[1]李晓阳,胡春卉a,唐文娟,等.肿瘤坏死因子-α基因启动 子区-308G/A多态性与慢性乙型肝炎的关联性[J].现代检验医学杂志,2015,30(01):58-63.[doi:10.3969/j.issn.1671-7414.2015.01.016]
 LI Xiao-yang,HU Chun-huia,TANG Wen-juan,et al.Correlation between the TNF-α Gene Promoter-308G/A Polymorphism and the Chronic Hepatitis B[J].Journal of Modern Laboratory Medicine,2015,30(01):58-63.[doi:10.3969/j.issn.1671-7414.2015.01.016]
点击复制

肿瘤坏死因子-α基因启动 子区-308G/A多态性与慢性乙型肝炎的关联性()
分享到:

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
58-63
栏目:
论著
出版日期:
2015-01-30

文章信息/Info

Title:
Correlation between the TNF-α Gene Promoter-308G/A Polymorphism and the Chronic Hepatitis B
文章编号:
1671-7414(2015)01-058-06
作者:
李晓阳1胡春卉2a唐文娟3袁 情4易显富2b
1.长沙市泰和医院检验科,长沙 410000;
2.湖北医药学院附属太和医院 a.检验科;
b.急诊科,湖北十堰 442000;
3.十堰市妇幼保健院妇女保健科,湖北十堰 442000;
4.广州中医药大学第一附属医院检验科,广州 510405
Author(s):
LI Xiao-yang1HU Chun-hui2aTANG Wen-juan3YUAN Qing4YI Xian-fu2b
1.Department of Clinical Laboratory,Taihe Hospital in Changsha,Changsha 410000,China;
2a.Department of Clinical Laborator;
2b.EmergencyDepartment, Affiliated Taihe Hospital of Hubei Medical University,Hubei Shiyan 442000,China;
关键词:
肿瘤坏死因子-α -308G/A多态性 慢性乙型肝炎 HBV-DNA
分类号:
R512.62; Q786
DOI:
10.3969/j.issn.1671-7414.2015.01.016
文献标志码:
A
摘要:
目的 研究肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)基因启动子区-308位点G/A多态性与慢性乙型肝炎(chronic hepatitis B,CHB)的关联性。方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测142例慢性乙型肝炎患者(CHB组)和150例健康者(HC组)的TNF-α基因启动子区-308位点基因型,定量检测ALT,AST,HBeAg,HBV-DNA,HBV-LP和HBV-PreS1,并研究其与TNF-α-308G/A各基因型间的相关性。结果 CHB组与HC组比较,TNF-α位点-308位点各基因型及等位基因分布频率差异无统计学意义(P>0.05),A等位基因与CHB无统计学关联(OR=1.529,OR95%CI:0.872~2.684); TNF-α 308G/A多态性与AST,ALT,HBV-LP和HBV-PreS1阳性率无相关性(P>0.05),但与HBeAg和HBV-DNA阳性率存在统计学关联(P<0.05),且A等位基因可增加HBeAg和HBV-DNA阳性表达风险(HBeA:OR=3.256,OR95%CI为1.105~9.594; HBV-DNA:OR=2.847,OR95%CI为1.059~7.655); A与G等位基因比较,CHB组病毒载量在104~107时差异具有统计学意义(P<0.05)。结论 TNF-α 308G/A多态性与CHB易感性无关,但等位基因A与HBV-DNA病毒载量相关,可增加HBV感染风险。
Abstract:
Objective To investigate the association between the single nucleotide polymorphisms(SNP)in-308 loci of tumor necrosis factor-α(TNF-α)gene promoter region and chronic hepatitis B(CHB).Methods Genotypes of -308 loci of the TNF-α promoter were examined by the polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP)in 142 patients with chronic hepatitis B(CHB group)and 150 healthycontrols(HC group).The indexes for evaluating the curative effect were the ALT,AST,HBeAg,HBV-DNA and the viral load weight,HBV-LP and HBV-PreS1,meanwhile,the correlations between related indexes and SNP in TNF-α 308 loci wereexplored as well.Results There was no statistical significance in frequency distribution difference of the genotypes and alleles of -308 loci between CHC and HC groups(P>0.05),the protective factors of TNF-α 308 allele A may be not associated with CHB(OR=1.529,OR95%CI:0.872~2.684).There was no association between TNF-α gene promoter -308G/A polymorphism and the positive rates of AST,ALT,HBV-LP and HBV-PreS1(P>0.05),however,TNF-α-308G/A polymorphism associates with the positive rates of HBeAg and HBV-DNA,and A-allele of 308 loci may increase the risk of HBeAg andHBV-DNA positive expression(HBeAg:OR=3.256,OR95%CI=1.105~9.594; HBV-DNA:OR=2.847,OR95%CI=1.059~7.655).Furthermore,A-allele compared with G-allele,statistically significant differences were observed in the certain HBV-DNAviral load range of 104~107 copies/ml(P<0.05).Conclusion TNF-α gene promoter-308G/A polymorphism would not be associated withCHB,but the TNF-α-308 gene G mutation of A-allele,which was associated with HBV-DNA viral load,may be the susceptible factors of HBV infection.

参考文献/References:

[1] 李智彬,刘永明,苏何玲,等.免疫学应答与慢性乙型肝炎血清学转换的关系[J].中华临床医师杂志(电子版),2013,7(23):10990-10992. Li ZB,Liu YM,Su HL,et al.The relationship between immune response and chronic hepatitis B seroconversion[J].Chinese Journal of Clinicians(Electronic Edition),2013,7(23):10990-10992.
[2] 陈继梅,吕 亮,丁雪芳.慢性乙型肝炎患者血清HBVDNA载量与肝功能指标关系研究[J].中华全科医学,2012,10(4):618-619. Chen JM,Lu L,Ding XF.Association study of serum HBV DNA level andindicators of liver function in patients with chronic Hepatitis B[J].ChineseJournal of General Practice,2012,10(4):618-619.
[3] 黎 环,潘 晨.肿瘤坏死因子α基因-308位点多态性与乙型肝炎病毒慢性感染的关系[J].中华实验和临床感染病杂志(电子版),2010,4(1):4-6. Li H,Pan C.Association of polymorphism of tumor necrosis factor alpha gene promoter region-308 with outcomes of chronic hepatitis B virus infection[J].Chin J Eep Clin Infect Dis(Electronic Edition),2010,4(1):4-6.
[4] 陈 立,陈治新,潘 晨,等.肿瘤坏死因子α基因多态性与慢性乙型肝炎病毒感染的相关性[J].中华肝脏病杂志,2006,14(8):630-631. Chen L,Chen ZX,Pan C,et al.The relationship between tumor necrosis factor alpha gene polymorphism and chronic hepatitis B virus infection[J].Chinese Journal of Hepatology,2006,14(8):630-631.
[5] Zhang TC,Zhao YQ,Hu GL,et al.The relationship between tumour necrosis factor-alpha gene polymorphism and susceptibility and clearance of the persistent hepatitis B virus infection in a Chinese population:a meta-analysis[J].Clin Microbiol Infect,2014,20(3):227-234.
[6] 高海兵,潘 晨,林明华,等.HBeAg阳性慢性乙型肝炎患者肿瘤坏死因子α基因启动子区-238和-308位点基因多态性分析[J].实用肝脏病杂志,2010,13(4):258-259,268. Gao HB,Pan C,Lin MH,et al.Polymorphisms of promoter at-238 and-308 of necrosis factor-α gene in patients with HBeAg positive chronic hepatitis B[J].Journal of Clinical Hepatology,2010,13(4):258-259,268.
[7] 石志平,吴同玉,刘 瑶,等.乙型肝炎肝硬化湿热证与肿瘤坏死因子α基因多态性相关性研究[J].中华中医药杂志,2014,29(6):2004-2006. Shi ZP,Wu TY,Liu Y,et al.Research on the association betwwen hepatitis B liver cirrhosis of dampness-heat syndrome and tumor necrosis factor agene polymorphism[J].Chin J Trad Chin Med Phar,2014,29(6):2004-2006.
[8] 胡春卉,陈 晋,杨 锐,等.MxA基因启动子区-88G/T,-123C/A单核苷酸多态性与HCV易感性和IFN-α疗效评价研究[J].细胞与分子免疫学杂志,2012,28(12):1307-1310. Hu CH,Chen J,Yang R,et al.Association between single nucleotidepolymorphisms in -88 and -123 loci of MxA gene promoter region and HCV susceptibility and IFN-α efficacy[J].Chin J Cell Mol Immunol,2012,28(12):1307-1310.
[9] Kao PC,Wu JF,Ni YH,et al.Tumour necrosis factor-alpha promoter region polymorphisms affect the course of spontaneous HBsAg clearance[J].Liver Int,2010,30(10):1448-1453.
[10] 高海兵,潘 晨.肿瘤坏死因子α与乙型肝炎病毒感染相关性的研究进展[J].中国肝脏病杂志(电子版),2010,2(3):45-50. Gao HB,Pan C.Chinese Journal of liver Diseases(Electronic Version),2010,2(3):45-50.
[11] 宗禹希,陈 萱,吕 楠,等.TNF-α与乙型病毒性肝炎宫内感染的相关性研究[J].中国优生与遗传杂志,2012,20(12):4-6.
[12] 施前锋,王叶萍.HBeAg阴性慢性乙型肝炎患者血清乙型肝炎病毒外膜大蛋白检测分析[J].中华实验和临床病毒学杂志,2009,23(2):135-137. Shi QF,Wang YP.Detecting significance of HBV-LP in the HBeAg negative patients with chronic hepatitis B[J].Chinese Journal of Experimental and Clinical Virology,2009,23(2):135-137.
[13] 张 砾.前S1抗原与乙型肝炎血清标志物检测的相关性及临床意义[J].中国医药指南,2012,10(12):528-529. Zhang S.Guide of China Medicine,2012,10(12):528-529.
[14] Schieck A,Schulze A,Gahler C,et al.Hepatitis B virus hepatotropism is mediated by specific receptor recognition in the liver and not restricted to susceptible hosts[J].Hepatology,2013,58(1):43-53.
[15] 王 琮,徐春军,江 涛.慢性病毒性肝炎肝功能指标辅助判断中医辨证分型浅析[J].中华中医药杂志,2013,28(4):970-972. Wang Z,Xu CJ,Jiang T.A brief analysis of chronic viral hepatitis liverfunction inde guiding TCM syndrome differentiation[J].China Journal of Traditional Chinese and Pharmacy,2013,28(4):970-972.
[16] Forbi JC,Ben-Ayed Y,Xia GL,et al.Disparate distribution of hepatitis B virus genotypes in four sub-Saharan African countries[J].J Clin Virol,2013,58(1):59-66.
[17] Gonzalez S, Rodrigo L, Martinez-Borra J,et al.TN-F-alpha -308A promoter polymorphism is associated with enhanced TNF-alpha production and inflammatory activity in Crohn's patients with fistulizing disease[J].Am J Gastroenterol,2003,98(5):1101-1106.

相似文献/References:

[1]孟凡凡,齐晓林,黄奕江,等.乌司他丁干预对水下爆炸致兔急性肺损伤TNF-α表达的影响[J].现代检验医学杂志,2017,32(03):131.[doi:10.3969/j.issn.1671-7414.2017.03.036]
 MENG Fan-fan,QI Xiao-lin,HUANG Yi-jiang,et al.Effect of Ulinastatin on the Expression of TNF-α in the Rabbits with Acute Lung Injury Induced by Underwater Explosion[J].Journal of Modern Laboratory Medicine,2017,32(01):131.[doi:10.3969/j.issn.1671-7414.2017.03.036]
[2]张 劲,明 媚.增生性糖尿病视网膜病变患者玻璃体、房水和血浆中VEGF表达与IL-6,IL-8和TNF-α水平的相关性研究[J].现代检验医学杂志,2021,36(04):55.[doi:10.3969/j.issn.1671-7414.2021.04.012]
 ZHANG Jin,MING Mei.Correlation of VEGF Expression with IL-6, IL-8 and TNF-α Levels inVitreous Body, Aqueous Humor and Plasma of Patients with ProliferativeDiabetic Retinopathy[J].Journal of Modern Laboratory Medicine,2021,36(01):55.[doi:10.3969/j.issn.1671-7414.2021.04.012]
[3]宋婷阁,张鹏举,耿 彪,等.新生儿急性呼吸窘迫综合征患者血清miR-183-5p的表达及与IL-1β,IL-6和TNF-α水平的相关性[J].现代检验医学杂志,2021,36(05):55.[doi:10.3969/j.issn.1671-7414.2021.05.012]
 SONG Ting-ge,ZHANG Peng-ju,GENG Biao,et al.Expression of Serum miR-183-5p in Patients with Neonatal Acute RespiratoryDistress Syndrome and Its Correlation with IL-1 β, IL-6 and TNF-α[J].Journal of Modern Laboratory Medicine,2021,36(01):55.[doi:10.3969/j.issn.1671-7414.2021.05.012]
[4]姜永滨,任东铭,隋成君.下肢动脉硬化闭塞症患者血清IL-17 和TNF-α 水平表达及其临床意义[J].现代检验医学杂志,2022,37(05):168.[doi:10.3969/j.issn.1671-7414.2022.05.033]
 JIANG Yong-bin,REN Dong-ming,SUI Cheng-jun.Expression and Clinical Significance of Serum Levels of IL-17 and TNF-α in Patients with Arteriosclerosis Obliterans of Lower Extremity[J].Journal of Modern Laboratory Medicine,2022,37(01):168.[doi:10.3969/j.issn.1671-7414.2022.05.033]
[5]刘晓燕,张小佛,李 嘉,等.重症腺病毒肺炎患儿血清肝素结合蛋白和肿瘤坏死因子-α 表达水平及其对疾病预后评估价值研究[J].现代检验医学杂志,2023,38(01):151.[doi:10.3969/j.issn.1671-7414.2023.01.028]
 LIU Xiao-yan,ZHANG Xiao-fo,LI Jia,et al.Study on the Expression Level of Serum Heparin Binding Protein and Tumor Necrosis Factor-α and Its Prognostic Value in Children with Severe Adenovirus Pneumonia[J].Journal of Modern Laboratory Medicine,2023,38(01):151.[doi:10.3969/j.issn.1671-7414.2023.01.028]

备注/Memo

备注/Memo:
基金项目:湖北教育厅资助课题(鄂教科D20122404)。
作者简介:李晓阳(1978-),女,医学硕士,主管检验技师,研究方向:临床免疫与微生物学检验,Tel:0731-88518888-2038,18229706665,E-mail:sahalara2010@163.com。
通讯作者:易显富,E-mail:taihexianhu@163.com。
更新日期/Last Update: 2015-01-30