[1]宫琦玉.miR-9-3在慢性淋巴细胞白血病中的功能与调控机制[J].现代检验医学杂志,2015,30(02):7-10.[doi:10.3969/j.issn.1671-7414.2015.02.003]
　GONG Qi-yu.Function and Regulation of miR-9-3 in Chronic Lymphocytic Leukemia[J].Journal of Modern Laboratory Medicine,2015,30(02):7-10.[doi:10.3969/j.issn.1671-7414.2015.02.003]
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miR-9-3在慢性淋巴细胞白血病中的功能与调控机制()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
7-10
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Function and Regulation of miR-9-3 in Chronic Lymphocytic Leukemia
作者:
宫琦玉
内蒙古牙克石市人民医院检验科,内蒙古牙克石 022150
Author(s):
GONG Qi-yu
Department of Clinical Laboratory, Yakeshi People's Hospital,Inner Mongolia Yakeshi 022150,China
关键词:
慢性淋巴细胞白血病;  miR-9-3;  甲基化特异性聚合酶链反应;  5-氮杂-2'-脱氧胞苷
分类号:
R557.4; R392.11
DOI:
10.3969/j.issn.1671-7414.2015.02.003
文献标志码:
A
摘要:
目的 探讨microRNA-9-3(miR-9-3)在慢性淋巴细胞白血病中的作用及调控机制。方法 采用甲基化特异性聚合酶链反应(MSP)技术检测8例正常人骨髓组织和外周血、78例新确诊慢性淋巴细胞性白血病患者骨髓组织和7种白血病细胞系的甲基化水平; 采用Western blot技术检测甲基化阳性白血病细胞株的NF-κB1信号转导通路活化水平。结果 正常对照组miR-9-3启动子呈非甲基化状态; 7种白血病细胞株中只有I83-E95和WAC3CD5+两种细胞株种呈非甲基化状态(MSP阳性率为28.6%); 78例慢性淋巴细胞性白血病患者中65例发生miR-9-3甲基化(MSP阳性率为83%)。去甲基化药物5-氮杂-2'-脱氧胞苷(5-Aza2'Dc)处理后的I83-E95和WAC3CD5+细胞株miR-9-3均处于去甲基化状态。结论 慢性淋巴细胞性白血病患者存在miR-9-3异常甲基化,可能导致癌细胞异常增生; miR-9-3去甲基化可抑制NF-κB1信号通路活化,表明miR-9-3可能可以通过该通路抑制癌细胞凋亡,引发患者疾病恶化; 甲基化的白血病细胞株可被去甲基化药物抑制,因此miR-9-3可以作为治疗慢性淋巴细胞白血病的新基因靶点。
Abstract:
Objective To investigate the role and regulatory mechanism of micro RNA-9-3(miR-9-3)in the pathogenesis of chronic lymphocytic leukemia.Methods Using the methylation specific PCR(MSP)technology to detect 8 cases of normal bone marrow tissue and peripheralblood,78 cases of bone marrow tissue came from the chronic lymphocytic leukemia pateints newly diagnosed and the methylation level of 7 kinds of leukemia cellline.Used Western blot to detected the NF-kappa B1 signal transduction pathway activation levels of methylation positive leukemia cell line.Results The miR-9-3 of normal control group were in the negative methylation status.Only I83-E95 and WAC3CD5+ were in positive methylation status inseven kinds of leukemia cell line(the positive of MSP was 28.6%); 65 cases occurred miR-9-3 methylated in 78 of chronic lymphocytic leukemia patients(thepositive of MSP was 83%).I83-E95 and miR-9-3 cells of WAC3CD5+ were in themethylation state when treatment with 5-nitrogen-2'-deoxidization cytidine(5-Aza2'Dc).Conclusion The abnormal methylation of miR-9-3 were usually seenin chronic lymphocytic leukemia,it could lead to abnormal hyperplasis in cancer cells.The methylation of miR-9-3 could inhibit the activation of NF-kappa B1 signal pathway suggested that it could suppress the apoptosis of cancer cells through this pathways to trogered the progression of disease.The inhibitor of methylation could be induced the demethylation of leukemia cell lines,so it is possible that miR-9-3 maight be a new gene targets forthe treatment of chronic lymphocytic leukemia.

参考文献/References:

[1] Hagemann T,Wilson J,Kulbe H,et al.Macrophages induce invasiveness of epithelial cancer cells via NF kappa B and JNK[J].J Immunol,2005,175(2):1197-1205.
[2] Calin GA,Croce CM.MicroRNA signatures in human cancers[J].Nat Rev Cancer,2006,6(11):857-866.
[3] Zhang B,Pan X,Cobb GP,et al.MicroRNAs as oncogenes and tumor suppressors[J].Dev Biol,2007,302(1):1-12.
[4] Wang LQ,Liang R,Chim CS.Methylation of tumor suppressor microRNAs:lessons from lymphoid malignancies[J].Expert Rev Mol Diagn,2012,12(7):755-765.
[5] Tsai KW,Liao YL,Wu CW,et al.Aberrant hypermethylation of miR-9 genesin gastric cancer[J].Epigenetics,2011,6(10):1189-1197.
[6] Ma L,Young J,Prabhala H,et,al.MiR-9 a MYC/MYCN-activated microRNA,regulates e-cadherin and cancer metastasis[J].Nat Cell Biol,2010,12(3):247-256.
[7] Esteller M.Epigenetics in cancer[J].N Engl J Med,2008,358(11):1148-1159.
[8] Kanduri,C.Long noncoding RNA and epigenomics[J].Adv Exp Med Bio,2011(722):174-195.
[9] Sharma S,Kelly TK,Jones PA.Epigenetics in cancer[J].Carcinogenesis,2010,31(1):27-36.
[10] Dixon-Mclver A,East P,Mein CA,et al.Distinctive patterns ofmicroRNA expression associated with karyotype in acute myeloid leukaemia[J].PLoS One,2008.3(5):e2141.
[11] Zhang HY,Qi M,Li SW,et al.MicroRNA-9 targets matrix metalloproteinase 14 to inhibit invasion,metastasis,and angiogenesis of neuroblastoma cells[J].Mol Cancer Ther,2012,11(7):1454-1466.
[12] Wan HY, Guo LM, Liu T,et al.Regulation of the transcription factor NF-κ B1 by microRNA-9 in human gastric adenocarcinoma[J].Mol Cancer,2010(9):16.

备注/Memo

备注/Memo:
作者简介:宫琦玉(1968-),女,本科,副主任检验师,从事临床微生物和临床血液病研究,Tel:13644807055。
更新日期/Last Update: 2015-03-20