[1]李大鹏,李 震,王 刚,等.创伤性失血性休克大鼠血浆 血栓调节蛋白的动态测定及价值[J].现代检验医学杂志,2015,30(01):116-117.[doi:10.3969/j.issn.1671-7414.2015.01.033]
　LI Da-peng,LI Zhen,WANG Gang,et al.Dynamic Observation and Value of Plasma Level of Thrombomodulin in Rats with Traumatic Hemorrhagic Shock[J].Journal of Modern Laboratory Medicine,2015,30(01):116-117.[doi:10.3969/j.issn.1671-7414.2015.01.033]
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创伤性失血性休克大鼠血浆 血栓调节蛋白的动态测定及价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
116-117
栏目:
研究简报·实验技术
出版日期:
2015-01-30

文章信息/Info

Title:
Dynamic Observation and Value of Plasma Level of Thrombomodulin in Rats with Traumatic Hemorrhagic Shock
文章编号:
1671-7414(2015)01-116-03
作者:
李大鹏1; 李 震2; 王 刚3; 张永军1; 王凤娇1; 郑文哲1
1.解放军88医院,山东泰安 271000;
2.泰安市中心医院,山东泰安 271000;
3.泰安军分区干休四所,山东泰安 271000
Author(s):
LI Da-peng1; LI Zhen2; WANG Gang3; ZHANG Yong-jun1; WANG Feng-jiao1; ZHENG Wen-zhe1
1.the 88th Hospital of PLA,Shandong Taian 271000,China;
2.Taian Center Hospital,Shandong Taian 271000,China;
3.Taian Military Subarea Accept the Four,Shandong Taian 271000,China
关键词:
创伤性;  失血性休克;  动物模型;  血管内皮细胞;  损伤;  血栓调节蛋白
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2015.01.033
文献标志码:
A
摘要:
目的 探讨创伤性失血性休克(traumatic hemorrhagic shock,THS)中血浆血栓调节蛋白(thrombomodulin,TM)动态变化及其临床价值。方法 选择无特定病原体成年大鼠17只,通过急性失血方式建立收缩压下降曲线,形成THS动物模型,测定收缩压基础值(T1),记录收缩压降至基础值3/4(T2)、基础值1/2(T3)、基础值2/5(T4)、基础值1/5(T5),10 mmHg(T6)失血量,采用酶联免疫吸附分析检测各个时间点血浆TM水平。结果 各时间组间存在显著性差异,T2～T3时间段内TM水平随时间延长而升高,T4～T5时间段内TM水平随时间延长而降低,T5～T6时间段内TM水平随时间延长而升高。结论 选择收缩压下降曲线建立THS模型评估失血量变化,结合TM水平动态监测,可对血管内皮细胞损伤程度与THS相关过程进行有效评估。
Abstract:
Objective To explore the dynamic changes in theplasma levels of thrombomodulin(TM)with traumatic hemorrhagic shock(THS).Methods The model of the THS with gradient decerease in thesystolic blood pressure was eatablished in 17 rats without specific pathogen.The arterial blood during the different phase of shock,i.e.when the systolic pressures were three forth,one second,two fifth of the basis systolic blood pressure,one fifth of the basic systolic blood pressure and 10mmHg,was collected and TM levels in the plasma were determined by the double antibody sandwich method of enzyme-labeled immunosorbent assay(ELISA)and analyzed.Results There were significant differences in plasma levels of TM betweengroups,with TM levels in T2～T3 period of time increased with the extension oftime,and TM levels in T4～T5 period of time decreased with the extension of time,and TM levels in T5～T6 period of time increased with the extension of time.Conclusion The animal model of THS was related to the systolic pressure during the shock,may be helpful to assessment changes in blood loss.The dynamic observation of the plasma levels of TM which is a good index ofthe damage to the vascular endothelial cells,may be helpful to assessment of the THS is related to the degree of damage to the vascular endothelial cells.

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备注/Memo

备注/Memo:
基金项目:军队“十二五”后勤科研计划项目(课题编号:CWS115297)。
作者简介:李大鹏(1964-),男,汉族,本科,副主任技师,研究方向:医学实验,Tel:0518-8839734,E-mail:lidapeng1964@126.com。
更新日期/Last Update: 2015-01-30