[1]邱 爽,孟瑞芳,蒋筱漪,等.血清免疫固定电泳、蛋白电泳、免疫球蛋白及轻链定量在诊断多发性骨髓瘤中的临床应用[J].现代检验医学杂志,2015,30(02):61-64.[doi:10.3969/j.issn.1671-7414.2015.02.019]
　QIU Shuang,MENG Rui-fang,JIANG Xiao-yi,et al.Clinical Application of Immunofixtion Electrophoresis, Serum Protein Electrophoresis and Immunoglobulins and Light Chain Quantitative Analysis in the Diagnosis of Multiple Myeloma[J].Journal of Modern Laboratory Medicine,2015,30(02):61-64.[doi:10.3969/j.issn.1671-7414.2015.02.019]
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血清免疫固定电泳、蛋白电泳、免疫球蛋白及轻链定量在诊断多发性骨髓瘤中的临床应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
61-64
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Clinical Application of Immunofixtion Electrophoresis, Serum Protein Electrophoresis and Immunoglobulins and Light Chain Quantitative Analysis in the Diagnosis of Multiple Myeloma
作者:
邱 爽; 孟瑞芳; 蒋筱漪; 张会英
北京积水潭医院检验科,北京 100035
Author(s):
QIU Shuang; MENG Rui-fang; JIANG Xiao-yi; ZHANG Hui-ying
Department of Clinical Laboratory,Beijing Jishuitan Hospital,Beijing 100035,China
关键词:
多发性骨髓瘤;  血清蛋白电泳;  免疫固定电泳;  免疫球蛋白及轻链定量;  κ/λ比值
分类号:
R733.3; R730.43
DOI:
10.3969/j.issn.1671-7414.2015.02.019
文献标志码:
A
摘要:
目的 探讨血清免疫固定电泳(IFE)、蛋白电泳(SPE)和免疫球蛋白定量在多发性骨髓瘤(MM)诊断中的作用。方法 选择2012年6月～2013年12月北京积水潭医院符合MM诊断标准的MM患者192例为研究对象,选择同期健康体检者30例为对照组,对MM患者进行免疫固定电泳、血清蛋白电泳和免疫球蛋白及轻链定量检测并对结果进行分析。结果 192例MM患者有120例血清蛋白电泳检出M带,检出率为62.5%; 免疫固定电泳174例检出M带,检出率为90.6%; 分型结果显示以IgG型最多(106例,55.2%),其中IgG-λ型66例(34.4%); IgA型次之(36例,18.8%),轻链型最低(24例,12.5%)。免疫球蛋白、轻链定量测定结果显示,在各型MM患者血清中出现相应免疫球蛋白及轻链的变化,与正常对照组比较差异具有统计学意义(P<0.05),检出率为67.7%(130/192)。无论是κ型还是λ型M蛋白,κ/λ比值均具有明显差异(P<0.05),检出率为85.4%(164/192)。结论 免疫固定电泳、免疫球蛋白及轻链定量、κ/λ比值测定等免疫学检测在MM诊断中有较好的检出率,能及时为临床提供有效的诊疗指标,减少误诊和漏诊的发生率。
Abstract:
Objective To study the effectiveness of the laboratory diagnosis of multiple myeloma(MM)patients with immunofixtion electrophoresis(IFE),protein electrophoresis(SPE)and immunoglobulins and light chain quantitative analysis.Methods Selected 192 MM patients and 30 healthy controls during June 2012 to December 2013 and analyzed the resultsof IFE,SPE and immunoglobulins,and light chain quantitative analysis in MM patients.Results M protein bands were seen in 120 cases(62.5%)by using SPE and M protein were positive in 174 cases(90.6%)among the192 MM patients by using IFE.IFE showed that IgG were maximum type of the M protein(106 of 192,55.2%).There were IgG-λ type 66 cases(34.4%),IgA type36 cases(18.8%)and free light chain type 24 cases(12.5%).Immunoglobulinsof different immunophenotypes had higher than the nomal group with serum immunoglobulin and light chain quantitative analysis(P<0.05).The detection ratewas 67.7%(130/192).Whatever κ-type M protein or λ-type M protein,the ratio of κ/λ was significantly abnormal(P<0.05).The detection rate was 85.4%(164/192).Conclusion The better detection rate ofimmunological techniques such as immunofixtion electrophoresis and immunoglobulinsquantitative analysis might provide valuable basis for the diagnosis and treatment of MM clinically and prevent misdiagnosis.

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备注/Memo

备注/Memo:
作者简介:邱 爽(1978-),女,硕士,主管技师,从事临床生化检验,Tel:010-58398872,E-mail:qiudashuang@126.com。 通讯作者:张会英,主任技师,主要从事临床生化检验及实验室管理。
更新日期/Last Update: 2015-03-20