[1]林李娜a,何　微a,刘国栋b,等.变应性鼻炎患儿血清IL-33，ST2水平及IL-33基因rs3939286G/A位点多态性与疾病程度相关性分析[J].现代检验医学杂志,2022,37(03):127-131.[doi:10.3969/j.issn.1671-7414.2021.03.027]
　LIN Li-naa,HE Weia,LIU Guo-dongb,et al.Correlation Analysis between the Levels of IL-33,ST2 and Polymorphism of IL-33 Gene rs3939286 G/A and Different Degree of Allergic Rhinitis in Children[J].Journal of Modern Laboratory Medicine,2022,37(03):127-131.[doi:10.3969/j.issn.1671-7414.2021.03.027]
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变应性鼻炎患儿血清IL-33，ST2水平及IL-33基因rs3939286G/A位点多态性与疾病程度相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年03期
页码:
127-131
栏目:
论　著
出版日期:
2022-05-15

文章信息/Info

Title:
Correlation Analysis between the Levels of IL-33,ST2 and Polymorphism of IL-33 Gene rs3939286 G/A and Different Degree of Allergic Rhinitis in Children
文章编号:
1671-7414（2022）03-127-05
作者:
林李娜a; 何　微a; 刘国栋b; 凌秀婷b; 王小青a; 梁玉玲a; 王晶晶a; 梁丕凤a
（深圳市龙华区人民医院 a. 全科门诊；b. 检验科，广东深圳 518109）
Author(s):
LIN Li-naa;  HE Weia;  LIU Guo-dongb;  LING Xiu-tingb;  WANG Xiao-qinga;  LIANG Yu-linga; WANG Jing-jinga;  LIANG Pi-fenga
（a. Department of General Clinic; b. Department of Clinical Laboratory,Shenzhen Longhua District People’s Hospital, Guangdong Shenzhen 518109, China）
关键词:
白细胞介素-33; 特异性受体肿瘤发生抑制蛋白2; 单核苷酸多态性; 变应性鼻炎
分类号:
R765.21；R392.11
DOI:
10.3969/j.issn.1671-7414.2021.03.027
文献标志码:
A
摘要:
目的　分析变应性鼻炎（ allergic rhinitis，AR）患儿血清白细胞介素 -33（interleukin-33，IL-33）、特异性受体肿瘤发生抑制蛋白 2（speci.c receptor suppression of tumorigenicity 2 ，ST2）水平及 IL-33基因 rs3939286 G/A位点单核苷酸多态性（ single nucleotide polymorphism，SNP）与疾病程度的相关性。方法　收集 2020年 3月～ 2021年 6月深圳市龙华区人民医院 AR确诊患儿 135例作为 AR组，体检健康儿童 116例作为对照组。分别检测血清 IL-33和 ST2水平，同时对 IL-33基因 rs3939286 G/A位点 SNP进行分析。结果　AR组 IL-33和 ST2水平（ 251.67±39.15 pg/ml， 5.23±0.29 pg/ml）明显高于对照组（ 108.24±20.63 pg/ml，2.06±0.11 pg/ml），差异有统计学意义（ t=7.341 6，8.019 7，均 P <0.05）。中 -重度 AR患儿 IL-33和 ST2水平（ 374.95±58.32 pg/ml，7.98±0.54pg/ml）明显高于轻度 AR（164.28±30.37 pg/ml，3.28±0.17 pg/ml），差异有统计学意义（ t=7.6027， 8.3150，均 P <0.05）。AR组 IL-33基因 rs3939286 G/A位点 AA基因型和 A等位基因频率（ 55.56%，70.74%）明显高于对照组（ 24.14%，38.36%），差异有统计学意义（ χ2= 6.218 4，5.309 2，均 P <0.05）。中 -重度 AR患儿 IL-33基因 rs3939286 G/A位点 AA基因型和 A等位基因频率（ 73.42%，84.18%）明显高于轻度 AR患儿（ 44.65%，62.50%），差异有统计学意义（ χ2=4.902 3，3.981 4，均 P <0.05）。AA基因型 AR患儿血清 IL-33水平（ 303.12±41.60 pg/ml）明显高于 AG基因型（ 214.38±33.15pg/ml），而 AG基因型明显高于 GG基因型（ 129.06±23.78pg/ml），不同基因型差异有统计学意义（ χ2=3.505 4，P=0.039 4）。结论　AR患儿 IL-33和 ST2水平明显升高，与疾病程度有关。同时 AR患儿 IL-33基因 rs3939286 G/A位点存在多态性，不同基因型 IL-33水平存在明显差异，其中 AA基因型 IL-33水平最高，与 AR患儿发病存在一定相关性。
Abstract:
Objective　To analyze the correlation of interleukin-33 (IL-33) and specific receptor suppression of tumorigenicity 2 (ST2) levels, single nucleotide polymorphism (SNP) of IL-33 gene rs3939286 G/A locus and allergic rhinitis (AR) in children with different degrees. Methods　135 children with AR in Shenzhen Longhua District People’s Hospital from March 2020 to June 2021 were selected as AR group, and 116 healthy children were selected as control group. The levels of IL-33 and ST2 in serum were detected, and SNP of IL-33 gene rs3939286 G/A locus was analyzed. Results　The levels of IL-33 and ST2 in AR group (251.67±39.15pg/ml,5.23±0.29 pg/ml) were significantly higher than (108.24±20.63pg/ml, 2.06±0.11pg/ml) in control group, the differences were statistically significant (t=7.3416, 8.0197, all P <0.05). The levels of IL-33 and ST2 in moderatesevere AR children (374.95±58.32pg/ml,7.98±0.54pg/ml) were significantly higher than (164.28±30.37pg/ml,3.28±0.17pg/ ml) in mild AR children, and the differences were statistically significant (t=7.6027, 8.3150,all P < 0.05). The AA genotype and A allele frequency of IL-33 gene rs3939286 G/A locus in AR group (55.56%, 70.74%) were significantly higher than (24.14% , 38.36%) in control group,the differences were statistically significant (χ2=6.2184, 5.3092, all P< 0.05). The AA genotype and A allele frequency (73.42% , 84.18%) of IL-33 gene rs3939286 G/A locus in children with moderate to severe AR were significantly higher than in children with mild AR(44.65%, 62.50%), and the differences were statistically significant (χ2=4.902 3, 3.981 4, all P < 0.05). The level of IL-33 in AR children with AA genotype (303.12±41.60pg/ml) was significantly higher than that of AG genotype (214.38±33.15pg/ml),while AG genotype was significantly higher than that of GG genotype (129.06±23.78pg/ml),and there were significant differences among different genotypes (χ2=3.5054, P=0.039 4). Conclusion　 The levels of IL-33 and ST2 in AR children are significantly increased, which was related to the severity of the disease. At the same time,there was polymorphism in IL-33 gene rs3939286 G/A locus in AR children,and there were significant differences in IL-33 levels among different genotypes, among which AA genotype had the highest IL-33 level, which was correlated with the incidence of AR.

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备注/Memo

备注/Memo:
作者简介：林李娜（1986-），女，本科，主治医师，主要从事临床全科疾病的诊疗工作，E-mail:LLN529679756@163.com。
更新日期/Last Update: 1900-01-01