[1]白 雪,董巧云,赵 丽,等.帕金森病患者血清miR-7 和α-Syn 表达水平与认知功能障碍的相关性研究[J].现代检验医学杂志,2023,38(04):78-82.[doi:10.3969/j.issn.1671-7414.2023.04.014]
　BAI Xue,DONG Qiaoyun,ZHAO Li,et al.Correlation between Serum miR-7 and α-Syn Expression Levels and Cognitive Impairment in Patients with Parkinson’s Disease[J].Journal of Modern Laboratory Medicine,2023,38(04):78-82.[doi:10.3969/j.issn.1671-7414.2023.04.014]
点击复制

帕金森病患者血清miR-7 和α-Syn 表达水平与认知功能障碍的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年04期
页码:
78-82
栏目:
论著
出版日期:
2023-07-15

文章信息/Info

Title:
Correlation between Serum miR-7 and α-Syn Expression Levels and Cognitive Impairment in Patients with Parkinson’s Disease
文章编号:
1671-7414（2023）04-078-05
作者:
白 雪; 董巧云; 赵 丽; 姚 彦; 王 博
（沧州市中心医院神经内五科，河北沧州 061000）
Author(s):
BAI Xue; DONG Qiaoyun; ZHAO Li; YAO Yan; WANG Bo
（Department of Neurology, Cangzhou Central Hospital, Hebei Cangzhou 061000, China）
关键词:
帕金森病; 微小核糖核酸-7; α- 突触核蛋白; 认知功能障碍
分类号:
R742.5；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.04.014
文献标志码:
A
摘要:
目的　通过检测帕金森病（Parkinson's disease，PD）患者血清微小核糖核酸（miRNA，miR）-7 和α- 突触核蛋白（α-synuclein，α-Syn）水平，分析两者与认知功能障碍的关系。方法　收集2020 年1 月～ 2022 年1 月在沧州市中心医院就诊的PD 患者106 例作为PD 组，并根据Hoehn-Yahr 分级标准分为六个等级，随机选择同期性别、年龄相匹配的106 例健康体检者作为对照组。采用qRT-PCR 法检测两组血清miR-7 水平，双抗夹心酶联免疫吸附试验（enzymelinked immunosorbent assay，ELISA）法检测血清α-Syn 水平；采用简易精神状态量表（mini-mental state examination，MMSE）和蒙特利尔认知评估量表（montreal cognitive assessment，MoCA）评估PD 患者认知功能情况；采用Spearman法分析血清miR-7，α-Syn 水平与MMSE 评分、MoCA 评分的相关性，采用Pearson 法分析PD 患者血清miR-7 与α-Syn水平的相关性。结果　PD 组血清miR-7（6.95±1.74）水平低于对照组（8.21±1.35），血清α-Syn（6.28±1.05 μg/L）水平高于对照组（3.52±0.56 μg/L），差异有统计学意义（t=5.890，23.879，均P ＜ 0.05）。PD 患者Hoehn-Yahr 1 级、2 级、2.5 级、3 级、4 级和5 级血清miR-7（8.03±0.99，7.65±0.76，7.12±1.06，6.64±0.65，6.31±0.73，5.93±0.82）水平逐渐降低，α-Syn（3.79±0.39，4.29±0.81，5.48±1.07，6.37±0.82，7.55±1.34，9.01±0.76 μg/L）水平逐渐升高，差异均有统计学意义（F=13.201，55.330，均P ＜ 0.05）。Spearman 法分析显示，PD 患者血清miR-7 水平和MMSE 评分、MoCA 评分均呈正相关（r=0.574，0.597，均P ＜ 0.05）；血清α-Syn 水平和MMSE 评分、MoCA 评分均呈负相关（r=-0.550，-0.537，均P ＜ 0.05）。Pearson 法分析显示，PD 患者血清miR-7 与α-Syn 水平呈负相关（r=-0.608，P ＜ 0.05）。结论　随着PD 患者病情加重，血清miR-7 水平明显降低，α-Syn 水平明显升高，且与患者认知功能障碍密切相关。
Abstract:
Objective　To detect the levels of serum miRNA (miR) -7 and α-synuclein (α-Syn) in patients with Parkinson’s disease (PD), and analyze the relationship between the two and cognitive dysfunction. Methods　106 patients with PD in Cangzhou Central Hospital from January 2020 to January 2022 were selected as PD group, and they were divided into six grades according to Hoehn-Yahr grading standard, 106 healthy people matched with gender and age in the same period were randomly selected as the control group. The qRT-PCR method to detect serum miR-7 levels in the two groups, double resistant sandwich enzyme linked immunosorbent assay (ELISA) method to detect serum α-Syn levels, and the mini-mental state examination (MMSE) and montreal cognitive assessment (MoCA) was used to assess the cognitive function of PD patients. Spearman method was used to analyze the correlation between serum miR-7, α-Syn level and MMSE score, MoCA score, Pearson method was used to analyze the correlation between serum miR-7 and α-Syn level in PD patients. Results　The level of serum miR-7 (6.95±1.74) in the PD group was lower than that of the control group (8.21±1.35), and the level of serum α-Syn (6.28±1.05 μg/L) was higher than that of the control group (3.52±0.56 μg/L), the difference was statistically significant (t=5.890, 23.879, all P<0.05). The level of serum miR-7 (8.03±0.99，7.65±0.76，7.12±1.06，6.64±0.65，6.31±0.73，5.93±0.82) in Hoehn-Yahr grade 1， 2，2.5，3，4 and 5 patients with PD gradually decreased, and the level of α-Syn (3.79±0.39，4.29±0.81， 5.48±1.07，6.37±0.82，7.55±1.34，9.01±0.76 μg/L) gradually increased, with a statistically significant difference (F=13.201,55.330, all P<0.05). Spearman analysis showed that the serum miR-7 level in PD patients was positively correlated with MMSE score and MoCA score (r=0.574, 0.597, all P<0.05), while the serum α-Syn level was negatively correlated with MMSE score and MoCA score (r=-0.550, -0.537, all P<0.05). Pearson analysis showed that there was a negative correlation between serum miR-7 and α-Syn levels in PD patients (r=-0.608，P<0.05). Conclusion　With the aggravation of PD patients, the level of serum miR-7 was significantly decreased, and the level of α-Syn was significantly increased, which was closely related to cognitive dysfunction.

参考文献/References:

[1] SIMON D K, TANNER C M, BRUNDIN P. Parkinson disease epidemiology, pathology, genetics, and pathophysiology[J]. Clinics in Geriatric Medicine, 2020, 36(1): 1-12.
[2] 赵晋英, 李艳, 李艳伟. 帕金森病黑质多巴胺神经元易损伤性的内在因素[J]. 中国生物化学与分子生物学报, 2020, 36(1): 42-48. ZHAO Jinying, LI Yan, LI Yanwei. Inherent factors of dopaminergic neurons vulnerability in the substantia nigra of Parkinson’s disease[J]. Chinese Journal of Biochemistry and Molecular Biology, 2020, 36(1): 42-48.
[3] DU Xiaoyu, XIE Xixiu, LIU Ruitian. The role of α-Synuclein oligomers in Parkinson’s disease[J]. International Journal of Molecular Sciences, 2020, 21(22): 8645.
[4] SHEN Dongfang, QI Huiping, MA Chi, et al. Astaxanthin suppresses endoplasmic reticulum stress and protects against neuron damage in Parkinson’s disease by regulating miR-7/SNCA axis[J]. Neuroscience Research, 2021, 165: 51-60.
[5] 中华医学会神经病学分会帕金森病及运动障碍学组. 中国帕金森病治疗指南（第三版）[J]. 中华神经科杂志, 2014, 47(6): 428-433． Chinese Society of Parkinson’s Disease and Movement Disorders. Chinese guidelines for the treatment of Parkinson’s disease (third edition)[J]. Chinese Journal of Neurology, 2014, 47(6): 428-433.
[6] 中国老年保健协会阿尔茨海默病分会, 解放军总医院国家老年疾病临床医学研究中心. 阿尔茨海默病与帕金森病步态分析的中国专家共识[J]. 中华老年心脑血管病杂志, 2021, 23(11):1141-1145. Alzheimer’s Disease Committea of Chinese Aging Well Association, National Clinical Medical Research Center for Geriatric Diseases, Chinese PLA General Hospital Chinese PLA Medical School. Chinese expert consensus on gait analysis of Alzheimer’s disease and Parkinson’s disease[J]. Chinese Journal of Geriatric Heart Brain and Vessel Diseases, 2021, 23(11): 1141-1145.
[7] 李杰, 谢秀娟, 宋炜, 等．帕金森病患者血浆增强α-突触核蛋白细胞毒性及其机制研究[J]. 首都医科大学学报, 2019, 40(2): 244-250. LI Jie, XIE Xiujuan, SONG Wei, et al. Blood plasma of patients with Parkinson’s disease increases alphasynuclein neurotoxicity and its mechanisms[J]. Journal of Capital Medical University, 2019, 40(2): 244-250.
[8] 安俊言, 安思训, 王鹏. 帕金森病患者血清polo 样激酶2 活性升高促进α- 突触核蛋白聚集[J]. 吉林医药学院学报, 2019, 40(3): 171-174. AN Junyan, AN Sixun, WANG Peng. Increasing the activity of serum polo-like kinase 2 accelerate alphasynuclein aggregation in Parkinson’s patients[J]. Journal of Jilin Medical College, 2019, 40(3): 171-174.
[9] CONTE C. Possible link between SARS-CoV-2 infection and Parkinson’s disease: the role of tolllike receptor 4[J]. International Journal of Molecular Sciences, 2021, 22(13): 7135.
[10] PAN Lina, LI Chunrui, MENG Lanxia, et al. Tau accelerates α-synuclein aggregation and spreading in Parkinson’s disease[J]. Brain, 2022, 145(10): 3454-3471.
[11] MEHRA S, SAHAY S, MAJI S K. α-Synuclein misfolding and aggregation: implications in parkinson’s disease pathogenesis[J]. Biochim Biophys Acta Proteins Proteom, 2019, 1867(10): 890-908.
[12] 杨清, 李大光, 陈勃, 等．帕金森病患者血清α-SYN，LP O 和MDA水平及临床意义[J]．中国实验诊断学,2018, 22（8）：1369-1370． YANG Qing, LI Daguang, CHEN Bo, et al. The levels and clinical significance of serum α-SYN,LPO and MDA in patients with Parkinson’s disease[J]. Chinese Journal of Laboratory Diagnosis, 2018, 22(8): 1369-1370.
[13] 宋启晗, 李旭冉, 李昕, 等. 不同亚型的帕金森病及多系统萎缩患者血浆α- 突触核蛋白寡聚体浓度分析[J]. 首都医科大学学报, 2020, 41(2): 212-216. SONG Qihan, LI Xuran, LI Xin, et al. Differential α-synuclein oligomerization in plasma of patients with different subtypes of Parkinson’s disease and multiple system atrophy[J]. Journal of Capital Medical University, 2020, 41(2): 212-216.
[14] 钟晓鹏, 周永刚, 邱甜, 等．微小RNA 在帕金森病发病机制中的研究进展[J]．赣南医学院学报, 2019,39(12): 1254-1257. ZHONG Xiaopeng, ZHOU Yonggang, QIU Tian, et al. Research progress of microRNA in pathogenesis of Parkinson’s disease[J]. Journal of Gannan Medical University, 2019, 39(12): 1254-1257.
[15] 李群英, 甄时建, 何树光, 等．帕金森病患者治疗期间血清miR-153,miR-128b 水平表达与非运动症状的相关性研究[J]. 现代检验医学杂志, 2021, 36(5):120-123, 168． LI Qunying, ZHEN Shijian, HE Shuguang, et al. Research on correlation between expression of serum miR-153, miR-128b levels and non-motor symptoms in patients with Parkinson’s disease during treatment period[J]. Journal of Modern Laboratory Medicine, 2021, 36(5): 120-123, 168.
[16] 王一帆, 田甜, 闫伟广, 等．miR-7 和miR-153 抑制α-Syn 表达在百草枯诱导多巴胺能神经元损伤中的作用机制[J]. 毒理学杂志, 2021, 35(2): 152-158. WANG Yifan, TIAN Tian, YAN Weiguang, et al. The mechanism of miR-7 and miR-153 inhibiting α-Syn expression in paraquat induced dopaminergic neuronal injury[J]. Journal of Toxicology, 2021, 35(2): 152-158.
[17] TITZE-DE-A LMEIDA R. TITZE-DE-ALMEIDA S S.MiR-7 replacement therapy in Parkinson’s disease[J]. Current Gene Therapy, 2018, 18(3): 143-153.
[18] CHOI D C, YOO M, KABARIA S, et al. MicroRNA-7 facilitates the degradation of alpha-synuclein and its aggregates by promoting autophagy [J]. Neurosci Lett, 2018, 678: 118-123.

相似文献/References:

[1]武 琪a,陈 亮b,张宝华c.帕金森病患者血清和脑脊液褪黑素水平变化及其临床意义[J].现代检验医学杂志,2017,32(05):90.[doi:10.3969/j.issn.1671-7414.2017.05.017]
　WU Qia,CHEN Liangb,ZHANG Bao-huac.Changes and Clinical Significance of Melatonin in Blood and Cerebrospinal Fluid of Patients with Parkinson's Disease[J].Journal of Modern Laboratory Medicine,2017,32(04):90.[doi:10.3969/j.issn.1671-7414.2017.05.017]
[2]武 琪a,张宝华b.Nrf2基因启动子-653G/A,-651G/A和-617C/A位点单核苷酸多态性与帕金森病易感性的关联性研究[J].现代检验医学杂志,2017,32(06):60.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　WU Qia,ZHANG Bao-huab.Association between Nrf2 Gene -653G/A,-651G/A and -617C/A Polymorphism and Susceptibility of Parkinson's Disease[J].Journal of Modern Laboratory Medicine,2017,32(04):60.[doi:10.3969/j.issn.1671-7414.2017.06.001]
[3]李泽东,詹　贞,刘　忆,等.帕金森病患者血清鳞状细胞癌抗原（SCC-Ag）表达的临床意义[J].现代检验医学杂志,2019,34(06):70.[doi:10.3969 ／ j.issn.1671-7414.2019.06.017]
　LI Ze-dong,ZHAN Zhen,LIU Yi,et al.Significance of SCC Antigen Expression in Parkinson’s Disease[J].Journal of Modern Laboratory Medicine,2019,34(04):70.[doi:10.3969 ／ j.issn.1671-7414.2019.06.017]
[4]尚天明,毕开湘.唾液 α- 突触核蛋白 (SNCA) 与嗅觉评估在帕金森病早期筛查中的应用研究[J].现代检验医学杂志,2020,35(06):95.[doi:doi:10.3969/j.issn.1671-7414.2020.06.023]
　SHANG Tian-ming,BI Kai-xiang.Application Research of Olfactory Assessment and Salivary SNCA in EarlyScreening of Parkinson’s Disease[J].Journal of Modern Laboratory Medicine,2020,35(04):95.[doi:doi:10.3969/j.issn.1671-7414.2020.06.023]
[5]李群英,甄时建,何树光,等.帕金森病患者治疗期间血清miR-153，miR-128b水平表达与非运动症状的相关性研究[J].现代检验医学杂志,2021,36(05):120.[doi:10.3969/j.issn.1671-7414.2021.05.027]
　LI Qun-ying,ZHEN Shi-jian,HE Shu-guang,et al.Research on Correlation between Expression of Serum miR-153, miR-128bLevels and Non-motor Symptoms in Patients with Parkinson’s DiseaseDuring Treatment Period[J].Journal of Modern Laboratory Medicine,2021,36(04):120.[doi:10.3969/j.issn.1671-7414.2021.05.027]
[6]闫　欣,商素亮,李　娜,等.血浆S1P和HDL-C表达水平与帕金森病患者临床症状的相关性研究[J].现代检验医学杂志,2022,37(03):182.[doi:10.3969/j.issn.1671-7414.2022.03.038]
　YAN Xin,SHANG Su-liang,LI Na,et al.Correlation between Plasma S1P, HDL-C Expressions Levels and Clinical Symptoms in Patients with Parkinson’s Disease[J].Journal of Modern Laboratory Medicine,2022,37(04):182.[doi:10.3969/j.issn.1671-7414.2022.03.038]

备注/Memo

备注/Memo:
基金项目：河北省2020 年度医学科学研究课题计划（20200301）：早期帕金森病患者嗅觉与认知障碍的特点及其相关性研究。
作者简介：白雪（1986-），女，硕士，主治医师，研究方向：神经内科，E-mail：xbj381@163.com。
通讯作者：董巧云，主任医师，研究方向：神经内科，E-mail：Dqy-20051048@163.com。
更新日期/Last Update: 2023-07-15