[1]唐雄驰,徐庆萍,韦小荣,等.广西壮族自治区人群华支睾吸虫感染与MN 血型基因的相关性研究[J].现代检验医学杂志,2020,35(05):16-19，136.[doi:10.3969/j.issn.1671-7414.2020.05.005]
　TANG Xiong-chi,XU Qing-ping,WEI Xiao-rong,et al.Correlation between Clonorchis Sinensis Infection and MN Blood Type Gene in Guangxi Zhuang Autonomous Region[J].Journal of Modern Laboratory Medicine,2020,35(05):16-19，136.[doi:10.3969/j.issn.1671-7414.2020.05.005]
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广西壮族自治区人群华支睾吸虫感染与MN 血型基因的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年05期
页码:
16-19，136
栏目:
论著
出版日期:
2020-11-01

文章信息/Info

Title:
Correlation between Clonorchis Sinensis Infection and MN Blood Type Gene in Guangxi Zhuang Autonomous Region
文章编号:
1671-7414（2020）05-016-05
作者:
唐雄驰1; 徐庆萍1; 韦小荣2; 黄克河1; 江毅1; 施超园1; 梁延连3
1. 贵港市平南区人民医院，广西贵港 537300；2. 广西医科大学附属武鸣医院；南宁 537000； 3. 深圳市血液中心输血医学研究所, 广东深圳 518000）
Author(s):
TANG Xiong-chi1; XU Qing-ping1;  WEI Xiao-rong2; HUANG Ke-he1;  JIANG Yi1;  SHI Chao-yuan1;  LIANG Yan-lian3
1.People’s Hospital of Pingnan District of Guigang, Guangxi Guigang 537300, China；2.Wuming Hospital Affiliated to Guangxi Medical University, Nanning 537000,China;3.Institute of Transfusion Medicine,Shenzhen Blood Center, Guangdong Shenzhen 518000, China
关键词:
华支睾吸虫; 血型糖蛋白; M; N; MG; MT 基因频率
分类号:
R383.22；R457.11
DOI:
10.3969/j.issn.1671-7414.2020.05.005
文献标志码:
A
摘要:
目的　调查广西壮族自治区人群华支睾吸虫高发人群与低感染人群的分布状态及MN 血型基因的相关性，深 入了解华支睾吸虫的感染情况，为预防与治疗华支睾吸虫病打下基础。方法　以广西壮族自治区的武鸣区、贵港平南区 两个代表性地区作为调查研究对象，以酶联免疫法随机筛查贵港平南区500 例、武鸣区700 例的15~65 岁居民，分别 采集3ml 抗凝血与3ml 不抗凝血，共检测1 200 例人群血清中的华支睾吸虫IgG 抗体；通过血型血清学检测样本的红细 胞血型糖蛋白MN 血型，用基因分型的方法鉴定MG 与MT 的型别。结合两个区的调查总人数、华支睾吸虫抗体阳性率、 阴性率、MN 血型的血清学与基因分型作为统计数据。结果　两个具有相同生活饮食习惯地区人群的华支睾吸虫感染率 具有显著差异，即武鸣区华支睾吸虫感染率高达62%，其M 基因频率、N 基因频率、MG 基因频率和MT 基因频率分别 为72%，28%，68.6% 和31.4%。贵港平南区华支睾吸虫阳性感染率只有3.4%，其M 基因频率、N 基因频率、MG 基因 频率和MT 基因频率分别为69%，31%，82.3% 和17.7%。结论　广西壮族自治区华支睾吸虫的感染率具有地区间的明 显差异，而红细胞糖蛋白血型的M，N，MG 和MT 基因频率未发现明显差异，另外未发现饮食习惯与两区华支睾吸虫 的易感因素直接相关。
Abstract:
Objective　To investigate the distribution of Clonorchis sinensis in high incidence and low infection population in Guangxi Zhuang Autonomous Region and the correlation of MN blood group gene, so as to understand the infection situation of Clonorchis sinensis, and lay a foundation for the prevention and treatment of Clonorchis sinensis.Methods　Two representative regions that Wuming and Guigang(Pingnan) District for the Guangxi Zhuang Autonomous Region were taken as the research objects.There were 1 200 residents aged 15~65 years old that 500 cases in Pingnan District of Guigang and 700 cases in Wuming. The anticoagulant and nonanticoagulant blood for 3ml were collected and IgG antibodies of Clonorchis sinensis were detected by enzyme linked immunoassay (ELISA). The MN blood type was detected and the MG and MT types were identified by genotyping.Combined with the total number of people investigated in the two areas with the positive rate,negative rate of antibodies against Clonorchis sinensis and the serological and genotyping of MN blood type were taken as the statistical data. Results　 The infection rate of Clonorchis sinensis was significantly differentin two regions with the same living and eating habits. The infection rate of Clonorchis sinensis in Wuming area was as high as 62%,and the M gene frequency,the N gene frequency,the MG gene frequency frequency and the MT gene frequency frequency were 72%，28%，68.6% and 31.4%,respectively. But there’s a difference in Pingnan District of Guigang were the infection rate of Clonorchis sinensis was 3.4%, and the M gene frequency, the N gene frequency gene,the MG gene frequency and the MT gene frequency were 69%，31%，82.3% and 17.7%, respectively. Conclusion　The infection rate of Clonorchis sinensis in two populations with the same living and eating habits was significantly different. But no significant difference was found about erythrocyte glycoprotein blood group M, N, MG and MT gene frequency. In addition, there was no direct correlation between dietary habits and susceptibility factors of Clonorchis sinensis.

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备注/Memo

备注/Memo:
基金项目：贵港平南市科技局资助项目（贵科攻 1803027）；贵港平南市科技局立项项目（贵科转1803016）。
作者简介：唐雄驰（1974-），男，副主任医师，主要从事消化内科疾病的诊治研究，E-mail：pnrmtxc@163.com。
通讯作者：梁延连（1973-），女，副主任技师，深圳市血液中心输血医学研究所，主要从事输血医学与红细胞血型免疫遗传研究， E-mail：lianviky@126.com。
更新日期/Last Update: 2020-10-30