[1]唐 湘,王 莉,韩泽平,等.Apollon siRNA抑制胰腺癌SW1990细胞增殖的研究[J].现代检验医学杂志,2015,30(01):27-30.[doi:10.3969/j.issn.1671-7414.2015.01.008]
　TANG Xiang,WANG Li,HAN Ze-ping,et al.Study on Proliferation of Pancreatic Cancer SW1990 Cell Lines by Small Interfering RNA Targeted Apollon[J].Journal of Modern Laboratory Medicine,2015,30(01):27-30.[doi:10.3969/j.issn.1671-7414.2015.01.008]
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Apollon siRNA抑制胰腺癌SW1990细胞增殖的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
27-30
栏目:
论著
出版日期:
2015-01-30

文章信息/Info

Title:
Study on Proliferation of Pancreatic Cancer SW1990 Cell Lines by Small Interfering RNA Targeted Apollon
文章编号:
1671-7414(2015)01-027-05
作者:
唐 湘1; 王 莉2; 韩泽平2; 何金花2; 李宝霞3
1.广州市番禺区新造医院检验科,广州 511436;
2.广州市番禺区中心医院检验科,广州 511400;
广州市暨南大学医学院生化教研室,广州 510632
Author(s):
TANG Xiang1; WANG Li2; HAN Ze-ping2; HE Jin-hua2; LI Bao-xia3
1.Department of Clinical Laboratory,Panyu Xinzao Hospital of Guangzhou City, Guangzhou 511400,China;
2.Department of Clinical Laboratory, Panyu Center Hospital of Guangzhou City,Guangzhou 511400,China;
3.Department of Biochemistry,Jinan Univers
关键词:
Apollon;  凋亡抑制蛋白;  小干扰RNA;  胰腺癌;  SW1990细胞;  增殖
分类号:
R735.9; R730.43
DOI:
10.3969/j.issn.1671-7414.2015.01.008
文献标志码:
A
摘要:
目的 研究凋亡抑制蛋白(inhibitor of apoptosis proteins,IAPs)家族成员Apollon小干扰RNA(small interference RNA,siRNA)对人胰腺癌SW1990细胞增殖的影响,并进一步探讨其作用机制。方法 将前期研究的Apollon siRNA的序列,经脂质体包裹转染胰腺癌(SW1990)细胞48 h后,采用WST-8法检测Apollon siRNA对胰腺癌SW1990细胞增殖抑制作用,并计算IC50。流式细胞术检测细胞早期凋亡率; RT-PCR检测Apollon mRNA的相对表达水平,western-blotting检测Apollon蛋白的表达水平。结果 Apollon siRNA作用于胰腺癌(SW1990)细胞48 h后,能有效抑制SW1990细胞增殖,并呈现剂量效应关系; 流式细胞术检测结果显示:ApollonsiRNA能促进胰腺癌(SW1990)细胞凋亡,其早期凋亡率达37.1%; RT-PCR结果显示,Apollon siRNA能显著下调Apollon mRNA及蛋白在胰腺癌细胞中的表达水平。结论 Apollon siRNA可抑制胰腺癌细胞的增殖,作用机制可能为共同促进胰腺细胞早期凋亡、下调mRNA及蛋白的表达有关,为临床上胰腺癌的靶向基因治疗提供理论基础。
Abstract:
Objective To study the effect of small interfering RNA targeted on Apollon for proliferation on pancreatic cancer cells and itspossible acting mechanism.Methods The small interferingRNA targeted on apollon in our previous study was transfected to the cells using LipofectamineTM 2000,after 48 hours transtection.The inhibitory effects of small interfering RNA targeted on Apollon(Apollon siRNA)on cell proliferation were detected by WST-8.Their inhibition rate and IC50 were calculated.The percentage of apoptosis cells were determined by flow cytometry.The expression of Apollon mRNA was analyzed by real time fluorescent quantitative reverse transcription polymerase chain reaction.The Apollon protein expression levels weredetected by western blotting.Results Apollon siRNA could effectively inhibit the proliferation of pancreatic cancer cell.The amount ofapoptotic cells increased significantly.The early apoptotic rate was 37.1%,and the RT-PCR results showed that the relative expression levels of Apollon mRNA were down-regulate,and shows a dose-effectiveness relations.The protein expression levels were decreased by Apollon siRNA.Conclusion Apollon siRNA can effectively inhibit the proliferation of pancreatic cancercell.The mechanism may be work together to promote pancreative cancer cell early apoptosis and decreased the expression levels of gene and protein,which provides a novel potential approach for treatment of target therapy of pancreatic cancer.

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备注/Memo

备注/Memo:
基金项目:广东省中医药管理局项目(2014)。
作者简介:唐 湘(1973-),男,本科,主管技师,从事医学检验工作,研究方向:靶向基因治疗,Tel:13663630086,E-mail:captan918@126.com。
通讯作者:何金花,E-mail:332518579@qq.com。
更新日期/Last Update: 2015-01-30