[1]陈 龙,郑详武.建立一种基于BAS化学发光技术检测血清β-hCG，Prog水平的抗生物素干扰方法[J].现代检验医学杂志,2024,39(01):141-145.[doi:10.3969/j.issn.1671-7414.2024.01.026]
　CHEN Long,ZHENG Xiangwu.Establish an Anti-biotin Interference Method to Detect Serum β-hCG and Prog Levels Based on BAS Immunoassays[J].Journal of Modern Laboratory Medicine,2024,39(01):141-145.[doi:10.3969/j.issn.1671-7414.2024.01.026]
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建立一种基于BAS化学发光技术检测血清β-hCG，Prog水平的抗生物素干扰方法()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年01期
页码:
141-145
栏目:
研究简报·实验技术
出版日期:
2024-01-15

文章信息/Info

Title:
Establish an Anti-biotin Interference Method to Detect Serum β-hCG and Prog Levels Based on BAS Immunoassays
文章编号:
1671-7414（2024）01-141-05
作者:
陈 龙1; 郑详武2
（1. 通用环球西安北环医院检验科，西安 710032；2. 山阳县人民医院检验科，陕西商洛 726400）
Author(s):
CHEN Long1;  ZHENG Xiangwu2
（1.Department of Clinical Laboratory，Genertec University Xi’an Bei Huan Hospital，Xi’an 710032，China；2. Department of Clinical Laboratory，Shanyang County People’s Hospital， Shaanxi Shangluo 726400，China）
关键词:
生物素; 链霉亲和素磁珠; 人绒毛膜促性腺激素β 亚基; 孕酮
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2024.01.026
文献标志码:
A
摘要:
目的　在基于生物素- 链霉亲和素（biotin-avidin/streptavidin，BAS）的化学发光技术检测人绒毛膜促性腺激素β 亚基（β-human chorionic gonadotropin，β-hCG）和孕酮（progesterone，Prog）时，建立一种简易、有效的抗生物素干扰方法。方法　采用两种浓度链霉亲和素磁珠（streptavidin coated magnetic micro particles，M）检测不同生物素浓度的高、中、低水平的β-hCG 和Prog 血清，通过回收试验评价两种浓度M 的抗生物素干扰能力以及采用低浓度M 的校准曲线时高浓度M 检测的准确度。结果　① β-hCG 和Prog 的抗生物素干扰能力在低浓度M(0.72 mg/ml) 时分别为100 和25 ng/ml，在高浓度M(1.44 mg/ml) 时分别为500 和50 ng/ml。②使用和低浓度M 相同的校准曲线时，高浓度M对于生物素浓度在500 ng/ml 以下的β-hCG 三个水平的回收率均在90% ～ 110% 之间；对于生物素浓度在50 ng/ml 以下的高、中水平的Prog，其回收率在90% ～ 110% 之间。结论　在基于BAS 化学发光技术检测血清β-hCG 和Prog 时，采用高浓度M（1.44 mg/ml）是一种简易、有效且可靠的抗生物素干扰方法。
Abstract:
Objective　A simple and effective anti-biotin interference method was established to detect human chorionic gonadotropin β subunit (β-hCG) and progesterone (Prog) in BAS immunoassays. Methods　Using two different concentrations of streptavidin magnetic particles（M） to detect high, medium and low levels of β-hCG and Prog serum with different biotin concentrations, the anti-biotin interference ability of two kinds of M and the accuracy of high concentration M to detect β-hCG and Prog were evaluated through recovery test when the calibration curve of low concentration M is adopted. Results　① The anti-biotin interference ability of β-hCG and Prog were 100 and 25 ng/ml respectively at low concentration M(0.72 mg/ml), and were 500 and 50 ng/ml respectively at high concentration M(1.44 mg/ml) . ② When using the same calibration curve as low concentration M, the recovery rate of high concentration M for β-hCG at three levels with biotin below 500 ng/ml were between 90% and 110%，for Prog with high and medium levels of biotin below 50 ng/ml, the recovery rate were between 90% ～ 110%. Conclusion　When detecting serum nterference ability of β-hCG mmunoassays, the method of high concentration M(1.44 mg/ mL) is a simple, effective and reliable anti-biotin interference program.

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备注/Memo

备注/Memo:
作者简介：陈龙（1978-），男，本科，副主任检验技师，主要从事临床生化和免疫学检验，E-mail：52837797@qq.com。
通讯作者：郑详武（1982-），男，本科，副主任检验技师，主要从事临床生化检验，E-mail：1065689897@qq.com。
更新日期/Last Update: 2024-01-15