[1]谭皓妍,冯浩华,何艳屏.阴道白假丝酵母菌的耐药性与MDR1基因表达关系研究[J].现代检验医学杂志,2015,30(01):105-107.[doi:10.3969/j.issn.1671-7414.2015.01.029]
 TAN Hao-yan,FENG Hao-hua,HE Yan-ping.Research the Expression of MDR1 Genes in Vaginal Candida Albicans Resistance to Azole Agents[J].Journal of Modern Laboratory Medicine,2015,30(01):105-107.[doi:10.3969/j.issn.1671-7414.2015.01.029]
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阴道白假丝酵母菌的耐药性与MDR1基因表达关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
105-107
栏目:
论著
出版日期:
2015-01-30

文章信息/Info

Title:
Research the Expression of MDR1 Genes in Vaginal Candida Albicans Resistance to Azole Agents
文章编号:
1671-7414(2015)01-105-04
作者:
谭皓妍冯浩华何艳屏
广州市海珠区沙园街社区卫生服务中心,广州 510250
Author(s):
TAN Hao-yanFENG Hao-huaHE Yan-ping
Shayuan Street Community Health Service of Haizhu District,Guangzhou 510250,China
关键词:
MDR1基因 基因表达 白假丝酵母菌 吡咯类药物 耐药
分类号:
R379.9; Q786
DOI:
10.3969/j.issn.1671-7414.2015.01.029
文献标志码:
A
摘要:
目的 探讨白假丝酵母菌中MDR1基因表达水平与菌株对吡咯类药物敏感性下降的关系。方法 于2011年8月~2012年8月收集反复发作阴道炎患者白假丝酵母菌60株,用科玛嘉念珠菌显色培养基作菌种鉴定,纸片扩散法进行药敏试验; 根据药敏结果把菌株分为4组菌株:全敏感组、氟康唑中度敏感或耐药组、酮康唑中度敏感或耐药组、咪康唑中度敏感或耐药组,每组随机选取12株菌株,运用荧光定量PCR技术检测菌株中MDR1基因的表达量,采用t检验方法把中度敏感或耐药组与敏感组的MDR1基因表达水平进行比较分析。结果 60株白假丝酵母菌中酮康唑中度敏感或耐药株有26株、氟康唑中度敏感或耐药株有12株、咪康唑中度敏感或耐药株有38株、全敏感株有22株; 酮康唑中度敏感或耐药组、氟康唑中度敏感或耐药组、咪康唑中度敏感或耐药组和敏感组中MDR1基因相对表达量分别为3.32±4.46,2.27±3.05,0.9±0.81和0.41±0.47; 酮康唑、氟康唑、咪康唑的中度敏感或耐药组与敏感组比较,t值分别为-2.177,-2.130和-2.094,差异均无统计学意义(P>0.05)。结论MDR1基因的表达量与临床白假丝酵母菌对吡咯类药物的耐药性关系有待进一步研究。
Abstract:
Objective To investigate the expression levelsof MDR1 gene from azole resistant strains in vaginal Candida albicans.Methods 60 strains of Candida albicans from recurrent vagintis patients were collected and identified by CHROM agar from Aug.2011 to Aug.2012.The drug resistance was detected by disk diffusion method.According tothe result of drug sensitivity test,the strains were diveded into four groups:sensitive group moderately susceptible or resistant to fluconazole group moderately susceptible or resistant to detoconazole group and moderately susceptible orresistant to miconazole group.Each group was selected 12 strains randomly.Theexpression of the MDR1 gene in these 48 strains were detected by real-time RT-PCR and analysed the datas by t test statistics.Results There were 22 sensitive strains,26 strains of moderately susceptible or resistant to detoconazole,12 strains of moderately susceptible or resistant to fluconazole and 38 strains of moderately susceptible or resistant to miconazole in 60 strains of Candida albicans.The relative expression of MDR1 gene in sensitive group was 0.41±0.47,in moderately susceptible or resistant to detoconazole group fluconazole group and miconazole group were 3.32±4.46,2.27±3.05 and 0.9±0.81 respectively.Compared with the sensitive group,t values of moderately susceptible or resistant to detoconazole group,fluconazole group and miconazole group were -2.177,-2.130 and -2.094.The expression levelof MDR1 gene had no statistical significance between the sensitive strains and moderately susceptible or resistant strains(P>0.05).ConclutionThe relationship between MDR1 expression and the resistance to azoleagents in vaginal Candida albicans requires further study.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:广州市海珠区2011年科技计划项目(2011-QY-06)。
作者简介:谭皓妍(1977-),女,医学硕士,主管技师,从事医学临床检验的研究和工作,Tel:13660063242,E-mail:13660063242@163.com。
更新日期/Last Update: 2015-01-30