[1]顾刘雷a,顾 培b,金广浩a,等.鼻咽癌组织中LncRNA CTBP1-AS2 和miR-140-5p 水平表达与放疗疗效及预后的关系研究[J].现代检验医学杂志,2024,39(02):23-27+156.[doi:10.3969/j.issn.1671-7414.2024.02.005]
　GU Liuleia,GU Peib,JIN Guanghaoa,et al.Relationship between the Expression of LncRNA CTBP1-AS2 and miR-140-5p Levels in Nasopharyngeal Carcinoma Tissues and Radiotherapeutic Effect and Prognosis[J].Journal of Modern Laboratory Medicine,2024,39(02):23-27+156.[doi:10.3969/j.issn.1671-7414.2024.02.005]
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鼻咽癌组织中LncRNA CTBP1-AS2 和miR-140-5p 水平表达与放疗疗效及预后的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年02期
页码:
23-27+156
栏目:
论著
出版日期:
2024-03-31

文章信息/Info

Title:
Relationship between the Expression of LncRNA CTBP1-AS2 and miR-140-5p Levels in Nasopharyngeal Carcinoma Tissues and Radiotherapeutic Effect and Prognosis
文章编号:
1671-7414（2024）02-023-06
作者:
顾刘雷a; 顾 培b; 金广浩a; 田 野a; 陶 勇a
（南通市肿瘤医院 a.ICU；b. 放疗科，江苏南通 226006）
Author(s):
GU Liuleia;  GU Peib;  JIN Guanghaoa;  TIAN Yea;  TAO Yonga
(a. ICU; b. Department of Radiotherapy, Nantong Cancer Hospital, Jiangsu Nantong 226006, China)
关键词:
鼻咽癌; 长链非编码RNA 羧基末端结合蛋白1 反义RNA2; 微小核糖核酸-140-5p; 放疗疗效
分类号:
R739.63；R730.43
DOI:
10.3969/j.issn.1671-7414.2024.02.005
文献标志码:
A
摘要:
目的　探讨鼻咽癌癌组织中长链非编码RNA 羧基末端结合蛋白1 反义RNA2（long non-coding RNA C-terminalbinding protein 1 antisense RNA2，LncRNA CTBP1-AS2）、微小核糖核酸（microRNA，miR）-140-5p 水平表达与放疗疗效及预后的关系。方法　选取2018 年3 月～ 2020 年3 月于南通市肿瘤医院确诊的222 例鼻咽癌患者为鼻咽癌组，记录患者临床资料，评估放疗疗效及预后，并分为生存组（n=194）和死亡组（n=28）；另选取同期219 例鼻咽炎患者为对照组。采用Pearson 相关分析检验鼻咽癌患者中LncRNA CTBP1-AS2 与miR-140-5p 表达水平的相关性；采用Kaplan-Meier 生存曲线分析鼻咽癌组织中LncRNA CTBP1-AS2，miR-140-5p 表达水平与患者预后的关系；采用COX 比例风险回归模型对鼻咽癌患者预后进行多因素分析。结果　鼻咽癌组患者组织中LncRNA CTBP1-AS2 表达水平（2.25 ± 0.46）高于对照组（1.02 ± 0.22），miR-140-5p 表达水平（0.67 ± 0.19）低于对照组（1.01 ± 0.23），差异具有统计学意义（t=35.742，16.934，均P ＜ 0.001）。鼻咽癌患者中LncRNA CTBP1-AS2 与miR-140-5p 表达水平呈负相关（r=-0.624，P ＜ 0.001）。LncRNA CTBP1-AS2 高表达的鼻咽癌患者放疗后总有效率（74.11%）和三年生存率（77.68%）低于低表达患者（93.64%，97.27%），差异具有统计学意义（χ2=15.578，19.331，均P ＜ 0.001）；miR-140-5p 高表达患者放疗后总有效率（93.58%）和三年生存率（96.33%）显著高于低表达患者（74.34%，78.76%），差异具有统计学意义（χ2=15.119，15.538，均P ＜ 0.001）。死亡组磁共振酰胺质子转移（amide proton transfer，APT）值（2.10 ± 0.26）、放疗无效（85.71%）、LncRNA CTBP1-AS2 高表达（89.29%）及miR-140-5p 低表达（85.71%）患者比例高于生存组（1.82± 0.31，6.19%，44.85%，45.88%），差异具有统计学意义（t/χ2=4.551，108.127，19.331，15.538，均P ＜ 0.001）。LncRNA CTBP1-AS2 表达水平为鼻咽癌患者三年内死亡的危险因素（HR=2.762，95%CI：1.510 ～ 5.051，P=0.001），miR-140-5p 表达水平为鼻咽癌患者三年内死亡的保护因素（HR=0.817，95%CI：0.718 ～ 0.930，P=0.002）。结论　鼻咽癌组织中LncRNA CTBP1-AS2 高表达，miR-140-5p 低表达，二者与放疗疗效及预后关系密切。
Abstract:
Objective　To investigate the relationship between the expression of long non-coding RNA C-terminal binding protein 1 antisense RNA2 (LncRNA CTBP1-AS2) and microRNA-140-5p (miR-140-5p) levels in nasopharyngeal carcinoma tissues and the radiotherapeutic effect and prognosis. Methods　A total of 222 nasopharyngeal carcinoma patients diagnosed in Nantong Cancer Hospital from March 2018 to March 2020 were collected as the nasopharyngeal carcinoma group. The clinical data of these patients were recorded, the radiotherapeutic effect and prognosis were evaluated, and they were grouped into the survival group（n=194） and the death group（n=28）. Meanwhile, another 219 patients with nasopharyngeal inflammation were collected as the control group. Correlation between LncRNA CTBP1-AS2 and miR-140-5p expression levels in nasopharyngeal carcinoma patients was calculated using Pearson correlation analysis. Kaplan-Meier survival curve was applied to analyze the relationship between the expression levels of LncRNA CTBP1-AS2 and miR-140-5p in nasopharyngeal carcinoma tissue and prognosis. Multivariate analysis was conducted on the prognosis of nasopharyngeal carcinoma patients using Cox proportional risk regression model. Results　The expression level of LncRNA CTBP1-AS2 in the tissues of patients in nasopharyngeal carcinoma group (2.25 ± 0.46) was higher than that in the control group (1.02 ± 0.22), while the expression level of miR-140-5p (0.67 ± 0.19) was lower than that in the control group (1.01 ± 0.23), and the differences were statistically significant (t=35.742, 16.934, all P<0.001). There was a negative correlation between LncRNA CTBP1-AS2 and miR-140-5p expression levels in nasopharyngeal carcinoma patients (r=-0.624, P<0.001). The total effective rate (74.11%) and 3-year survival rate (77.68%) of nasopharyngeal carcinoma patients with high expression of LncRNA CTBP1-AS2 after radiotherapy were lower than those with low expression (93.64%, 97.27%), and the differences were statistically significant (χ2=15.578, 19.331, all P<0.001). The total effective rate (93.58%) and 3-year survival rate (96.33%) of patients with high expression of miR- 140-5p after radiotherapy were higher than those of patients with low expression (74.34%, 78.76%), and the differences were statistically significant (χ2=15.119, 15.538, all P<0.001). The magnetic resonance amide proton transfer (APT) value (2.10 ± 0.26), the proportion of patients with radiotherapy failure (85.71%), high expression of LncRNA CTBP1-AS2 (89.29%), and low expression of miR-140-5p (85.71%) in the death group were higher than those in the survival group (1.82 ± 0.31, 6.19%, 44.85%, 45.88%), and the differences were statistically significant (t/χ2=4.551, 108.127, 19.331, 15.538, all P<0.001). The level of LncRNA CTBP1-AS2 was a risk factor for mortality within 3 years in nasopharyngeal carcinoma patients (HR=2.762, 95%CI: 1.510 ～ 5.051, P=0.001), while the level of miR-140-5p was a protective factor for mortality within 3 years in nasopharyngeal carcinoma patients (HR=0.817, 95%CI: 0.718 ～ 0.930, P=0.002). Conclusion　LncRNA CTBP1-AS2 was highly expressed, while miR-140-5p was lowly expressed in nasopharyngeal carcinoma tissue, indicating the two may be closely related to the radiotherapeutic effect and prognosis.

参考文献/References:

[1] 潘国刚, 杨碧秀. 鼻咽癌不同临床分期中白细胞介素水平表达的最新研究进展[J]. 现代检验医学杂志,2021, 36(5): 184-188. PAN Guogang, YANG Bixiu. Latest research progress on the expression mechanism of interleukin in nasopharyngeal carcinoma different clinical staging[J]. Journal of Modern Laboratory Medicine, 2021, 36(5): 184-188.
[2] LUAN Weihong, YUAN Haozhan, HOU Wei, et al. Improvement and prognosis analysis of nimotuzumab combined with TP regimen induction chemotherapy and sequential concurrent chemoradiotherapy in patients with locally advanced nasopharyngeal carcinoma[J].American Journal of Translational Research, 2022,14(8): 5630-5640.
[3] TANG Linglong, GUO Rui, ZHANG Ning, et al. Effect of radiotherapy alone vs radiotherapy with concurrent chemoradiotherapy on survival without disease relapse in patients with low-risk nasopharyngeal carcinoma:a randomized clinical trial[J]. Journal of the American Medical Association, 2022, 328(8): 728-736.
[4] HUANG Bo, LI Yiliang, DENG Zhuoxia, et al. Regulatory mechanism of LncRNA CTBP1-AS2 in nasopharyngeal carcinoma cell proliferation and apoptosis via the miR-140-5p/BMP2 axis[J]. Protein and Peptide Letters, 2022, 29(7): 621-630.
[5] YOU Rui, ZOU Xiong, WANG Shunlan, et al. New surgical staging system for patients with recurrent nasopharyngeal carcinoma based on the AJCC/UICC rTNM classification system[J]. European Journal of Cancer, 2015, 51(13): 1771-1779.
[6] EISENHAUER E A, THERASSE P, BOGAERTS J, et al. New response evaluation criteria in solid tumours:revised RECIST guideline (version 1.1)[J]. European Journal of Cancer, 2009, 45(2): 228-247.
[7] ZHONG Guihua, WEI Wei, LIAO Wei, et al. Tumor microbiome in nasopharyngeal carcinoma and its association with prognosis[J]. Frontiers in Oncology,2022, 12: 859721.
[8] 薛玉峰, 张会平, 王芳, 等. 鼻咽癌患者血清miR-144-3p 及miR-151-3p 的表达水平及临床价值[J]. 现代检验医学杂志, 2021, 36(5): 65-68. XUE Yufeng, ZHANG Huiping, WANG Fang, et al. Expression and clinical value of serum miR-144-3p and miR-151-3p levels in patients with nasopharyngeal carcinoma[J]. Journal of Modern Laboratory Medicine,2021, 36(5): 65-68.
[9] AI Q H, HUNG K F, SO T Y, et al. Prognostic value of cervical nodal necrosis on staging imaging of nasopharyngeal carcinoma in era of intensity-modulated radiotherapy: a systematic review and meta-analysis[J].Cancer Imaging, 2022, 22(1): 24.
[10] NG W T, CHOW J C H, BEITLER J J, et al. Current radiotherapy considerations for nasopharyngeal carcinoma[J]. Cancers, 2022, 14(23): 5773.
[11] WANG Haihua, WANG Weiyuan, FAN Songqing. Emerging roles of LncRNA in nasopharyngeal carcinoma and therapeutic opportunit ies [ J ] . International Journal of Biological Sciences, 2022,18(7): 2714-2728.
[12] GUO Zhen, WANG Youhong, XU Heng, et al. LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma[J]. Cell Death & Disease, 2021, 12(1): 69.
[13] WANG Weifeng, ZHANG Zhuo, LI Yundong, et al. Down-regulated long non-coding RNA LHFPL3 antisense RNA 1 inhibits the radiotherapy resistance of nasopharyngeal carcinoma via modulating microRNA-143-5p/homeobox A6 axis[J]. Bioengineered, 2022,13(3): 5421-5433.
[14] LI Yongfeng, ZONG Jin, ZHAO Cong. LncRNA CTBP1-AS2 promotes proliferation and migration of glioma by modulating miR-370-3p-Wnt7a-mediated epithelial-mesenchymal transition[J]. Biochemistry and Cell Biology, 2020, 98(6): 661-668.
[15] LIU Lixia, LIU Bin, YU Jie, et al. SP1-induced upregulation of LncRNA CTBP1-AS2 accelerates the hepatocellular carcinoma tumorigenesis through targeting CEP55 via sponging miR-195-5p[J]. Biochemical and Biophysical Research Communications, 2020, 533(4):779-785.
[16] LE T A H, LAO T D. Circulating microRNAs as the potential diagnostic and prognostic biomarkers for nasopharyngeal carcinoma[J]. Genes, 2022, 13(7):1160.
[17] TIAN Yutong, TANG Lu, YI Pin, et al. MiRNAs in radiotherapy resistance of nasopharyngeal carcinoma[J].Journal of Cancer, 2020, 11(13): 3976-3985.
[18] SONG Qingxu, CHEN Pengxiang, CHEN Xin, et al. Dynamic change of amide proton transfer imaging in irradiated nasopharyngeal carcinoma and related histopathological mechanism[J]. Molecular Imaging and Biology, 2021, 23(6): 846-853.
[19] 叶裕丰, 许宗为, 曾伟华, 等. 酰胺质子转移成像技术在鼻咽癌放化疗后疗效预测的价值[J]. 磁共振成像, 2020, 11(8) ：635-640. YE Yufeng, XU Zongwei, ZENG Weihua, et al. The value of amide proton transfer imaging in predicting the effect of nasopharyngeal carcinoma after chemoradiotherapy[J]. Chinese Journal of Magnetic Resonance Imaging, 2020, 11(8): 635-640.

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备注/Memo

备注/Memo:
基金项目： 2022 年度南通市卫生健康委科研课题( 指导性) 立项项目（QNZ2022026）：酰胺质子转移成像（APT）技术在鼻咽癌放疗靶区勾画中的应用及近期疗效评价研究。
作者简介：顾刘雷（1992-），女，硕士，住院医师，研究方向：急危重症患病人的救治， E-mail：gll0111@sina.cn。
通讯作者：陶勇（1976-），男，硕士，副主任医师，研究方向：急危重症患病人的救治， E-mail：1036922438@qq.com。
更新日期/Last Update: 2024-03-15