[1]何晓一a,李春燕b.2 型糖尿病患者锰超氧化物歧化酶Ala-9Val 基因多态性与颈动脉粥样硬化的相关性研究[J].现代检验医学杂志,2020,35(05):24-27.[doi:10.3969/j.issn.1671-7414.2020.05.007]
　HE Xiao-yia,LI Chun-yanb.Association between Manganese Superoxide Dismutase Gene Ala-9Val Polymorphism and Carotid Atherosclerosis in Patients with Type 2 Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2020,35(05):24-27.[doi:10.3969/j.issn.1671-7414.2020.05.007]
点击复制

2 型糖尿病患者锰超氧化物歧化酶Ala-9Val 基因多态性与颈动脉粥样硬化的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年05期
页码:
24-27
栏目:
论著
出版日期:
2020-11-01

文章信息/Info

Title:
Association between Manganese Superoxide Dismutase Gene Ala-9Val Polymorphism and Carotid Atherosclerosis in Patients with Type 2 Diabetes Mellitus
文章编号:
1671-7414（2020）05-024-04
作者:
何晓一a; 李春燕b
中国人民解放军联勤保障部队第九八七医院a. 内分泌科；b. 消化内科, 陕西宝鸡 721000
Author(s):
HE Xiao-yia;  LI Chun-yanb
a. Department of Endocrinologists; b.Department of Gastroenterology, No.987 Hospital of the Joint Service Support force of the Chinese People’s Liberation Army, Shaanxi Baoji 721000, China
关键词:
2 型糖尿病 (T2DM); 锰超氧化物歧化酶 (Mn-SOD); 颈动脉粥样硬化 (CAS); Ala-9Val 基因多态性
分类号:
R587.1；R543.5；Q786
DOI:
10.3969/j.issn.1671-7414.2020.05.007
文献标志码:
A
摘要:
目的　探讨2 型糖尿病 (type 2 diabetes mellitus, T2DM) 患者锰超氧化物歧化酶(manganese-superoxide dismutase, Mn-SOD)Ala-9Val 基因多态性与颈动脉粥样硬化(carotid atherosclerosis, CAS) 的关系。方法　选取九八七医院2017 年1 月~2019 年1 月的310 例T2DM 患者为T2DM 组，根据颈动脉超声检查结果分为无CAS 亚组和CAS 亚组。另随机选 取同期160 例健康体检的志愿者作为对照组，采用聚合酶链反应- 限制性片段长度多态性法(polymerase chain reaction restriction fragment length polymorphism, PCR-RFLP) 检测Mn-SOD 基因Ala-9Val 位点基因型。分别比较各组间Ala- 9Val 位点基因型和等位基因分布频率。采用Logistic 多因素回归分析法探究T2DM 患者发生CAS 的独立危险因素。结 果　对照组和T2DM 组的Ala-9Val 位点基因型分布频率差异无统计学意义(χ2=4.210，P=0.122)，两组的等位基因分布 频率差异具有统计学意义(χ2=4.487，P=0.034)。CAS 亚组与无CAS 亚组的Ala-9Val 位点基因型和等位基因分布频率差 异均具有统计学意义(χ2=12.283，P=0.002；χ2=10.984，P=0.001)。Logistics 多因素回归分析结果显示，Val/Val 基因型 是T2DM 患者发生CAS 的独立危险因素(OR=1.865，95%CI 1.086~3.200，P=0.007)。结论　T2DM 患者Mn-SOD 基因 Ala-9Val 位点多态性与CAS 存在一定程度的相关性。
Abstract:
Objective　To investigate the relationship between the Ala-9Val gene polymorphism of manganese superoxide dismutase (Manganese-Superoxide Dismutase,Mn-SOD) and carotid atherosclerosis (CAS) in patients with type 2 diabetes mellitus (T2DM). Methods　A total of 310 cases of T2DM patients in No.987 Hospital from January 2017 to January 2019 were assigned to the T2DM group. According to the results of carotid ultrasound, the subjects were divided into no CAS subgroup and CAS subgroup. Besides, 160 healthy volunteers during the same period were randomly assigned to the control group.PCR RFLP was used to detect the Ala-9 Val locus genotype of MN-SOD gene. Allele frequency and genotype of Ala-9Val locus were compared among groups. Logistic multivariate regression analysis was used to explore the independent risk factors for CAS in T2DM patients. Results　There was no significant difference in Ala-9Val genotype distribution frequency between the control group and the T2DM group (χ2=4.210,P=0.122), and the allele frequency difference between the two groups was statistically significant (χ2=4.487,P=0.034). There were statistically significant differences in Ala-9Val genotype and allele distribution frequency between the CAS subgroup and the non-CAS subgroup (χ2=12.283, P=0.002; χ2=10.984, P=0.001). Logistic multifactor regression analysis results showed that Val/Val genotype was an independent risk factor for CAS in T2DM patients (OR=1.865,95%CI 1.086~3.200,P=0.007). Conclusion　There was a certain degree of correlation between the polymorphism of Mn-SOD gene ALa-9Val and CAS in T2DM patients.

参考文献/References:

[1] NEWSHOLME P, CRUZAT V F, KEANE K N, et al. Molecular mechanisms of ROS production and oxidative stress in diabetes[J]. The Biochemical Journal, 2016, 473(24): 4527-4550.
[2] KAHYA M C, NAZIRO?LU M, ?VEY ? S. Modulation of diabetes-induced oxidative stress, apoptosis, and Ca2+ entry through TRPM2 and TRPV1 channels in dorsal root ganglion and hippocampus of diabetic rats by melatonin and selenium [J]. Molecular neurobiology, 2017,54(3): 2345-2360.
[3] D’SOUZA J M P, D’SOUZA R P, VIJIN V F, et al. High predictive ability of glycated hemoglobin on comparison with oxidative stress markers in assessment of chronic vascular complications in type 2 diabetes mellitus[ J]. Scandinavian Journal of Clinical and Laboratory Investigation, 2016, 76(1): 51-57.
[4] 周玉, 王伶俐, 黄杨, 等．糖尿病大鼠的SOD 活性 与抗氧化剂治疗对机体抗氧化状态的影响[J]. 中国 实验动物学报, 2016, 24(4): 422-426. ZHOU Yu, WANG Lingli, HUANG Yang, et al. Effect of antioxidant treatment on the expression and activity of SOD in diabetic rats[J]. Acta Laboratorium Animalis Scientia Sinica, 2016, 24(4): 422-426.
[5] 李俊燕, 陶枫, 吴新星, 等．锰超氧化物歧化酶基因 多态性与2 型糖尿病的相关性[J]. 中国老年学杂志, 2016, 36(4): 832-833. LI Junyan, TAO Feng, WU Xinxing, et al. The association between Manganese superoxide dismutase gene polymorphism and type 2 diabetes mellitus [J]. Chinese Journal of Gerontology, 2016, 36(4): 832-833.
[6] TIWARI A K, PRASAD P, THELMA B K, et al. Oxidative stress pathway genes and chronic renal insufficiency in Asian Indians with type 2 diabetes[J]. Journal of Diabetes and Its Complications, 2009, 23(2): 102-111.
[7] 中华医学会糖尿病学分会．中国2 型糖尿病防治指 南（2013 年版）[J]. 中华糖尿病杂志, 2014, 22(8): 447-498. Chinese Diabetes Society Chinese guidelines for the prevention and treatment of type 2 diabetes(2013 edition)[J]. Chinese Journal of Diabetes, 2014, 22(8): 447-498.
[8] HAN C Y. Roles of reactive oxygen species on insulin resistance in adipose tissue[J]. Diabetes Metab J， 2016, 40 (4) :272-279.
[9] ANDERSON C, MAJESTE A, HANUS J, et al. E-Cigarette aerosol exposure induces reactive Oxygen species, DNA damage, and cell death in vascular endothelial cells[J]. Toxicological Sciences, 2016, 154(2): 332- 340.
[10] TOGLIATTO G， DENTELLI P， ROSSO A, et al. PDGF-BB carried by endothelial cell–derived extracellular vesicles reduces vascular smooth muscle cell apoptosis in diabetes[J]. Diabetes, 2018, 67(4): 704- 716.
[11] 张瑞. 血清超氧化物歧化酶检测在糖尿病肾病中的 应用价值[J]. 延安大学学报（医学科学版）, 2014, 12(4): 47-49. ZHANG Rui. Application value of detection of serum superoxide dismutase in diabetes mellitus[J]. Journal of Yanan University(Medical Sciences), 2014, 12(4): 47- 49.
[12] 季雄娟，邵静，陆胜．糖化血红蛋白及糖化清蛋白 水平对糖尿病视网膜病变的诊断价值分析[J]．现代 检验医学杂志, 2018, 33(5): 77-81. JI Xiongjuan, SHAO Jing, LU Sheng. Diagnostic value of glycocsylated hemoglobin A1c and glycated albumin levels in diabetic retinopany[J]. Journal of Modern Laboratory Medicine, 2018, 33(5): 77-81.
[13] 姚创利，赵佳，鲁旭娟，等．冠心病患者血清脂蛋 白相关磷脂酶A2 水平与锰超氧化物歧化酶9 Ala ／ Val 基因多态性关系探讨[J]. 现代检验医学杂志, 2017, 32(1): 26-29. YAO Chuangli, ZHAO Jia, LU Xujuan, et al. Study on the relationship between lipoprotein associated phospholipase A2 level and manganese superoxide dismutase 9 Ala/Val genetic polymorphism in patients with coronary heart disease [J]. Journal of Modern Laboratory Medicine, 2017, 32(1): 26-29.
[14] KALKAN G, SE?KIN HY , BENLI ?，et al. Relationship between Manganese superoxide dismutase(MnSODAla- 9Val)and glutathione peroxidase(GPx1 Pro 197 Leu)gene polymorphisms and alopecia areata[J]. Int J Clini Exp Med, 2015, 8(11): 21533-21540.
[15] JEROTIC D, MATIC M, SUVAKOV S, et al. Association of Nrf2, SOD2 and GPX1 polymorphisms with biomarkers of oxidative distress and survival in End- Stage renal disease patients[J]. Toxins, 2019, 11(7): 431.
[16] DRAGICEVIC B, SUVAKOV S, JEROTIC D, et al. Association of SOD2 (rs4880) and GPX1 (rs1050450) gene polymorphisms with risk of balkan endemic nephropathy and its related tumors[J]. Medicina, 2019, 55(8): E435.

备注/Memo

备注/Memo:
作者简介：何晓一(1980- )，女，本科，主治医师，研究方向：内分泌糖尿病代谢，E-mail:baojihexiaoyi@163.com。
通讯作者：李春燕(1980- )，女，本科，主治医师，研究方向：消化道内镜，E-mail:242980283@qq.com。
更新日期/Last Update: 2020-10-30