[1]李 璐,梁 辉a,陈群蓉b.鼻咽癌组织中SYK基因启动子区的甲基化分析[J].现代检验医学杂志,2015,30(02):39-41,45.[doi:10.3969/j.issn.1671-7414.2015.02.012]
　LI Lu,LIANG Huia,CHEN Qun-rongb.Methylation of SYK Gene Promoter Region in Nasopharyngeal Carcinoma[J].Journal of Modern Laboratory Medicine,2015,30(02):39-41,45.[doi:10.3969/j.issn.1671-7414.2015.02.012]
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鼻咽癌组织中SYK基因启动子区的甲基化分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
39-41,45
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Methylation of SYK Gene Promoter Region in Nasopharyngeal Carcinoma
作者:
李 璐1; 梁 辉2a; 陈群蓉2b
1.北京大学深圳医院检验科,广东深圳 518035; 2.深圳市宝安区人民医院a.耳鼻咽喉科,b.输血科,广东深圳 518101
Author(s):
LI Lu1; LIANG Hui2a; CHEN Qun-rong2b
1.Department of Clinical Laboratory,Shenzhen Hospital of Peking University,Guangdong Shenzhen 518035,China; 2a.Department of Otorhinolaryngology; 2b.Department of Blood Transfusion,People's Hospital of Shenzhen Baoan,Guangdong Shenzhen 518101,China
关键词:
鼻咽癌;  SYK基因;  启动子;  甲基化
分类号:
R739.6; R730.43
DOI:
10.3969/j.issn.1671-7414.2015.02.012
文献标志码:
A
摘要:
目的 检测鼻咽癌组织中脾酪氨酸激酶(SYK)基因启动子区的甲基化状态,探讨SYK基因启动子区的甲基化与鼻咽癌发生的关系。方法 收集2012年2月～2014年8月间在深圳市宝安区人民医院和北京大学深圳医院就诊的鼻咽癌患者52例,慢性鼻咽黏膜炎症患者26例,鼻咽癌和慢性鼻咽黏膜炎症患者均经病理学诊断证实。应用甲基化特异性PCR技术检测鼻咽癌和慢性鼻咽黏膜炎症组织的SYK基因启动子区的甲基化状态,比较鼻咽癌和慢性鼻咽黏膜炎症组织的SYK基因启动子区的甲基化率。结果 在52例鼻咽癌组织中有11例SYK基因启动子区存在甲基化,甲基化率为21.2%,而在26例慢性鼻咽黏膜炎症组织中未检测到SYK基因启动子区的甲基化。结论 鼻咽癌组织中SYK基因启动子区存在较低程度的甲基化,这提示SYK基因启动子区出现的甲基化或许与鼻咽癌的发生相关。
Abstract:
Objective To detect methylation of spleen tyrosine kinase(SYK)gene promoter region in nasopharyngeal carcinoma,and to explore relationship between carcinogenesis of nasopharyngeal carcinoma and methylation of SYK gene promoter region.Methods A total of 52 patients with nasopharyngeal carcinoma and 26 patients with chronic rhinitis from Baoan People's Hospital of Shenzhen Hospital of Peking University were enrolled in this study between February 2012 and August 2014.All cases were diagnosed bypathological examination.Methylation specific-polymerase chain reaction assaywas performed to detect methylation status of SYK gene promoter region,the rateof methylation was compared for patients with nasopharyngeal carcinoma and patients with chronic rhinitis.Results The methylation of promoter region of SYK gene was detected for 11 biopsy samples among 52 nasopharyngeal carcinoma patients,the methylation frequency was 21.2% in nasopharyngealcarcinoma biopsy samples,while methylation was not found in 26 chronic rhinitisbiopsy samples.Conclusion Low methylation of promoter region of SYK gene was found in nasopharyngeal carcinoma.It suggests that methylation of SYK gene promoter region may contribute to carcinogenesis of nasopharyngeal carcinoma.

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备注/Memo

备注/Memo:
基金项目:深圳市宝安区科技计划项目(No. 2014027)。 作者简介:李 璐(1975-),男,本科毕业,主管技师,主要从事实验诊断工作,E-mail:31656416@qq.com。
更新日期/Last Update: 2015-03-20