[1]樊春荔,何燕娟.缺血性脑卒中和短暂性脑缺血发作患者血清miR-23b-3p 水平变化的比较研究[J].现代检验医学杂志,2020,35(05):51-54,123.[doi:10.3969/j.issn.1671-7414.2020.05.013]
　FAN Chun-li,HE Yan-juan.Altered Levels of Serum miR-23b-3p in Patients with Ischemic Stroke and Transient Ischemic Attack[J].Journal of Modern Laboratory Medicine,2020,35(05):51-54,123.[doi:10.3969/j.issn.1671-7414.2020.05.013]
点击复制

缺血性脑卒中和短暂性脑缺血发作患者血清miR-23b-3p 水平变化的比较研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年05期
页码:
51-54,123
栏目:
论著
出版日期:
2020-11-01

文章信息/Info

Title:
Altered Levels of Serum miR-23b-3p in Patients with Ischemic Stroke and Transient Ischemic Attack
文章编号:
1671-7414（2020）05-051-05
作者:
樊春荔; 何燕娟
常州市第一人民医院，江苏常州 210002
Author(s):
FAN Chun-li;  HE Yan-juan
the First People’s Hospital of Changzhou, Jiangsu Changzhou 210002, China
关键词:
短暂性脑缺血发作; 缺血性卒中; 微小核糖核酸; 生物标志物
分类号:
R743.3；R392.11
DOI:
10.3969/j.issn.1671-7414.2020.05.013
文献标志码:
A
摘要:
目的　探讨短暂性脑缺血发作(transient ischemic attack, TIA) 患者和缺血性脑卒中(ischemic stroke, IS) 患者血 清miR-23b-3p 的水平变化。方法　选取常州市第一人民医院神经内科2017 年8 月~ 2019 年4 月收治的156 例IS 患 者，71 例TIA 患者和85 例常规体检的健康对照者纳入研究。采用实时荧光定量PCR(quantitative reverse-transcription PCR, qRT-PCR) 技术检测受试者血清miR-23b-3p 水平并分析相关生化指标。结果　与健康对照组相比，IS 组(t=4.517, P<0.001) 及TIA 患者组 (t=2.728, P<0.001) 血清miR-23b-3p 水平均显著升高， 且IS 组高于TIA 患者组 (t=3.364, P=0.043)。此外，重度IS 组miR-23b-3p 水平明显高于中度IS(t=1.212, P=0.034) 和轻度IS 组(t=4.931, P<0.001)，差 异均有统计学意义。相关性分析显示IS 患者血清miR-23b-3p 水平与美国国立卫生研究院卒中量表(National Institutes ofHealth Stroke Scale, NIHSS) 评分呈正相关(r=0.453, P<0.001)。ROC 曲线分析显示，血清miR-23b-3p 区分IS 患者 与健康对照者的AUC 为0.817 (95% CI: 0.762 ～ 0.873, P<0.001)；区分TIA 患者与健康对照者的AUC 为0.789 (95% CI: 0.716 ～ 0.861, P<0.001)。Logistic 回归分析显示，在校正了年龄、性别、总胆固醇(total cholesterol, TC) 及三酰甘 油(triglyceride, TG) 水平的影响后，血清miR-23b-3p 水平升高与IS(OR=6.045, 95% CI : 2.011 ～ 12.080, P=0.008) 及 TIA(OR=2.012, 95% CI : 1.984 ～ 7.042, P=0.002) 的发生相关。结论　IS 患者和TIA 患者血清miR-23b-3p 水平均显著增 高且IS 患者水平变化较TIA 患者更显著，miR-23b-3p 可能成为病情评估与判断的潜在标志物。
Abstract:
Objective　To investigate altered levels of serum miR-23b-3p in ischemicstroke (IS) and transient ischemic attack (TIA). Methods　A total of 71 TIA patients, 156 IS patients who were admitted to the First People’s Hospital of Changzhou from August 2017 to April 2019 and 85 healthy were enrolled. Serum miR-23b-3p levels were determined by quantitative reversetranscription PCR (qRT-PCR) and serumbiochemistry biomarkers were also analysed. Results　Compared with the controls, serum miR-23b-3p levels in TIA patients (t=2.728, P<0.001) and IS patients (t=4.517, P<0.001) were significantly increased. The miR-23b-3p levels in IS patients were higher than in TIA patients (t=3.364, P=0.043). Additionally, the level of miR-23b-3p was found to be significantly increased in severe IS compared with moderate IS (t=1.212, P=0.034) and mild IS (t=4.931, P<0.001) and the difference was statistically significant. Serum miR-23b-3p were positively correlated with the National Institutesof Health Stroke Scale (NIHSS) scores (r=0.453, P<0.001). ROC curve analysis showed that the AUC of miR-23b-3p for differentiating IS patients and controls was 0.817 (95% CI: 0.762 ～ 0.873, P<0.001) and the AUC for differentiating TIA patientsand controls was 0.789 (95% CI: 0.716 ～ 0.861, P<0.001). Logistic regression analysis revealed that high miR-23b-3p levels were closely associated with the presence of IS (OR=6.045, 95% CI :2.011 ～ 12.080, P=0.008) and TIA (OR=2.012, 95% CI : 1.984 ～ 7.042, P=0.002) after adjustment for the age, gender, total cholesterol (TC) and triglyceride (TG) levels. Conclusion　Serum miR-23b-3p levels were significantly elevated in IS patients and TIA patients. Serum miR-23b-3p levels may be function as the potential biomarker for the disease assessment and udgement．

参考文献/References:

[1]　 KINGSBURY C, HEYCK M, BONSACK B, et al. Stroke gets in your eyes: stroke-induced retinal ischemia and the potential of stem cell therapy[J]. Neural Regeneration Research, 2020, 15(6): 1014-1018.
[2]　 World Health Organization . World Health Statistics 2016: Monitoring Health for the SDGs, Sustainable Development Goals[M]. Geneva: Switzerland, 2016, 41:293-328.
[3]　 EASTON J D, SAVER J L, ALBERS G W, et al. Definition and evaluation of transient ischemic attack: a scientific statement for healthcare professionals from the American Heart Association/American Stroke Association Stroke Council; Council on Cardiovascular Surgery and Anesthesia; Council on Cardiovascular Radiology and Intervention; Council on Cardiovascular Nursing; and the Interdisciplinary Council on Peripheral Vascular Disease. The American Academy of Neurology affirms the value of this statement as an educational tool for neurologists[J]. Stroke, 2009, 40(6): 2276-2293.
[4]　 GASPARELLO J, LAMBERTI N, PAPI C, et al. Altered erythroid-related miRNA levels as a possible novel biomarker for detection of autologous blood transfusion misuse in sport[J]. Transfusion,2019,59(8): 2709-2721.
[5]　 GHOREISHY A, KHOSRAVI A, GHAEMMAGHAMI A. Exosomal microRNA and stroke: A review[J]. Journal of Cellular Biochemistry, 2019, 120(10): 16352-16361.
[6]　 AHIR B K, OZER H, ENGELHARD H H, et al. MicroRNAs in glioblastoma pathogenesis and therapy: A comprehensive review[J]. Critical Reviews in Oncology / Hematology,2017,120: 22-33.
[7]　 MA Feifei,ZHANG Xuejing,YIN Kejie. MicroRNAs in central nervous system diseases: A prospective role in regulating blood-brain barrier integrity[J]. Experimental Neurology,2020,323: 113094.
[8]　 LI Jia, LI Keshen, CHEN Xiangyan. Inflammationregulatory microRNAs: Valuable targets for intracranial atherosclerosis[J]. Journal of Neuroscience Research, 2019, 97(10): 1242-1252.
[9]　 MARTINEZ B, PEPLOW P V. MicroRNAs in blood and cerebrospinal fluid as diagnostic biomarkers of multiple sclerosis and to monitor disease progression[J]. Neural Regeneration Research, 2020, 15(4): 606-619.
[10] DONG Ping, ZHANG Xiyan, ZHAO Jian, et al. AntimicroRNA- 132 causes sevoflurane-induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway [J]. International Journal of Molecular Medicine, 2018, 42(6): 3238-3246.
[11] SUN Yiwen, ZHU Zexuan, YOU Zhuhong, et al. FMSM: a novel computational model for predicting potential miRNA biomarkers for various human diseases[J]. BMC Systems Biology, 2018, 12(Suppl 9): 121.
[12] 姜彦峰，李小侠，陈安志．颈动脉粥样硬化斑块性质 与患者血清miRNA-126,miRNA-155 水平的相关性研究 [J]．现代检验医学杂志，2017，32（6）：74-77, 80. 　　 JIANG Yanfeng, LI Xiaoxia, CHEN Anzhi. Clinical application of serum miRNA-126,miRNA-155 detection in evaluation of plaque property in the carotid atherosclerotic desease[J]. Journal of Modern Laboratory Medicine, 2017,32(6):74-77,80.
[13] 常运鹤，王庆贤．循环微小RNA 在静脉血栓栓塞 症中的研究进展[J]. 中华实验外科杂志，2019, 36（2）: 389-393． 　　 CHANG Yunhe, WANG Qingxian. Research progress of circulating microRNA in venous thromboembolism [J]. Chinese Journal of Experimental Surgery, 2019, 36(2):389-393.
[14] QU Yi, WU Jinlin, CHEN Dapeng, et al. MiR-139- 5p inhibits HGTD-P and regulates neuronal apoptosis induced by hypoxia-ischemia in neonatal rats [J]. Neurobiology of Disease, 2014, 63: 184-193.
[15] IRMADY K, JACKMAN K A, PADOW V A, et al. Mir-592 regulates the induction and cell death-promoting activity of p75NTR in neuronal ischemic injury[J]. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 2014, 34(9): 3419-3428.
[16] CHEN Qun, ZHANG Fan, WANG Yanbo, et al. The transcription factor C-Myc suppresses miR-23b and miR-27b transcription during fetal distress and increases the sensitivity of neurons to hypoxia-induced apoptosis [J]. PLoS One, 2015, 10(3): e0120217.
[17] WANG Peng, LIANG Jia, LI Yun, et al. Downregulation of miRNA-30a alleviates cerebral ischemic injury through enhancing beclin 1-mediated autophagy[J]. Neurochemical Research, 2014, 39(7): 1279-1291.

相似文献/References:

[1]伏彩霞a,马宝山,鲁晓波b.缺血性卒中患者血清SOD,NO,MDA水平动态变化与焦虑症发生的相关性[J].现代检验医学杂志,2018,33(06):46.[doi:10.3969/j.issn.1671-7414.2018.06.012]
　FU Cai-xiaa,MA Bao-shan,LU Xiao-bob.Correlation of Dynamic Changes of Serum SOD,NO and MDA in Patients with Ischemic Stroke and Anxiety[J].Journal of Modern Laboratory Medicine,2018,33(05):46.[doi:10.3969/j.issn.1671-7414.2018.06.012]
[2]毕芳芳,史 敏,程微微,等.黄芪甲苷对缺血性卒中小鼠神经功能保护作用及机制研究[J].现代检验医学杂志,2023,38(01):89.[doi:10.3969/j.issn.1671-7414.2023.01.017]
　BI Fang-fang,SHI Min,CHENG Wei-wei,et al.Study on the Protective Effect and Mechanism of Astragaloside IV on Neurological Function in Mice with Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2023,38(05):89.[doi:10.3969/j.issn.1671-7414.2023.01.017]

备注/Memo

备注/Memo:
作者简介：樊春荔（1990-），女，硕士研究生，检验技师，研究方向：miRNA 与心脑血管疾病，E-mail：1204631218@qq.com。
更新日期/Last Update: 2020-10-30