[1]沈红艳a,邓演超a,徐 湛a,等.2型糖尿病肾病患者外周血IL-17和IL-22 mRNA水平[J].现代检验医学杂志,2015,30(02):30-32,35.[doi:10.3969/j.issn.1671-7414.2015.02.009]
　SHEN Hong-yana,DENG Yan-chaoa,XU Zhana,et al.Levels of IL-17 and IL-22 mRNA in the Blood of Type 2 Diabetic Nephropathy Patients[J].Journal of Modern Laboratory Medicine,2015,30(02):30-32,35.[doi:10.3969/j.issn.1671-7414.2015.02.009]
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2型糖尿病肾病患者外周血IL-17和IL-22 mRNA水平()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
30-32,35
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Levels of IL-17 and IL-22 mRNA in the Blood of Type 2 Diabetic Nephropathy Patients
作者:
沈红艳a; 邓演超a; 徐 湛a; 鹿 璐a; 梁 军b
徐州市中心医院a.检验科; b.内分泌科,江苏徐州 221006
Author(s):
SHEN Hong-yana; DENG Yan-chaoa; XU Zhana; LU Lua; LIANG Junb
a.Department of Clinical Laboratory; b.Department of Endocrinology, Xuzhou Central Hospital,Jiangsu Xuzhou 221006,China
关键词:
白介素-17;  白介素-22;  糖尿病肾病
分类号:
R587.1; R392.11
DOI:
10.3969/j.issn.1671-7414.2015.02.009
文献标志码:
A
摘要:
目的 研究2型糖尿病(T2DM)患者肾脏不同病变时期外周血IL-17和IL-22 mRNA水平,探索糖尿病肾病(DN)患者IL-17和IL-22基因表达水平与肾脏病变的关系。方法 实验包括60名2型T2DM患者和20名正常对照者(NC),其中糖尿病患者包括:无蛋白尿组(NA,n=22)、微量清蛋白尿组(MA,n=18)和糖尿病肾病组(DN,n=20)。采用实时荧光定量PCR方法检测IL-17和IL-22 mRNA水平,分析各组外周血IL-17和IL-22 mRNA水平差异。结果 DN组IL-17和IL-22 mRNA水平显著高于MA组,NA组和NC组差异有统计学意义(P<0.01); MA,NA和NC三组之间IL-17和IL-22 mRNA差异无统计学意义(P>0.05)。结论 在2型糖尿病肾病外周血中IL-17和IL-22 mRNA水平升高,IL-17和IL-22可能在DN病变中发挥作用。
Abstract:
Objective To investigate levels of IL-17 and IL-22 mRNA in the blood of type 2 diabetes(T2DM)patients with the different stages of kidney injury and explore the relationship between the gene expression levels of IL-17,IL-22 and renal lesions in patients with diabetic nephropathy(DM).Methods Subjects included 60 T2DM patients with or without kidney injury and 20 normal controls(NC,n=20).Diabetes patients weredivided into 3 groups by level of urinary albumin to creatinine ratio(ACR):noproteinuria group(NA,ACR<30 mg/g,n=22),microalb uminuria group(MA,30 mg/g>ACR>300 mg/g,n=18)and diabetic nephropathy group(DN,ACR>300 mg/g,n=20).Quantitative Real-Time RT-PCR was used to detect IL-17 and IL-22 mRNA levels.Analysis differences of IL-17 and IL-22 mRNA levels among NA,MA,DN and NC groups.Results The levels of IL-17 andIL-22 mRNA were significantly higher in DN group than that in MA,NA and NC group(P<0.01,respectively),However,there were not significant difference among MA, NA and NC group(P>0.05,respectively).Conclusion Levels IL-17 and IL-22 mRNA were increased in blood of T2DM patientswith nephropathy.IL-17 and IL-22 may play role in the pathogenicity of diabetic nephropathy.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:徐州市中心医院博士(硕士)创新团队科技项目(项目编号:XZS2012090)。 作者简介:沈红艳(1976-),女,硕士,副主任技师,主要研究方向:分子免疫诊断,Tel:0516-85790102,E-mail:jieshyan2006@126.com。 通讯作者:梁 军,硕士生导师,主任医师,E-mail:mwlj521@163.com。
更新日期/Last Update: 2015-03-20