[1]张 微,郭金金,李天君,等.北京地区献血人群人类巨细胞病毒及人类T淋巴细胞白血病病毒感染情况调查[J].现代检验医学杂志,2015,30(04):35-38.[doi:10.3969/j.issn.1671-7414.2015.04.009]
　ZHANG Wei,GUO Jin-jin,LI Tian-jun,et al.Investigation of Human Cytomegalovirus and Human T Lymphotropic Virus Infection of Voluntary Blood Donors in Beijing Area[J].Journal of Modern Laboratory Medicine,2015,30(04):35-38.[doi:10.3969/j.issn.1671-7414.2015.04.009]
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北京地区献血人群人类巨细胞病毒及人类T淋巴细胞白血病病毒感染情况调查()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
35-38
栏目:
论著
出版日期:
2015-08-10

文章信息/Info

Title:
Investigation of Human Cytomegalovirus and Human T Lymphotropic Virus Infection of Voluntary Blood Donors in Beijing Area
文章编号:
1671-7414(2015)04-035-04
作者:
张 微; 郭金金; 李天君; 张文学; 郭崇健; 宋 艳; 车 进; 张燕华; 李美霖
北京市通州区中心血站,北京 101100
Author(s):
ZHANG Wei; GUO Jin-jin; LI Tian-jun; ZHANG Wen-xue; GUO Chong-jian; SONG Yan;  CHE Jin; ZHANG Yan-hua; LI Mei-lin
Tongzhou Central Blood Station of Beijing,Beijing 101100,China
关键词:
北京地区;  献血人群;  人类巨细胞病毒;  人类T淋巴细胞白血病病毒
分类号:
R457.12; R373
DOI:
10.3969/j.issn.1671-7414.2015.04.009
文献标志码:
A
摘要:
目的 了解北京地区人类巨细胞病毒(HCMV)及人类T淋巴细胞白血病病毒(HTLV)在无偿献血人群中的感染情况。方法 北京地区2 010例无偿献血者血液标本采用ELISA方法筛查HCMV-IgG,HCMV-IgM,HTLV-Ⅰ/Ⅱ抗体。初检阳性标本进行双孔复检,两次检测结果均为阳性标本判定为ELISA结果阳性,HTLV阳性样品经巢式PCR检测核酸进行确认。结果 北京地区2 010例无偿献血者中HCMV-IgM和HCMV-IgG阳性率分别为2.19%和92.59%,ELISA法初筛抗-HTLV阳性1例,巢式PCR确认结果阴性。结果 通过χ2检验统计学分析显示,无偿献血者HCMV-IgG阳性率男性低于女性(χ2=5.88,P＜0.05),18～25岁年龄段的献血者HCMV-IgG和HCMV-IgM阳性率低于其他年龄段(χ2=16.51,21.52; 均P＜0.05),大学生HCMV-IgG阳性率低于其他职业献血者(χ2=14.20,P＜0.05),而献血者教育程度与HCMV-IgG和HCMV-IgM阳性率无关(P＞0.05)。结论在该次调查中,北京地区2 010例无偿献血者中未发现感染HTLV的病例,而HCMV感染率与性别、年龄、职业相关,与教育程度无关。
Abstract:
Objective To analysis the human cytomegalovirus(HCMV)and human T lymphotropic virus(HTLV)infection status in Beijing among voluntary blood donors.Methods Randomly selected 2 010 blood samples from five districts and counties of Beijing City to screen HCMV-IgG,HCMV-IgM and HTLV-Ⅰ/Ⅱ antibody by ELISA method.The positive samples werereexamined two times,two test results of samples were positive that were determined positive by ELISA.HTLV positive samples was confirmed by nested PCR.Results The HCMV-IgM and HCMV-IgG positive rates of Beijingblood donors were 2.19% and 92.59%,screened 1 case of anti-HTLV positive byELISA method,then confirmed to be negative result by nested PCR.The statisticsshowed that the HCMV-IgG positive rate female blood donors was higher than male(P＜0.05).The positive rates of HCMV-IgM and HCMV-IgG among18～25 yearsold donors was lowest(P＜0.05).Positive rate of HCMV-IgG in college students was lower than other occupation blood donars(P＜0.05)and education degree was independent of HCMV-IgG,HCMV-IgM positive rates(P＞0.05).Conclusion In this investigation,2 010 cases of voluntary blood donors from five districts of Beijing were not found in cases of HTLV infection,HCMV infection was prevalent.

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备注/Memo

备注/Memo:
基金项目:北京市科技工作基础专项(项目编号:2013004)。
作者简介:张 微(1981-),女,硕士研究生,主管检验师,从事血液学检验,E-mail:zhangwei810131@163.com。
通信作者:李天君,男,主任技师,E-mail:xzltj@hotmail.com。
更新日期/Last Update: 2015-08-10