[1]严孝岭,刘国瑞,虞 伟,等.体液免疫9项指标总误差与不确定度比较分析[J].现代检验医学杂志,2015,30(01):55-57.[doi:10.3969/j.issn.1671-7414.2015.01.015]
　YAN Xiao-ling,LIU Guo-rui,YU Wei,et al.Comparison Research Between Total Error and Uncertainty in Nine Humoral Immune Items[J].Journal of Modern Laboratory Medicine,2015,30(01):55-57.[doi:10.3969/j.issn.1671-7414.2015.01.015]
点击复制

体液免疫9项指标总误差与不确定度比较分析()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
55-57
栏目:
论著
出版日期:
2015-01-30

文章信息/Info

Title:
Comparison Research Between Total Error and Uncertainty in Nine Humoral Immune Items
文章编号:
1671-7414(2015)01-055-04
作者:
严孝岭; 刘国瑞; 虞 伟; 李晓军
南京大学医学院附属金陵医院,南京军区南京总医院中心实验科,南京210002
Author(s):
YAN Xiao-ling; LIU Guo-rui; YU Wei; LI Xiao-jun
Jinling Hospital,Medical School of Nanjing University Nanjing General Hospital of Nanjing Command,Nanjing 210002,China
关键词:
总误差;  不确定度;  体液免疫
分类号:
R531.3; R381
DOI:
10.3969/j.issn.1671-7414.2015.01.015
文献标志码:
A
摘要:
目的 利用总误差(TE)和不确定度的统计方法评价体液免疫项目的检测质量。方法 利用伯乐质量控制软件计算2013年1月～10月金陵医院中心实验科体液免疫项目室内质量控制累积均值((-overx)),累积标准差(s),累积变异系数(CV),利用中国卫生部临床检验中心室间质评回报数据[共2次(201311～201315,201321～201325)]计算体液免疫项目检测结果与靶值的相对偏倚(bias%)和相对平均偏倚(bias%^-),结合室内质量控制累积CV计算TE。使用室间质评回报数据和室内质量控制累积CV,采用Nordtest法计算每个项目的不确定度。结果 2个质量控制水平的免疫球蛋白G(IgG)、免疫球蛋白A(IgA)、免疫球蛋白M(IgM)、补体3(C3)、补体4(C4)、抗链球菌溶血素“O”(ASO)、类风湿因子(RF)、C反应蛋白(CRP)和免疫球蛋白E(IgE)的最大TE分别为:13.07%,14.05%,13.99%,15.68%,14.84%,14.5%,16.55%,14.19%和10.05%; 这些项目的最大扩展不确定度分别为:15.44%,15.82%,16.23%,17.67%,16.73%,19.96%,19.01%,16.25%和10.86%。IgG,IgA,IgM,C3的不确定度构成比中偏倚和不精密度所占比例大致相同,而C4,CRP,IgE的不确定度主要受不精密度影响。ASO,RF的不确定度主要受偏倚影响。结论 TE和不确定度之间有一致性,可作为实验室检测质量分析和改进的重要依据,不确定度可提供更多参考价值。
Abstract:
Objective To evaluate the performance of humoral immune items by the statistical method of total error(TE)and uncertainty.Methods The data of internal quality control were collected by Bio-Rad quality controllsoftware from January to October 2013,and the accumulation means((-overx)),accumulation standard deviations(s)and accumulation coefficients of variation(CV)were computed.According to the external quality assessment plans from Chinese National Center for Clinical Laboratories
[2 reports(201311～201315,201321～201325)].The relative bias(bias%)and relative average bias(bias%^-)of humoral immune item results and target values were calculated.TE was calculated with the accumulation coefficients of variation and the relative bias.By Nordtest method,the uncertainties of humoral immune items werecalculated according to the external quality assessment data and internal quality control accumulation CV.Results The maximum TEs with every 2 internal quality control levels of immunoglobulin G(IgG),immunoglobulin A(IgA),immunoglobulin M(IgM),complement 3(C3),complement 4(C4),antistreptolysin “O”(ASO),rheumatoid factor(RF),C-reactive protein(CRP),immunoglobulin E(IgE)were 13.07,14.05,13.99,15.68,14.84,14.,16.55,14.19,10.05.The uncertainties of those items were 15.44,15.82,16.23,17.67,16.73,19.96,19.01,16.25,10.86.In the constituent ratioof the uncertainties,imprecision play major roles in items of C4,CRP,IgE while bias play major roles in items of ASO,RF.The two elements approach parity initems of IgG,IgA,IgM,C3.Conclusion Both TE and uncertainty have good consistency,and they can be the important reference for the analysis and improvement of laboratory quality.But uncertainty can provide more informations for reference.

参考文献/References:

[1] ISO.Guide to the expression of uncertainty in measurement[S].Geneva:International Organization for Standardization Corrected and Reprinted,1995.
[2] Magnusson B,Naykki T,Hovind H,et al.Handbook for calculation of measurement uncertainty in environmental laboratories(edition 2)[R].Finland:Nordtest,2004.
[3] 王治国.临床检验质量控制技术[M].北京:人民卫生出版社,2008:412-432. Wang ZG.Clinical examination quality control[M].Beijing:People's Medical Publishing House,2008:412-432.
[4] 刘文彬,居 漪.常规化学项目总误差和不确定度比较研究[J].检验医学,2012,27(12):1002-1006. Liu WB,Ju Y.Comparison research between total error and uncertainty in routine clinical chemistry items[J].Laboratory Medicine,2012,27(12):1002-1006.
[5] 吴 斌,李一松,贺 勇,等.25项临床生物化学指标测量不确定度的评估[J].临床检验杂志,2012,30(5):378-379. Wu B,Li YS,He Y,et al.Assessment of the measurement uncertaintyin 25 clinical biochemistry items[J].Chin J Clin Lab Sci,2012,30(5):378-379.
[6] 梁敏文,郑有为,邸玉玮,等.临床化学定量项目测量的不确定度评估分量的分析[J].中国卫生检验杂志,2012,22(11):2678-2681. Liang MW,Zheng YW,Di YW,et al.Uncertainty evaluation of quantitative clinical biochemistry analysis[J].Chinese Journal of Health LaboratoryTechnology,2012,22(11):2678-2681.
[7] 张 莹,周铁成,岳乔红,等.探讨测量不确定度在临床生化检验中的应用[J].现代检验医学杂志,2012,27(5):92-94. Zhang Y,Zhou TC,Yue QH,et al.Study on the application on measurement ucertainty of clinical biochemisty[J].J Mod Lab Med,2012,27(5):92-94.
[8] 朱红梅,吴美辉,罗 丹,等.临床生化检验测量不确定度的评估[J].检验医学与临床,2012,9(8):922-923. Zhu HM,Wu MH,Luo D,et al.Evaluation of measurement uncertainty in clinical biochemical detection[J].Lab Med Clin,2012,9(8):922-923.
[9] National Pathology Accreditation Advisory Council(NPAAC).Requirements for the estimation of measurement uncertainty[S].Canberra:Commonwealth of Australia,Australian Government Department,2007.

相似文献/References:

[1]张诗诗,王 薇,赵海建,等.临床检验总误差与测量不确定度[J].现代检验医学杂志,2016,31(05):153.[doi:10.3969/j.issn.1671-7414.2016.05.046]
　ZHANG Shi-shi,WANG Wei,ZHAO Hai-jian,et al.Total Error and Measurement Uncertainty in Clinical Laboratory Medicine[J].Journal of Modern Laboratory Medicine,2016,31(01):153.[doi:10.3969/j.issn.1671-7414.2016.05.046]
[2]陈荣贵,唐曙明,刘运红,等.应用 σ度量对深圳市龙华区三家综合医院临床生化检验结果互认的评价研究[J].现代检验医学杂志,2021,36(01):141.[doi:10.3969/j.issn.1671-7414.2021.01.035]
　CHEN Rong-gui,TANG Shu-ming,LIU Yun-hong,et al.Evaluation of Mutual Recognition of Biochemical Results in Three Hospitals of Longhua District by σ Metric[J].Journal of Modern Laboratory Medicine,2021,36(01):141.[doi:10.3969/j.issn.1671-7414.2021.01.035]

备注/Memo

备注/Memo:

作者简介:严孝岭(1983-),男,检验技师,硕士在读,主要研究方向:免疫学诊断,E-mail:yxllxyj@163.com。
通讯作者:李晓军(1962-),女,主任技师,教授,博士生导师,E-mail:lixiaojun62@yahoo.com.cn。
更新日期/Last Update: 2015-01-30