[1]李 颖a,易 默b,何小勤b.Reprimo和hMLH1基因甲基化在胃癌早期诊断价值的研究[J].现代检验医学杂志,2015,30(03):53-55.[doi:10.3969/j.issn.1671-7414.2015.03.015]
 LI Yinga,YI Mob,HE Xiao-qinb(a..Research on Reprimo,hMLH1 Gene Methylation in Early Diagnosis Value of Gastric Cancer[J].Journal of Modern Laboratory Medicine,2015,30(03):53-55.[doi:10.3969/j.issn.1671-7414.2015.03.015]
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Reprimo和hMLH1基因甲基化在胃癌早期诊断价值的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年03期
页码:
53-55
栏目:
论著
出版日期:
2015-06-10

文章信息/Info

Title:
Research on Reprimo,hMLH1 Gene Methylation in Early Diagnosis Value of Gastric Cancer
文章编号:
1671-7414(2015)03-053-04
作者:
李 颖a易 默b何小勤b
陕西省人民医院 a.腔镜中心; b.消化内二科,西安 710068
Author(s):
LI YingaYI MobHE Xiao-qinb(a.
a.Department of Endoscopy Center;
b.Department of Gastroenterology,Shaanxi Provincial People's Hospital,Xi'an 710068,China
关键词:
Reprimo基因 hMLH1 基因 胃癌早期诊断
分类号:
R735.2; R730.43
DOI:
10.3969/j.issn.1671-7414.2015.03.015
文献标志码:
A
摘要:
目的 探讨Reprimo和hMLH1基因启动子区甲基化检测在胃癌早期诊断中的价值。方法 通过陕西省人民医院2013年9月~2014年4月收治的慢性萎缩性胃炎伴肠上皮化生患者50例、异型增生患者50例、胃癌患者50例,取内镜下胃活检组织,并取正常胃黏膜活检组织30例作为对照组,分析Reprimo基因和hMLH1基因启动子区甲基化表达情况,比较各组患者间的差异。结果 患者组的胃黏膜组织中Reprimo基因和hMLH1基因启动子区甲基化阳性率显著高于正常组,差异有统计学意义(P<0.05)。Reprimo基因启动子区甲基化阳性率:慢性萎缩性胃炎伴肠上皮化生组28%(χ2=10.18,P<0.05); 异型增生组56%(χ2=25.84,P<0.05); 胃癌组62%(χ2=30.36,P<0.05); hMLH1基因启动子区甲基化阳性率:慢性萎缩性胃炎伴肠上皮化生组20%(χ2=4.39,P<0.05); 异型增生组44%(χ2=15.13,P<0.05); 胃癌组48%(χ2=17.41,P<0.05),检测的特异度高。结论 Reprimo和hMLH1基因甲基化检测在胃癌早期诊断中的价值很高,特异度高,是一种有效的诊断方式,在患者的临床诊断方面拥有广阔的治疗前景。
Abstract:
Objective To investigate the Reprimo and hMLH1gene promoter methylation detection value in the diagnosis of early gastric cancer.Methods Chose patints in Shaanxi Provincial People'sHospital from September 2013 to April 2014,50 cases of patients with chronic atrophicgastritis with intestinal metaplasia,50 cases of patients with gastricmucosal atypical hyperplasia,50 patients with gastric cancer,endoscopic gastricbiopsy samples,and 30 cases of normal gastric mucosa biopsy tissues as controlgroup.Analysis abnormal expression in Reprimo gene and hMLH1 genes promoter methylation,compared the differences.between groups of patients.Results The patients with gastric mucosatissues Reprimo and hMLH1 genes promoter methylation positive rate was significantly higher than that of normal group,the difference wasstatistically significant(P<0.05).Reprimo gene promoter methylation were:patients with chronic atrophic gastritis with intestinal metaplasia 28%(χ2=10.18,P<0.05).Patients with gastric mucosalatypicalhyperplasia 56%(χ2=25.84,P<0.05)and patients with gastric cancer 62%(χ2=30.36,P<0.05).hMLH1 gene promoter methylation were:patients with chronic atrophic gastritis with intestinal metaplasia 20%(χ2=4.39,P<0.05),patients with gastric mucosal atypical hyperplasia 44%(χ2=15.13,P<0.05)and patients with gastric cancer 48%(χ2=17.41,P<0.05),high specificity of detection.Conclusion Reprimo and hMLH1 gene's detect value in the diagnosis of early gastric cancer is very high,high specificity,it is an effective way of diagnosis,treatment in clinical diagnosis of patients with broad prospects.

参考文献/References:

[1] Dirnu R,Secureanu FA,Neamtu C,et al.Chronic ga-stritis withintestinal metaplasia:clinico-statistical, histological and immunohistochemical study[J].Rom J Morphol Embryol,2012,53(2):293-297.
[2] Tsukamoto T, Mizoshita T, Tatematsu M.Gastric-and-intestinal mixed-type intestinal metaplasia:aberrant expression of transcription factors and stem cell intestinalization[J].Gastric Cancer,2006,9(3):156-166.
[3] Yamamoto E,Suzuki H,Takamaru H,et al.Role of DNA methylation in the development of diffuse-type gastrie cancer[J].Digestion,2011,83(4):241-249.
[4] Fock KM,Talley N,Moayyedi P,et al.胃癌预防亚太地区共识与指南[J].胃肠病学,2008,13(4):231-240.
[5] Nomura S,Kaminishi M.Surgical treatment of early gastric cancer[J].Dig Surg,2007,24(2):96-100.
[6] Mir MR,Shabir N,Wani KA,et al.Association between p16,hMLH1 and E-cadherin promoter hypermethylation and intake of local hot salted tea and sun-dried foods in Kashmiris with gastric tumors[J].Asian PacJ Cancer Prev,2012,13(1):181-186.
[7] Alves MK,Ferrasi AC,Lima VP,et al.Inactivation of COX-2,HMLH1 and CDKN2A gene by promoter methylation in gastric cancer:relationship with histological subtype,tumor location and Helicobacter pylori genotype[J].Pathobiology,2011,78(5):266-276.
[8] Xiao XQ,Gong WD,Wang SZ,et al.Polymorphisms of mismatch repairgene hMLH1 and hMSH2 and risk of gastric cancer in a Chinese population[J].Oncol Lett,2012,3(3):591-598.
[9] Ling ZQ,Tanaka A,Li P,et al.Microsatellite instability with promotermethylation and silencing of hMLH1 can regionally occur during progression of gastric carcinoma[J].Cancer Lett,2010,297(2):244-251.
[10] Bernal C,Aguayo F,Villarroel C,et al.Reprimo as a potentialbiomarker for early detection in gastric cancer[J].Clin Cancer Res,2008,14(19):6264-6269.

备注/Memo

备注/Memo:
基金项目:陕西省科技厅社发攻关项目(2013K12-03-18)。
作者简介:李 颖(1971-),女,消化内科副主任医师,长期从事消化内镜的诊治工作,研究方向:消化道疾病的内镜诊治,Tel:18 291937986,E-mail:879359091@qq.com。
更新日期/Last Update: 2015-06-10