). 18 blood samples were negative in nucleic acid test, and 5 patients were positive in fecal nucleic acid test when they met the discharge standard. As of March 5, 5 patients were found to be positive for COV-2 nucleic acid in stool for 2 weeks or longer after discharge. Conclusion　The detection of different kinds of new crown nucleic acid has important value for the diagnosis of new crown infection. The patients should strengthen follow-up, education and reexamination after cure and discharge, and manage excreta well, which is helpful to consolidate the effect of epidemic prevention and control and reduce the occurrence of contact infection.

参考文献/References:

[1]　WHO Director-General’s remarks at the media briefing on 2019-nCoV on 11 February 2020[C].2020-02-11. http://www.who.int/dg/speeches/detail/who-directorgeneral- s-remarks-at-the-media-briefing-on-2019- ncov-on-11-february-2020? fbclid=IwAR3ZwwW-_ arNeH2eifar ZKvOIxw2LH9Dc7KXdwFI_ Fx4qpDKKE_u9qXpSHo.
[2]　王成彬. 核酸检测用于确诊新型冠状病毒肺炎阳性率 低的原因分析[J/OL].中华医学杂志,2020,100(13):961- 964. WANG Chengbin. Analysis of low positive rate of nucleic acid detection method used for diagnosis for novel coronavirus pneumonia [J]. National Medical Journal of China, 2020,100(13) :961-964.
[3]　莫茜, 秦炜, 傅启华, 等. 正确认识新型冠状病 毒核酸检测的影响因素[J]. 中华检验医学杂 志,2020,43(3):213-216. MO Xi, QIN Wei, FU Qihua, et al. A correct understanding of the influencing factors of new coronavirus nucleic acid detection [J/OL]. Chinese Journal of Laboratory Medicine, 2020,43(3):213-216.
[4]　童永清，汪明，徐万洲，等. 新型冠状病毒核酸检 测临床实验室操作规范的建议[J/OL]. 中华检验医 学杂志，2020,43(3):209-212. TONG Yongqing, WANG Ming, XU Wanzhou, et al. Proposal for detection of 2019-nCoV nucleic acid in clinical Laboratories [J/OL]. Chin J Lab Med, 2020,43(3):209-212.
[5]　中华医学会检验医学分会.2019 新型冠状病 毒病毒核酸检测专家共识[J/OL]. 中华医学杂 志,2020,100(13):968-973. Chinese Medical Association Laboratory Medicine Branch. Expert consensus on nucleic acid detection of new coronavirus pneumonia virus [J/OL]. National Medical Journal of China, 2020,100(13):968-973.
[6]　国家卫生健康委员会办公厅. 新型冠状病毒感染的 肺炎诊疗方案（试行第七 版） [EB/OL].(2020-03- 03)http://www.nhc-gov.cn/yzygi/s7653p/202003/46c 9294a7dfe4cef80dc7f5912eb1989.shtml. Office of the National Health Council. Diagnosis and Treatment of Pneumonia with New Coronavirus Infection (Trial Version 7) [EB/OL].(2020-03-03) http://www.nhc-gov.cn/yzygi/s7653p/202003/46c 9294a7dfe4cef80dc7f5912eb1989.shtml. [7 ] 张瑞, 李金明. 如何减少新型冠状病毒核酸检测的 看看“假阴性”[J]. 中华医学杂志,2020,100(11):801- 804. ZHANG Rui, LI Jinming.The way to reduce the“folse negative results” of 2019 novel coronavirus nucleic acid detection [J/OL]. Natl Med J China, 2020,100(11):801-804
[8]　 任彬彬, 陈海君, 张德和, 等. 新型冠状病毒肺炎恢 复期咽拭子核酸检测阴性但粪便检测阳性三例分析 [J]. 中华临床感染病杂志,2020,13(1):33-35, 38. REN Binbin, CHEN Haijun, ZHANG Dehe, et al. Three cases of CDVID-19 with viral nucleic acid negative in pharynx swab and positive in stool specimens during recovery period [J]. Chinese Journal of Clinical Infectius Diseases ,2020,13(1): 33-35,38.
[9]　 杨智炜, 李干文, 戴小灵, 等. 新型冠状病毒肺炎咽 拭子核酸检测转阴但粪便核酸检测仍阳性三例[J]. 中华消化杂志,2020,40(2):77-79. YANG Zhiwei, LI Ganwen, DAI Xiaoling, et al. Three cases of novel coronavirus pneumonia with viral nucleic acids still positive in stool after throat swab detection turned negative[J]. Chinese Journal of Digestion ,2020,40(2): 77-79.

备注/Memo

备注/Memo:
收稿日期：2020-04-21 修回日期：2020-05-11基金项目：延安市重点科研计划（SL2020ZCSY-002）; 延安大学科研计划（YDKYYJ04）。 作者简介：杜伟平（1985-），男，硕士，主管检验师，研究方向：基因多态性与疾病，E-mail:724411083@qq.com。 通讯作者：李芳芹（1963-），女，本科，主任检验师，研究方向：病原微生物快速诊断，E-mail:1694092353@qq.com。
更新日期/Last Update: 2020-07-20