[1]钟 堃,王 薇,何法霖,等.半胱氨酸蛋白酶抑制剂C和β2微球蛋白参考区间的调查与分析[J].现代检验医学杂志,2015,30(04):32-34.[doi:10.3969/j.issn.1671-7414.2015.04.008]
　ZHONG Kun,WANG Wei,HE Fa-lin,et al.Investigation and Analysis of Reference Intervals of Cystatin C and β2-Microglobulin[J].Journal of Modern Laboratory Medicine,2015,30(04):32-34.[doi:10.3969/j.issn.1671-7414.2015.04.008]
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半胱氨酸蛋白酶抑制剂C和β2微球蛋白参考区间的调查与分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
32-34
栏目:
论著
出版日期:
2015-08-10

文章信息/Info

Title:
Investigation and Analysis of Reference Intervals of Cystatin C and β2-Microglobulin
文章编号:
1671-7414(2015)04-032-04
作者:
钟 堃; 王 薇; 何法霖; 张 妍; 赵 彦; 李国华; 王治国
北京医院卫生部临床检验中心,北京 100730
Author(s):
ZHONG Kun; WANG Wei; HE Fa-lin; ZHANG Yan; ZHAO Yan; LI Guo-hua; WANG Zhi-guo
Beijing Hospital,National Center for Clinical Laboratories,Beijing 100730,China
关键词:
半胱氨酸蛋白酶抑制剂C;  β2微球蛋白;  参考区间
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2015.04.008
文献标志码:
A
摘要:
目的 了解医疗机构中半胱氨酸蛋白酶抑制剂C和β2微球蛋白这两个肾功能指标参考区间的应用现状。方法 通过互联网向2014年参加卫生部临床检验中心特殊蛋白室间质量评价计划的单位发送半胱氨酸蛋白酶抑制剂C和β2微球蛋白的参考区间调查表,收到反馈结果后使用Microsoft Excel 2007和SPSS 13.0进行统计分析。内容包括:参考区间来源、统计学分析、正态性检验、不同检测系统与总体结果的比较,以及参考区间应用前的验证情况。结果 分别有106和127家实验室上报了半胱氨酸蛋白酶抑制剂C和β2微球蛋白的结果,分别覆盖了24和29个省、自治区和直辖市。只有5家和2家实验室进行了参考区间的性别分组,有3家实验室进行了参考区间的年龄分组。约75%的实验室采用试剂厂家说明书所给出的参考区间。所有组的组中位数和众数基本一致。所有项目和分组第2.5和第97.5百分位数差异较大。半数以上的实验室都未进行参考区间的验证。参考区间下限和上限的中位数分别为(血清中):半胱氨酸蛋白酶抑制剂C(mg/L)0.55和1.09; β2微球蛋白(μg/ml)1.00和2.80。结论 目前半胱氨酸蛋白酶抑制剂C和β2微球蛋白检验项目所应用的参考区间有一定的差异,所用仪器试剂和检测系统多样,建议建立适用于我国,并且较为规范和统一的参考区间。该次调查所得两个项目参考区间为:半胱氨酸蛋白酶抑制剂C:0.55～1.09 mg/L; β2微球蛋白:1.00～2.80 μg/ml。
Abstract:
Objective To know the current application status of reference interval of cystatin C and β2-microglobulin in medical institutions of China.Methods Every laboratory which attended 2014 specific protein external quality assessment program of national center of clinical laboratories has been received an internet questionnaire to survey reference intervals of cystatin C and β2-microglobulin.After eliminating outliersthe trimmed data was statistical analyzed by Microsoft Excel 2007 and SPSS 13.0.The resources of RIs,traditional statistical analysis,normalized analysis,comparison between overall median and lower and upper limits of different measurement system,and verification status of RIs were included.Results106 and 127 medical laboratories submitted the questionnaire and thenumber of province with submission of RIs for the two renal function measurementitems were 24 and 29,respectively.Less than 5 laboratories grouped their RIsaccording to age and/or gender.Approximately 75% of laboratories had been usingthe RIs referring to reagent manufacturers'instructions.All medians of upperor lower limits of analytes were as same as the Mode values of the same group.The 2.5th percentiles had tremendous differences compared with 97.5th percentiles.More than half of laboratories did not verify their RIs beforeusing them.The overall medians of lower and upper limits of RIs were(in serum):Cystatin C(mg/L)0.55 and 1.09; β2-microglobulin(μg/ml)1.00 and 2.80.Conclusion The RIs of cystatin C and β2-microglobulin used in medical institution had some differences between each other,and there were too manykinds of reagents and instruments which made different measurement systems.Theconsistent and standardized reference intervals should be established in a shorttime.The RI for cystatin C was 0.55～1.09 mg/L,for β2-microglobulin was 1.00～2.80 μg/ml considered by this study.

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备注/Memo

备注/Memo:
基金项目:北京市自然科学基金资助项目(7143182)。
作者简介:钟 堃(1984-),男,硕士研究生,助理研究员,主要从事临床实验室管理研究工作。
通讯作者:王治国,男,硕士研究生导师,Tel:010-58115054,E-mail:zgwang@nccl.org.cn。
更新日期/Last Update: 2015-08-10