[1]梁延连,邬林枫,唐雄驰,等.健康献血者血浆可溶性糖蛋白A(GPA) 在抗-M 与抗-“Mia”抗体阳性及阴性中表达差异的分析研究[J].现代检验医学杂志,2024,39(01):123-125.[doi:10.3969/j.issn.1671-7414.2024.01.022]
　LIANG Yanlian,WU Linfeng,TANG Xiongchi,et al.Analysis of the Difference of Plasma Soluble Glycoprotein A Expression in Positive and Negative Anti-M and Anti-“Mia” Levels in Healthy Blood Donors[J].Journal of Modern Laboratory Medicine,2024,39(01):123-125.[doi:10.3969/j.issn.1671-7414.2024.01.022]
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健康献血者血浆可溶性糖蛋白A(GPA) 在抗-M 与抗-“Mia”抗体阳性及阴性中表达差异的分析研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年01期
页码:
123-125
栏目:
论著
出版日期:
2024-01-15

文章信息/Info

Title:
Analysis of the Difference of Plasma Soluble Glycoprotein A Expression in Positive and Negative Anti-M and Anti-“Mia” Levels in Healthy Blood Donors
文章编号:
1671-7414（2024）01-123-03
作者:
梁延连1; 邬林枫1; 唐雄驰2; 苏宇清1; 吴 凡1; 梁 爽1; 孙丽艳1
（1. 深圳市血液中心输血医学研究所，广东深圳 518040；2. 广西平南县人民医院，广西平南 537300）
Author(s):
LIANG Yanlian1;  WU Linfeng1;  TANG Xiongchi2;  SU Yuqing1;  WU Fan1;  LIANG Shuang1;  SUN Liyan1
（1. Institute of Transfusion Medicine, Shenzhen Blood Center, Guangdong Shenzhen 518040, China; 2. Pinnan County People’s Hospital, Guangxi Pingnan 537300, China）
关键词:
糖蛋白A; MN 血型; 抗-M 抗体; 抗-“Mia”抗体
分类号:
R457.1
DOI:
10.3969/j.issn.1671-7414.2024.01.022
文献标志码:
A
摘要:
目的　分析健康献血者血浆中可溶性糖蛋白A（glycoprotein A，GPA）表达量与抗-M 及抗-“Mia”抗体的相关性。方法　收集2022 年2 月9 日～ 2023 年2 月15 日的健康献血者血浆：不规则抗体阴性的NN型（Ⅰ组，n=118）与MM型（Ⅱ组，n=51），抗-M 抗体阳性的NN 型（Ⅲ组，n=145）与抗-“Mia”抗体阳性的随机型（Ⅳ组，n=87），分别检测4组人不同个体血浆中GPA 的含量，t 检验分析GPA 表达量的差异。结果　Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组血浆中GPA 平均含量分别为：9.941±0.252，10.97±0.256，5.139±0.129 和4.28±0.139ng/ml。Ⅰ组与Ⅱ组的GPA 平均含量较高，Ⅲ组与Ⅳ组的GPA 平均含量较低，显示GPA 平均含量在不规则抗体阴性（Ⅰ组与Ⅱ组）的血浆中较高，在抗-M 与抗-“Mia”抗体阳性（Ⅲ与Ⅳ组）的血浆中较低，差异具有统计学意义（均P ＜ 0.01）。结论　含有抗-M 与抗-“Mia”抗体的健康献血者血浆中GPA 含量明显低于抗体阴性的群体。该研究结果为深入探讨血浆中GPA 是否有中和抗-M 与抗-“Mia”抗体的能力，提高疾病诊断与安全输血打下基础。
Abstract:
Objective　To analyze the correlation between the expression of soluble glycoprotein A (GPA) in plasma of healthy blood donors and anti-M and anti-“Mia” antibodies. Methods　Plasma from healthy donors from February 9，2022 to February 15，2023 was collected: irregular antibody-negative NN type (group Ⅰ，n=118) and MM type (group Ⅱ，n=51)，anti-M antibody positive NN type (group Ⅲ，n=145) and anti-“Mia” antibody positive companion type (group Ⅳ，n= 87)，the GPA content in plasma of different individuals in 4 groups was detected，and the difference in GPA expression was analyzed by t-test. Results　The average plasma GPA contents in groups Ⅰ , Ⅱ , Ⅲ and Ⅳ were 9.941±0.252, 10.97±0.256, 5.139 ±0.129 and 4.28±0.139ng/ml, respectively. The average GPA content of groups Ⅰ and Ⅱ was higher, and the average GPA content of groups Ⅲ and Ⅳ was lower, and the differences were statistically significant（all P ＜ 0.01）. Conclusion　The GPA content in plasma of healthy donors with anti-M and anti-“Mia” antibodies was significantly lower than that of the antibodynegative group. The results of this study lay a foundation for further investigation of whether GPA in plasma has the ability to neutralize anti-M and anti-“Mia” antibodies, improve disease diagnosis and safe blood transfusion.

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备注/Memo

备注/Memo:
基金项目： 深圳市医学三名工程( 项目编号：SZSM201811092)：输血技术学；深圳市医学重点学科（SZXK070）：免疫遗传与输血技术学；广西医疗卫生重点培育学科[ 平南县人民医院消化内科2022（二）：10]：消化系统疾病诊疗。
作者简介： 梁延连（1973-），女，主任技师，研究方向：红细胞血型免疫学与临床输血研究，E-mail:lianviky@126.com。
邬林枫（1986-），男，主管技师，研究方向：输血学检验，E-mail:717083541@qq.com。并列第一作者。
通讯作者：孙丽艳（1994-），女，博士生，研究方向：输血医学，E-mail: Suliyan@hotmail.com。
更新日期/Last Update: 2024-01-15