[1]刘运端,林世恒.脂联素及其基因多态性与广西瑶族冠心病的关系[J].现代检验医学杂志,2015,30(04):56-59.[doi:10.3969/j.issn.1671-7414.2015.04.015]
　LIU Yun-duan,LIN Shi-heng.Study on Relations between Adiponectin and Its Gene Polymorphism and Yao Coronary Heart Disease of Guangxi District[J].Journal of Modern Laboratory Medicine,2015,30(04):56-59.[doi:10.3969/j.issn.1671-7414.2015.04.015]
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脂联素及其基因多态性与广西瑶族冠心病的关系()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
56-59
栏目:
论著
出版日期:
2015-08-10

文章信息/Info

Title:
Study on Relations between Adiponectin and Its Gene Polymorphism and Yao Coronary Heart Disease of Guangxi District
文章编号:
1671-7414(2015)04-056-04
作者:
刘运端; 林世恒
广西玉林市红十字会医院检验科,广西玉林 537000
Author(s):
LIU Yun-duan; LIN Shi-heng
Department of Clinical Laboratory, Yulin Red Cross Hospital in Guangxi District,Guangxi Yulin 537000,China
关键词:
脂联素;  基因多态性;  瑶族;  冠心病
分类号:
R541.4; Q786
DOI:
10.3969/j.issn.1671-7414.2015.04.015
文献标志码:
A
摘要:
目的 探讨广西地区瑶族冠心病患者血清中脂联素(APN)水平及其基因位点+276 G/T和-11377 C/G的多态性。方法 瑶族、汉族冠心病患者、瑶族和汉族正常人各100例纳入研究。采用酶联免疫吸附法(ELISA)检测研究对象血清中APN水平,聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测APN基因位点+276G/T和-11377C/G的多态性。结果 ①两组冠心病组血清中APN水平明显低于两组正常组,差异有统计学意义(t=10.311,8.642; 均P=0.000)。瑶族冠心病组和汉族冠心病组血清中APN的差异无统计学意义(t=1.792,P=0.076); ②在H-W平衡检验中,四组中APN基因位点+276G/T和-11377C/G均具有群体代表性(均P＞0.05); ③四组APN+276G/T位点的主要型别是野生型。4组间APN+276G/T位点的等位基因频率均无显著差异(均P＞0.05); ④冠心病组ANP-11377C/G基因型和等位基因频率与同民族正常组的差异有统计学意义(χ2=8.908,P=0.012; χ2=17.275,P=0.000); 瑶族冠心病组APN-11377C/G基因杂合程度略低于汉族冠心病组,但差异无统计学意义(χ2=0.363,P=0.834)。结论 同汉族冠心病一样, 血清低APN水平和APN-11377C/G位点是瑶族冠心病患者的危险因素。
Abstract:
Objective To investigate the difference of serum levels of adiponectin(APN)and its gene polymorphism +276 G/T和-11377 C/G inYao patients with CHD and Han CHD patients and Yao normal people in Guanxi district.Methods Yao CHD patients,Han CHD patients,Yao normal people and Han normal people,each of 100 cases were included in the study.The levels of serum adiponectin of all research object were detected by ELISAand APN+276 G/T and APN-11377 C/G genotyping were conducted by using PCR-RFLP.Results The levels of adiponectin in two groups of CHDpatients were significantly lower than same race normal groups,the difference were statically significant(t=10.311,8.642,all P=0.000).The adiponectin levels of two CHD groups was not statically significant difference(t=1.792,P=0.076); ②The APN+276 G/T and APN-11377 C/G of four groups conformthe Hardy-Weinberg population genetic equilibrium law(all P＞0.05); ③Themain type of all groups APN+276 G/T was the wild type.The genotype frequenciesof APN+276 G/T were no significant differences in between 4 groups(all P＞0.05); ④The CG and GG genotype frequency of APN-11377 C/G in two CHD groups were higher than same race normal group,the difference of Yao CHD group and Yao normal group was statically significant(χ2=8.908,P=0.012; χ2=17.275,P=0.000),and the difference of Yao CHD group and Han CHD group were not statically significant(χ2=0.363,P=0.834).Conclusion As with Han CHD patients,serum low APN level and APN-11377 C/G loci maybe risk factors of Yao CHD patients.

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备注/Memo

备注/Memo:
作者简介:刘运端(1981-),男,大学本科,主管检验技师,医学检验工作,研究方向:医学免疫学与生化检验,Tel:13977544498,E-mail:490835268@qq.com。
更新日期/Last Update: 2015-08-10