[1]吴跃军,季明德,刘淑杰.阴道分泌物对女性尿液干化学白细胞酯酶测定的影响[J].现代检验医学杂志,2015,30(02):163-164.[doi:10.3969/j.issn.1671-7414.2015.02.052]
　WU Yue-jun,JI Ming-de,LIU Shu-jie.Influence of Vaginal Discharge on Dry Chemistry Determination of Leukocyte Esterase in Female Urine[J].Journal of Modern Laboratory Medicine,2015,30(02):163-164.[doi:10.3969/j.issn.1671-7414.2015.02.052]
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阴道分泌物对女性尿液干化学白细胞酯酶测定的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
163-164
栏目:
综合报道·经验交流
出版日期:
2015-03-20

文章信息/Info

Title:
Influence of Vaginal Discharge on Dry Chemistry Determination of Leukocyte Esterase in Female Urine
作者:
吴跃军1; 季明德1; 刘淑杰2
1.江苏省中医院检验科,南京 210029; 2.南京市妇幼保健院,南京 210004
Author(s):
WU Yue-jun1; JI Ming-de1; LIU Shu-jie2
1.Department of Clinical Laboratory, Jiangsu Provinical Hospital of Traditional Chinese Medicine,Nanjing 210029,China; 2.Nanjing Maternity and Child Care,Nangjing 210004,China
关键词:
目的 探讨阴道分泌物对女性尿液干化学白细胞酯酶测定的影响。方法 收集正常阴道分泌物Ⅰ; Ⅱ度标本21份和30份; 并测定其中的白细胞酯酶和鳞状上皮细胞;  再收集正常阴道分泌物标本; 从中分离出鳞状上皮细胞; 并配制成20个/μl和60个/μl两个浓度的鳞状上皮细胞混悬液。将其加入正常女性尿液后进行干化学白细胞酯酶测定;  对于干化学白细胞酯酶测定强阳性而尿沉渣白细胞计数正常的患者做外阴清洗后再取中段尿做尿常规检验。结果 正常阴道分泌物中有66.7%的白细胞酯酶结果呈阳性; 且均有～的鳞状上皮细胞(镜检);  正常尿
分类号:
R446.19
DOI:
10.3969/j.issn.1671-7414.2015.02.052
文献标志码:
A
摘要:
目的 探讨阴道分泌物对女性尿液干化学白细胞酯酶测定的影响。方法 收集正常阴道分泌物Ⅰ,Ⅱ度标本21份和30份,并测定其中的白细胞酯酶和鳞状上皮细胞; 再收集正常阴道分泌物标本,从中分离出鳞状上皮细胞,并配制成20个/μl和60个/μl两个浓度的鳞状上皮细胞混悬液。将其加入正常女性尿液后进行干化学白细胞酯酶测定; 对于干化学白细胞酯酶测定强阳性而尿沉渣白细胞计数正常的患者做外阴清洗后再取中段尿做尿常规检验。结果 正常阴道分泌物中有66.7%的白细胞酯酶结果呈阳性,且均有～的鳞状上皮细胞(镜检); 正常尿液组和加入低浓度(20个/μl)及高浓度(60个/μl)鳞状上皮细胞尿液组的尿液干化学白细胞酯酶测定差异无统计学意义(P>0.05); 再次中段尿的鳞状上皮细胞和白细胞干化学阳性率及强度较第一次尿明显降低,差异有统计学意义(P<0.05),而白细胞计数无明显区别,差异无统计学意义(P>0.05)。结论 阴道鳞状上皮细胞不会对尿液干化学白细胞酯酶的测定产生影响。但女性自身阴道分泌物中的白细胞酯酶对其尿液中的白细胞酯酶的测定有很大影响。
Abstract:
Objective The present study is to investigate the influence of vaginal discharge on dry chemistry determination of leukocyte esterasein female urine.Methods Collected 20 and 30 normal vaginal discharge samples.Which humoral routine test degree were Ⅰ and Ⅱ correspondingly,and then analyze leukocyte esterase and squamous epithelial cells in these normal vaginal discharge samples.Collected normal vaginal discharge samplesagain and isolate the vaginal squamous epithelial cells from those normal vaginal discharge samples.Made two concentration of squamous epithelial cell suspension liquid(20/μl and 60/μl)and added these liquid.To normal female urine and analyze the drying chemical leukocyte esterase in those urine.Cleaned the vulva of those patients with normal leukocyte counts in urine sediment determination,whose dry chemical determination of leukocyte esterase was strong positive,perform the routine urine test with her middle urine.Results The results of the determination of leukocyte esterase in normal vaginal discharge samples were 66.7% positive.And there were ～ squamous epithelialcells in all samples(microscopy).No significant difference was observed in theexamination of dry chemistry leukocyte esterase among the normal female urine group,low(20/μl)and high(60/μl)concentrations of squamous epithelial cellurine group(P>0.05).The counts of squamous epithelial cells and the rateof positive and intensity of dry chemical leukocyte esterase in the middle of second urine were significantly lower than before,and the differences are statistically significant(P<0.05).But there was no significant difference for leukocytes counts.Conclusion Urine squamous epithelial cells had no influence on the detection of leukocyte esterase by dry chemistry.However,the leukocyte esterase in the vaginal discharge greatly influenced the examination of the leukocyte esterase in urine.

参考文献/References:

[1] 胡继达.白细胞酯酶活性测定在阴道炎诊断中的临床价值[J].现代医药卫生,2012,28(14):2185-2186. Hu JD.Clinical value of leukocyte esterase test in colpitis[J].JMod Med Health,2012,28(14):2185-2186.
[2] 苏希跃.检验分析前质量控制[J].国际检验医学杂志,2007,28(12):1139-1140. Su XY.Quality control before analysis[J].International Journalof Laboratory Medicine,2007,28(12):1139-1140.

备注/Memo

备注/Memo:
作者简介:吴跃军(1975-),男,硕士,副主任技师,研究方向:生物化学,Tel,13813964310,E-mail:yangniu1845@sina.com。
更新日期/Last Update: 2015-03-20