[1]林俊伟,章钧,侯红瑛,等.尿干化学法和离心沉渣镜检法检测穿刺羊水母体细胞污染的应用[J].现代检验医学杂志,2020,35(05):90-92.[doi:10.3969/j.issn.1671-7414.2020.05.023]
　LIN Jun-wei,ZHANG Jun,HOU Hong-ying,et al.Application of Detecting Maternal Cell Contamination in Amniocentesis with Drop-Stick Test and Centrifugal Sediment Microscopy[J].Journal of Modern Laboratory Medicine,2020,35(05):90-92.[doi:10.3969/j.issn.1671-7414.2020.05.023]
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尿干化学法和离心沉渣镜检法检测穿刺羊水母体细胞污染的应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年05期
页码:
90-92
栏目:
论著
出版日期:
2020-11-01

文章信息/Info

Title:
Application of Detecting Maternal Cell Contamination in Amniocentesis with Drop-Stick Test and Centrifugal Sediment Microscopy
文章编号:
1671-7414（2020）05-090-03
作者:
林俊伟; 章钧; 侯红瑛; 叶燕绸; 詹龙生; 刘城
中山大学附属第三医院妇产科，广州 510630
Author(s):
LIN Jun-wei;  ZHANG Jun;  HOU Hong-ying;  YE Yan-chou;  ZHAN Long-sheng;  LIU Cheng
Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Sun Yat - Sen University， Guangzhou 510630，China
关键词:
尿干化学法; 离心沉渣镜检法 ; 母体细胞污染
分类号:
R446.19
DOI:
10.3969/j.issn.1671-7414.2020.05.023
文献标志码:
A
摘要:
目的　探讨一种快速简便鉴别羊水母血污染的方案。方法　选取30 例2019 年6~10 月羊水穿刺样本, 检测 羊水及母血DNA，将母血DNA 污染量换算对应体积全血量建立羊水母血DNA 污染梯度模型，运用尿干化学法及离 心沉渣镜检法检测母血污染情况。结果　羊水母血DNA 污染率为5%，10%，20% 和30%，尿干化学法检出率分别为 16.67%，60%，100% 和100%；离心沉渣镜检法检出率分别为60%，100%，100% 和100%。结论　尿干化学法及离心 沉渣镜检法联合检测可更简便快捷地进行羊水母血污染鉴定，检出率与母血DNA 污染率呈正比关系。
Abstract:
Objective　To establish a quick and simple method to identify the maternal cell contamination in amniotic fluid. Methods　30 samples of amniotic fluid puncture samples from June to October 2019 were selected to detect the amniotic fluid and maternal blood DNA. The contamination amount of maternal blood DNA was converted into the corresponding volume and total blood volume to establish the gradient model of amniotic fluid blood DNA contamination. The Drop-stick test method and centrifugal sediment microscopy were used to detect the contamination of maternal blood. Results　The contamination rate of maternal DNA was 5%, 10%, 20% and 30%, and the detection rate of drop-stick test was 16.67%, 60%, 100% and 100%. The detection rate of centrifuge sediment microscopy was 60%, 100%, 100% and 100%. Conclusion　Combined detection of dropstick test and sediment microscopy can more easily and quickly identify the maternal cell contamination in amniotic fluid.The detection rate is in direct proportion to the DNA contamination rate of mother blood.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介：林俊伟(1986-)，男，本科，主管技师，主要从事细胞遗传工作，E-mail:152464268@qq.com
更新日期/Last Update: 2020-10-30