[1]张瑞君a,段秉政,鞠春梅a,等.去白细胞悬浮红细胞储血袋两种放置方式的溶血性分析[J].现代检验医学杂志,2015,30(04):120-122.[doi:10.3969/j.issn.1671-7414.2015.04.035]
　ZHANG Rui-juna,DUAN Bing-zheng,JU Chun-meia,et al.Hemolytic Analysis of Two Kinds of Placement of Leukoreduced Suspension Red Blood Cells Storage Bag[J].Journal of Modern Laboratory Medicine,2015,30(04):120-122.[doi:10.3969/j.issn.1671-7414.2015.04.035]
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去白细胞悬浮红细胞储血袋两种放置方式的溶血性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
120-122
栏目:
研究简报·实验技术
出版日期:
2015-08-10

文章信息/Info

Title:
Hemolytic Analysis of Two Kinds of Placement of Leukoreduced Suspension Red Blood Cells Storage Bag
文章编号:
1671-7414(2015)04-120-03
作者:
张瑞君1a; 段秉政2; 鞠春梅1a; 宋艳红1b; 白 燕1b; 霍 蓉1b; 隋素琴1a
1.内蒙古医科大学第四附属医院 a.输血科; b.内科,内蒙古包头 014030;
2.内蒙古包头市中心医院输血科,内蒙古包头 014040
Author(s):
ZHANG Rui-jun1a; DUAN Bing-zheng2; JU Chun-mei1a; SONG Yan-hong1b; BAI Yan1b; HUO Rong1b;  SUI Su-qin1a
1a.Department of Blood Transfusion; 1b.Department of Internal Medicine, the Fourth Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia Baotou 014030,China;
2.Department of Blood Transfusion, Baotou Central Hospital,Inner Mongolia
关键词:
储血袋;  贮存时间;  放置方式;  游离血红蛋白;  溶血率
分类号:
R457.12
DOI:
10.3969/j.issn.1671-7414.2015.04.035
文献标志码:
A
摘要:
目的 探讨去白细胞悬浮红细胞储血袋两种不同放置方式对上清液游离血红蛋白(FHb)和红细胞溶血率的影响,保证临床输血的安全、有效。方法 选择20例无偿献血者每人400 ml的全血,24 ml内制成去白细胞悬浮红细胞制品,将其等量均匀分装成10袋,随机分成2组,每组5袋,一组以直立放置,一组以水平放置,在同一环境条件下储存,分别在储存的第7天,14,21,28,35天时随机从各组取出1袋血液,分别测定其FHb和红细胞溶血率,进行统计学分析。结果 FHb和红细胞溶血率在贮存的第21天时检测的结果,直立放置组分别为(217.310±48.477)mg/L和(0.250±0.056)%,水平放置组分别为(173.972±39.027)mg/L和(0.189±0.045)%,直立放置组的结果要高于水平放置组,统计学结果分别为t=3.114,P=0.003＜0.05和t=3.798,P=0.001＜0.05,差异有统计学意义。结论 在血液储存期内,储血袋水平方式放置可减少红细胞破坏,对血液的贮存更加有利。
Abstract:
Objective Discussion leukoreduction of red blood cells suspended in two different storage bag placement and hemolysis rate impact on the supernatant free hemoglobin(FHb),to ensure the clinical transfusionis safe and effective.Methods Selected 20 donors to sample 400 ml whole blood per person to make leukodepleted red blood cells,which were evenly divided into 10 bags.The 10 bags were randomly divided into two groups,one to the upright position,one group of horizontal.The two groups were stored under the same conditions.Respectively,in the 7,14,21,28,35 day,randomly removed one storage bag from each group,FHb and red blood cell hemolysis rate were measured and analyzed statistically.Results FHband hemolysis rate results stored in the first 21 days of testing,uprightgroupwere(217.310±48.477)mg/L and(0.250±0.056)%,respectively horizontal group(173.972±39.027)mg/L and(0.189±0.045)%,the results set upright than horizontal group,the results were statistically( t=3.114,P=0.003＜0.05 andt=3.798,P=0.001＜0.05),the difference was statistically significant.Conclusion In the blood storage period,storage bags can beplaced horizontally to reduce the destruction of red blood cells,blood storageis more favorable.

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备注/Memo

备注/Memo:
基金项目:内蒙古包头市卫生基金资助项目(wsjj2012067)。
作者简介:张瑞君(1971-),男,本科,副主任检验师,从事临床输血管理和免疫学研究,Tel:13704738921,E-mail:zhangruijun618@163.com。
通讯作者:鞠春梅(1973-),女,本科,主管检验师,从事临床医学检验,Tel:18647221649,E-mail:juchunmei1973@163.com。
更新日期/Last Update: 2015-08-10