[1]徐鸣皋,丁进亚,徐 娟.两种全自动微生物鉴定系统在布鲁菌鉴定中的应用比较[J].现代检验医学杂志,2015,30(04):105-107.[doi:10.3969/j.issn.1671-7414.2015.04.030]
　XU Ming-gao,DING Jin-ya,XU Juan.Comparison of Two Kinds of Automated Microbial Identification System Used in the Identification of Brucella[J].Journal of Modern Laboratory Medicine,2015,30(04):105-107.[doi:10.3969/j.issn.1671-7414.2015.04.030]
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两种全自动微生物鉴定系统在布鲁菌鉴定中的应用比较()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
105-107
栏目:
研究简报·实验技术
出版日期:
2015-08-10

文章信息/Info

Title:
Comparison of Two Kinds of Automated Microbial Identification System Used in the Identification of Brucella
文章编号:
1671-7414(2015)04-105-03
作者:
徐鸣皋,丁进亚,徐 娟
广州军区武汉总医院检验科,武汉 430070
Author(s):
XU Ming-gao,DING Jin-ya,XU Juan
Department of Laboratory Medicine, Wuhan General Hospital of Guangzhou Command,Wuhan 430070,China
关键词:
全自动微生物鉴定系统;  布鲁菌;  鉴定;  16S rRNA基因序列
分类号:
R446.5
DOI:
10.3969/j.issn.1671-7414.2015.04.030
文献标志码:
A
摘要:
目的 比较Vitek2 Compact和Walkaway 40全自动微生物鉴定系统在布鲁菌鉴定中的应用。方法 临床分离菌株,采用Vitek2 Compact和Walkaway 40全自动微生物鉴定系统进行鉴定,并将鉴定结果和16S rRNA基因序列检测的结果进行比较。结果 临床分离菌株经Vitek2 Compact GN鉴定卡鉴定为马耳他布鲁菌,Walkaway 40 NC31鉴定卡鉴定为动物溃疡威克斯菌、莫拉菌属等。16S rRNA基因序列分析表明,该临床分离菌株的16S rRNA基因序列与布鲁菌匹配,排除动物溃疡威克斯菌、莫拉菌属等的可能,确定该菌株为马耳他布鲁菌。结论 Vitek2 Compact可以准确的鉴定出布鲁菌,经Vitek2 Compact鉴定为布鲁菌后,可采用分子生物学方法进行佐证,大大地提高了布鲁菌的检出率,降低误诊的可能性。Walkaway 40无法准确的鉴定出布鲁菌,误鉴定会导致错误的治疗并使工作人员处于实验室获得性感染的危险之中。建议使用Walkaway 40的实验室在鉴定出动物溃疡威克斯菌或莫拉菌属等细菌时应谨慎报告,并使用Vitek2 Compact或分子生物学方法进行复查。
Abstract:
Objective To compare Vitek2 Compact and Walkaway 40 in the identification of Brucella.Methods UsedVitek2 Compact and Walkaway 40 automated microbial identification system to identify clinical isolates and compared with the detection of 16S rRNA gene sequences analysis.Results The clinical isolates was identifiedas Bergeyella zoohelcum or Moraxella by Walkaway 40 and as Brucellamelitensis by Vitek2 Compact.16S RNA sequence analysis of the isolate,the sequence was identical to the sequences of 16S rRNA of Brucella,which excludedthe possibility of B.zoohelam and Moraxella.Determined that the isolate was B.melitensis.Conclusion Vitek 2 Compact can accurately identified Brucella.Use molecular methods to corroborate when theisolates was identified as Brucella by Vitek 2 Compact,this method can greatly improve the detection rate of brucellosis and reduce the possibility of misdiagnosis.Walkaway 40 cannot accurately identified Brucella,Misidentification of Brucella can result in wrong treatment of the patient and let the staff in the risk of laboratory-acquired infection.Recommend laboratory should becautious reporting in the identified B.zoohelam or Moraxella by Walkaway 40 and use Vitek2 Compact or molecular methods for review.

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备注/Memo

备注/Memo:
作者简介:徐鸣皋(1982-),男,本科,技师,研究方向:临床微生物检验,Tel:13163273392,E-mail:117242829@qq.com。
更新日期/Last Update: 2015-08-10