[1]赵芳芳,郭 红,陈 嘉.LncRNA TUG1 在甲状腺癌组织中的表达及其对细胞增殖和迁移的影响[J].现代检验医学杂志,2020,35(06):42-47.[doi:doi:10.3969/j.issn.1671-7414.2020.06.011]
 ZHAO Fang-fang,GUO Hong,CHEN Jia.Expression of LncRNA TUG1 in Thyroid Carcinoma Tissues and Its Effect onCell Proliferation and Migration[J].Journal of Modern Laboratory Medicine,2020,35(06):42-47.[doi:doi:10.3969/j.issn.1671-7414.2020.06.011]
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LncRNA TUG1 在甲状腺癌组织中的表达及其对细胞增殖和迁移的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年06期
页码:
42-47
栏目:
论著
出版日期:
2020-12-30

文章信息/Info

Title:
Expression of LncRNA TUG1 in Thyroid Carcinoma Tissues and Its Effect onCell Proliferation and Migration
文章编号:
1671-7414(2020)06-042-06
作者:
赵芳芳郭 红陈 嘉
(汉中市人民医院内分泌科,陕西汉中 723000)
Author(s):
ZHAO Fang-fangGUO HongCHEN Jia
(Department of Endocrinology,the People’s Hospital of Hanzhong City,Shaanxi Hanzhong 723000,China)
关键词:
LncRNA TUG1甲状腺癌生物学行为
分类号:
R736.1;R730.43
DOI:
doi:10.3969/j.issn.1671-7414.2020.06.011
文献标志码:
A
摘要:
目的 检测 LncRNA TUG1 在甲状腺癌组织及细胞中的表达,探究其对细胞增殖和迁移的影响。方法 收集 2015年 1 月 ~2018 年 6 月在汉中市人民医院行手术治疗并经病理确诊的 36 例甲状腺癌患者癌组织标本及其相对应的正常癌旁甲状腺组织进行研究。采用 qRT-PCR 法检测甲状腺癌组织及 SW1736,FTC-133 细胞株中 LncRNA TUG1 表达水平。将shRNA 插入慢病毒质粒中构建 LncRNA TUG1 低表达质粒(LV-shTUG1)。采用 MTT 法、集落形成实验及 Transwell 实验检测敲降 LncRNA TUG1 表达对甲状腺癌细胞增殖和迁移的影响;采用蛋白印迹分析实验检测 LncRNA TUG1 对 EMT 相关蛋白E-cadherin和N-cadherin表达的影响。结果 与正常癌旁甲状腺组织相比,甲状腺癌组织中LncRNA TUG1高表达,差异有统计学意义(6.90±1.19 vs 1.51±1.02,t=20.634,P < 0.000)。与 Nthy-ori 3-1 细胞相比,FTC-133 细胞(1.00±0.09vs 4.61±0.15)和 SW1736 细胞(1.00±0.09 vs 2.59±0.23)中 LncRNA TUG1 水平明显升高,差异有统计学意义(t=35.744,P < 0.000;t=11.150,P < 0.000)。敲降 LncRNA TUG1 表达后, SW1736 细胞(1.31±0.19 vs 0.89±0.16)和 FTC-133细胞(2.17±0.09 vs 1.68±0.05)增殖能力较对照组明显降低,克隆形成数目明显减少,差异有统计学意义(t=2.929,P=0.021;t=8.243,P=0.001)。敲降 LncRNA TUG1 表达后,FTC-133 细胞迁移能力(1 675.2±64.2 vs 898.1±156.4)较对照组显著降低,差异有统计学意义(t=7.961,P=0.001)。敲降 LncRNA TUG1 表达后,与对照组相比,E-cadherin 表达量(0.74±0.06 vs 1.66±0.17)显著升高,N-cadherin表达量(1.27±0.18 vs 0.39±0. 07)显著降低,差异有统计学意义(t=8.839,P < 0.000;t=7.892,P=0.001)。结论 LncRNA TUG1 在甲状腺癌组织及细胞中高表达,促进甲状腺癌细胞增殖和迁移,可能通过促进 EMT 的形成进而促进甲状腺癌细胞的生物学行为。

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更新日期/Last Update: 2020-12-20