[1]周 欣,黄翠兰.聚合酶链反应方法检测阴道毛滴虫准确性的meta 分析[J].现代检验医学杂志,2024,39(02):168-174.[doi:10.3969/j.issn.1671-7414.2024.02.031]
　ZHOU Xin,HUANG Cuilan.Accuracy of Polymerase Chain Reaction Based Assays for Trichomonas Vaginitis: A Meta-Analysis[J].Journal of Modern Laboratory Medicine,2024,39(02):168-174.[doi:10.3969/j.issn.1671-7414.2024.02.031]
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聚合酶链反应方法检测阴道毛滴虫准确性的meta 分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年02期
页码:
168-174
栏目:
论著
出版日期:
2024-03-31

文章信息/Info

Title:
Accuracy of Polymerase Chain Reaction Based Assays for Trichomonas Vaginitis: A Meta-Analysis
文章编号:
1671-7414（2024）02-168-07
作者:
周 欣; 黄翠兰
（岳池县人民医院妇产科，四川广安 638300）
Author(s):
ZHOU Xin; HUANG Cuilan
（Department of Obstetrics and Gynecology, the People’s Hospital of Yuechi County, Sichuan Guangan 638300, China）
关键词:
阴道毛滴虫; 聚合酶链反应; meta 分析
分类号:
R382.21；Q503
DOI:
10.3969/j.issn.1671-7414.2024.02.031
文献标志码:
A
摘要:
目的　系统评价聚合酶链反应检测阴道毛滴虫的应用价值。方法　 检索中国知网数据库(CNKI)、万方数据知识服务平台、维普数据库和PubMed 数据库自建库到2023 年5 月30 日以来基于PCR 方法检测阴道毛滴虫的研究文献。经文献筛选、数据提取后，使用软件RevMan 5.4.1 和网页服务Meta-Disc 2.0 进行Meta 分析。结果　共纳入36 篇文献，16 454 个临床样本。Meta 分析结果显示：聚合酶链反应检测阴道毛滴虫的合并敏感度为0.972（0.943，0.987），合并特异度为0.979（0.968，0.986），诊断比值比为1 643.398（673.168，4 012.008），阳性似然比为46.209（30.549，69.897）和阴性似然比为0.028（0.013，0.059）；亚组分析表明不同性别的临床样本不会影响聚合酶链反应的检测准确性，但不同的PCR 检测方法的准确性有差异。结论　聚合酶链反应检测阴道毛滴虫的准确性较高，具有良好的应用价值。
Abstract:
Objective　To investigate the accuracy of polymerase chain reaction ( PCR ) based assays for Trichomonas Vaginitis. Methods　The research literature on the detection of TV based on PCR method since the establishment of the database to May 30，2023 was retrieved from China National Knowledge Infrastructure ( CNKI ), Wanfang Data Knowledge Service Platform, VIP Database and PubMed Database. After literature screening and data extraction, Meta-analysis was performed using software RevMan 5.4.1 and web service Meta-Disc 2.0. Results　A total of 36 literatures and 16 454 clinical samples were included. The results of meta-analysis showed that the combined sensitivity and specificity of polymerase chain reaction for the detection of TV were 0.972 (0.943, 0.987 ) and 0.979 ( 0.968, 0.986 ), respectively, the diagnostic odds ratio was 1 643.398 ( 673.168, 4 012.008 ), the positive likelihood ratio was 46.209 ( 30.549, 69.897 ), and the negative likelihood ratio was 0.028 ( 0.013, 0.059 ). Subgroup analysis showed that clinical samples of different genders would not affect the accuracy of polymerase chain reaction, but the accuracy of different PCR detection methods was different. Conclusion　The accuracy of polymerase chain reaction in the detection of TV was high and it has good application value.

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备注/Memo

备注/Memo:
作者简介：周欣（1993-），女，学士，住院医师，主要从事妇产科疾病防治，E-mail：17781759380@163.com。
更新日期/Last Update: 2024-03-15