[1]马静瑶,郭 滔.游离血红蛋白室内质控物的制备及应用[J].现代检验医学杂志,2015,30(04):117-119.[doi:10.3969/j.issn.1671-7414.2015.04.034]
　MA Jing-yao,GUO Tao.Preparation and Application of Internal Quality Control Substance of Free Hemoglobin[J].Journal of Modern Laboratory Medicine,2015,30(04):117-119.[doi:10.3969/j.issn.1671-7414.2015.04.034]
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游离血红蛋白室内质控物的制备及应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
117-119
栏目:
质量控制·实验室管理
出版日期:
2015-08-10

文章信息/Info

Title:
Preparation and Application of Internal Quality Control Substance of Free Hemoglobin
文章编号:
1671-7414(2015)04-117-03
作者:
马静瑶; 郭 滔
淮北市中心血站,安徽淮北 235000
Author(s):
MA Jing-yao; GUO Tao
Huaibei Central Blood Station,Anhui Huaibei 235000,China
关键词:
游离血红蛋白;  质控物;  评价
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2015.04.034
文献标志码:
A
摘要:
目的 制备游离血红蛋白(FHb)室内质控物,并对其性能进行评价。方法 ①选取5份已知血红蛋白(Hb)浓度的正常人类全血,用蒸馏水分别以1:500,1:1 000,1:2 000,1:4 000的比例进行稀释,充分混匀后配制成20份FHb溶液,检测其FHb浓度,并与其理论浓度进行比较。②选择高值、低值溶液各1份,作为日常室内质控物,混匀分装-20℃保存,常规条件下连续检测20天,计算两组的(-overx),s和CV值,绘制Levey-Jennings质控图; 之后每周测定一次,将数据依次标记在质控图上,直至更换试剂批号; 计算高值、低值两种质控物整个检测周期内的总体(-overx),s和CV值。结果 ①该20份标本的FHb浓度理论值和实测值((-overx)±s)分别为125.44±93.04 mg/L和125.22±93.08 mg/L,差异无统计学意义(t=0.706,P＞0.05)。②高值、低值质控物连续20天检测结果((-overx)±s)分别为303.55±3.70 mg/L和69.29±1.88 mg/L,CV值分别为1.22%和2.68%; 高值、低值质控物整个检测周期内结果均未失控,总体((-overx)±s)分别为302.56±3.99 mg/L和69.04±1.88 mg/L,CV值分别为1.32%和2.71%。结论 FHb室内质控物制备过程简单,质量可靠,稳定性好,适合在血站和临床实验室中应用和推广。
Abstract:
Objective To prepare an internal quality controlsubstance of free hemoglobin(FHb),and evaluate its performance.Methods ① 5 normal whole human blood samples with known hemoglobin(Hb)concentration was selected and diluted with distilled water in the ratio of 1:500,1:1 000,1:2 000,1:4 000 respectively.After 20 FHb solutions was fully mixed,the concentration of FHb solutions was tested and compared with its theoretical data.②One of the highest value and the lowest value solutions were chosen as qualitye control substance,packed and stored in -20℃ fridge.Each group was analyzed 20 days continuously in the routine conditions,where average((-overx))of the groups,standards deviation(s)and coefficients of variation(CV)were calculated and Levey-Jennings control chart was conducted.Then,the test was repeated every week,and the data on the chart was updatedaccordingly until(CV)of the reagents were changed. The average((-overx))of thegroups,standards deviation(s)and coefficients of variation uality control material was calculated during the whole test period.Results ①The theoretical value((-overx)±s)of the concentration of free hemoglobin was 125.44±93.04 mg/L,and the actual value was 125.22±93.08 mg/L,there was no statistic significance(t value was 0.706,P＞0.05).② During 20 continuously daystest,the detected values((-overx)±s)of the highest quality control material was 303.55±3.70 mg/L; the values of the lowest quality control material was 69.29±1.88 mg/L.The coefficients were 1.22% and 2.68% respectively.Both the highest and the lowest quality control material were under control during the whole detecting period.The((-overx)±s)values of the highest was 302.56±3.99 mg/L,the CV valueof it was 1.32%; The((-overx)±s)values of the lowest was 69.04±1.88mg/L,the CV value of it was 2.71%.Conclusion Thepreparation method of internal quality control substance of free hemoglobin is convenient and stable,and was applicable in blood station's and clinical laboratory.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:马静瑶(1980-),女,硕士,主管检验师,主要从事血液学检验及质量控制工作,Tel:18956187629,E-mail:clear224@163.com。
更新日期/Last Update: 2015-08-10