[1]郭晓波a,蔺京a,史敏,等.T 细胞型急性淋巴细胞白血病患者HOX11L2 基因的表达及其临床意义[J].现代检验医学杂志,2020,35(05):9-12.[doi:10.3969/j.issn.1671-7414.2020.05.003]
　GUO Xiao-boa,LIN Jinga,SHI Min,et al.Expression and Clinical Significance of HOX11L2 Gene in Patients with T Cell Acute Lymphoblastic Leukemia[J].Journal of Modern Laboratory Medicine,2020,35(05):9-12.[doi:10.3969/j.issn.1671-7414.2020.05.003]
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T 细胞型急性淋巴细胞白血病患者HOX11L2 基因的表达及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年05期
页码:
9-12
栏目:
论著
出版日期:
2020-11-01

文章信息/Info

Title:
Expression and Clinical Significance of HOX11L2 Gene in Patients with T Cell Acute Lymphoblastic Leukemia
文章编号:
1671-7414（2020）05-009-04
作者:
郭晓波1a; 蔺京1a; 史敏2; 杨乐1b; 张养民1c; 杨瑞利3
1. 西安交通大学附属西安市中心医院a. 血液科；b. 肿瘤科；c. 输血科， 西安 710004； 2. 西安培华学院医学院，西安 710065；3. 西安长安医院检验科，西安 710016
Author(s):
GUO Xiao-bo1a; LIN Jing1a; SHI Min2; YANG Le1b; ZHANG Yang-min1c; YANG Rui-li3
1a. Department of Hematology;1b. Department of Oncology; 1c. Department of Blood transfusion，Xi’an Central Hospital Affiliated to Xi’an Jiaotong University,Xi’an 710004, China；2. Medical College of Xi’an Peihua University， Xi’an 710065,China;3. Department of Clinical Laboratory, Xi’an Chang’an Hospital，Xi’an 710016,China
关键词:
急性淋巴细胞白血病; 染色体; HOX11L2 基因; 实时荧光定量PCR
分类号:
R557.4；Q786
DOI:
10.3969/j.issn.1671-7414.2020.05.003
文献标志码:
A
摘要:
目的　研究T 细胞型急性淋巴细胞白血病（acute lymphoblastic leukemia, ALL）患者HOX11L2 基因的表达及 其临床意义。方法　采用回顾性分析方法对50 例患者性别、年龄、白细胞数量、FAB 分类（L1,L2 and L3）、分子生 物学特点以及生存时间进行分析，观察HOX11L2 基因表达与临床特点及预后关系。结果　50 例T 细胞型急性淋巴细胞 白血病患者中，表达HOX11L2 基因患者为12 例（24%），其中男性7 例（58.3%），女性5 例（41.7%），HOX11L2 基因表达者在男女性别之间的比较，差异无统计学意义（χ2=0.119，P=0.730）。表达HOX11L2 基因患者年龄在5~10 岁间居多，表达率为58.3%，与其它年龄组之间的比较，差异有统计学意义（χ2=11.577，P=0.018）。HOX11L2 基因表 达患者中白细胞计数≤ 50×109/L 的患者10 例（83.3%），白细胞计数>50×109/L 的患者2 例（16.7%），两组比较差 异有统计学意义（χ2=5.469，P=0.019）。FAB 分类中，有75% 的HOX11L2 基因表达患者为L1 型，与L2 和L3 组相 比较，差异有统计学意义（χ2=8.766，P=0.008）。其中34 例获得随访资料的患者，HOX11L2 基因表达组比非表达组 生存时间短（中位生存时间分别为8.0 个月和11.0 个月），两组差异具有统计学意义（P<0.05）。结论　研究结果表明 HOX11L2 基因主要表达于T-ALL 患者，且以L1 型儿童T-ALL 为主，HOX11L2 基因表达者预后不良，生存时间短。 临床上通过HOX11L2 基因检测结果与常规细胞形态学、遗传学、荧光原位杂交等技术检测结果相结合，将对急性淋巴 细胞白血病的鉴别诊断、分型、预后及微小残留病检测等具有十分重要的意义。
Abstract:
Objective　To study the expression and clinical significance of HOX11L2 gene in patients with T-cell acute lymphoblastic leukemia（T-ALL）. Methods　The sex，age，eukocyte count，FAB classification (L1，L2 and L3)， molecular biological characteristics and survival time of 50 patients were analyzed retrospectively. The relationship between HOX11L2 gene expression，clinical characteristics and prognosis was observed. Results　Among the 50 patients with T-ALL， 12 (24%) expressed HOX11L2 gene，7 (58.3%) males and 5 (41.7%) females. There was no significant difference between the two groups in gender (χ2=0.119， P=0.730). Most of the patients with HOX11L2 gene were aged 5~10 years old，the expression rate was 58.3%，compared with other patients, the difference between the age groups was statistically significant (χ2=11.577， P=0.018).Among the patients with HOX11L2 gene expression，10 patients (83.3%) had WBC count ≤ 50 × 109 / L，and 2 patients (16.7%) had WBC count > 50 × 109 / L，and the difference between the two groups was statistically significant (χ2=5.469，P=0.019). In the FAB classification，75% of the patients with HOX11L2 gene expression were L1 type，and the difference was statistically significant compared with L2 and L3 groups (χ2=8.766，P=0.008). Among the 34 patients who obtained follow-up data，the survival practice of HOX11L2 gene expression group was shorter than that of non expression group (median survival time was 8.0 months and 11.0 months respectively)，and the difference between the two groups was statistically significant (P < 0.05). Conclusion　The results showed that HOX11L2 gene was mainly expressed in T-ALL patients，and it was mainly expressed in L1 children. The prognosis of HOX11L2 gene expression patients was poor，and the survival time was short. Clinically，the results of HOX11L2 gene detection combined with the results of conventional cytomorphology， genetics，fluorescence in situ hybridization and other technologies will be used for the differential diagnosis，typing，prognosis and microanalysis of acute lymphoblastic leukemia Detection of small residual diseases is of great significance.

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备注/Memo

备注/Memo:
基金项目：陕西省科技厅重点研发计划项目(2018SF-205)。
作者简介：郭晓波（1981-），男，硕士研究生，副主任检验技师，主要从事白血病分子诊断研究，E-mail：258442579@qq.com。
通讯作者：杨瑞利，女，本科，副主任检验技师，主要从事临床检验工作，E-mail：172454089@qq.com。
更新日期/Last Update: 2020-10-30