[1]孔 卓,李春艳.自动化磁珠法提取血清脂溶性维生素应用LC-MS/MS检测的性能评价[J].现代检验医学杂志,2024,39(01):131-135+145.[doi:10.3969/j.issn.1671-7414.2024.01.024]
　KONG Zhuo,LI Chunyan.Performance Evaluation of Automated Magnetic Beads Extraction Method for Extraction of Serum Fat-soluble Vitamins by LC-MS/MS Detection[J].Journal of Modern Laboratory Medicine,2024,39(01):131-135+145.[doi:10.3969/j.issn.1671-7414.2024.01.024]
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自动化磁珠法提取血清脂溶性维生素应用LC-MS/MS检测的性能评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年01期
页码:
131-135+145
栏目:
研究简报·实验技术
出版日期:
2024-01-15

文章信息/Info

Title:
Performance Evaluation of Automated Magnetic Beads Extraction Method for Extraction of Serum Fat-soluble Vitamins by LC-MS/MS Detection
文章编号:
1671-7414（2024）01-131-06
作者:
孔 卓; 李春艳
（北京积水潭医院医学检验中心，北京 100035）
Author(s):
KONG Zhuo;  LI Chunyan
（Department of Clinical Laboratory, Beijing Jishuitan Hospital, Beijing 100035, China）
关键词:
自动化磁珠法; 液相色谱串联质谱; 脂溶性维生素
分类号:
R446.112；Q503
DOI:
10.3969/j.issn.1671-7414.2024.01.024
文献标志码:
A
摘要:
目的　评估自动化磁珠法提取血清脂溶性维生素应用液相色谱串联质谱法（liquid chromatography-tandem massspectrometry，LC-MS/MS）检测的性能。方法　收集200 例临床剩余血清样本，采用自动化磁珠法提取血清中脂溶性维生素A，D2，D3，E 和K；同时联合LC-MS/MS 检测脂溶性维生素A，D2，D3，E 和K 的线性、定量限、精密度、正确度、携带污染率等性能指标以及基质效应。并比较此方法与传统萃取法检测结果的一致性。结果　自动化磁珠法提取脂溶性维生素A，D2，D3，E 和K 线性相关系数均＞ 0.99；五种物质的定量限分别为5，0.25，0.25，125 和0.025ng/ml；批内精密度和批间精密度分别为0.66%~4.83%，0.15%~3.70%；平均加标回收率为87.05%~111.11%；基质效应为95.43%~99.07%；高- 低值样本循环进样结果均值与低- 低值样本循环进样结果均值之差，均小于低- 低值样本循环进样结果均值的3s；统计学结果显示自动化磁珠法和传统萃取法提取的脂溶性维生素结果相关性良好（r ＞ 0.99），两种方法的检测结果无显著偏倚。结论　自动化磁珠法提取脂溶性维生素的检测性能良好，有望提高样品通量和分析效率。
Abstract:
Objective　To evaluate the performance of the automated magnetic beads extraction method for extraction of serum fat-soluble vitamins by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods　A total of 120 clinical residual serum samples were collected automated magnetic beads extraction method was applied to extract serum fatsoluble vitamins A, D2, D3, E and K. The linearity, limit of quantification, precision, accuracy, carryover and matrix effect of fatsoluble vitamins A, D2, D3, E and K were detected by LC-MS/MS. The consistency of the method and conventional extraction method was compared. Results　The linear correlation coefficients for extracting fat-soluble vitamins A, D2, D3, E and K by automated magnetic beads extraction method were all greater than 0.99, and the quantitative limits for these five fat-soluble vitamins were 5, 0.25, 0.25, 125 and 0.025 ng/ml. The intra-batch precision and inter-batch precision were 0.66%~4.83% and 0.15%~3.70%, respectively, and the average spike recovery rate was 87.05%~111.11%. The matrix effect was 95.43%~99.07%, and the difference between the mean value of the cyclic injection results of high-low value samples and the mean value of the cyclic injection results of low-low value samples was less than 3s of the mean value of the cyclic injection results of low-low value samples. The statistical analysis showed that the correlation between the results of the automated magnetic beads extraction method and the conventional extraction method was good (r>0.99), and there was no significant bias in the detection results of the two methods. Conclusion　The automated magnetic beads extraction method for extraction of fat-soluble vitamins had good detection performance, which may be expected to improve sample flux and analysis efficiency.

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备注/Memo

备注/Memo:
基金项目： 国家自然科学基金委员会, 青年科学基金项目（编号：82100257）；北京积水潭医院医工结合项目（编号：YGQ-202311）：质谱检测血清维生素K2 含量预测骨质疏松患者转归临床研究。
作者简介： 孔卓（1980-），男，主治医师，主要研究方向：生化免疫检验，E-mail：q65Zu99@163.com。
通讯作者：李春艳（1988-），女，医师，博士，主要研究方向：质谱方法学的构建和质量评价，E-mail：lcypumc@126.com。
更新日期/Last Update: 2024-01-15