[1]夏 勇,纪 玲,徐安平,等.Beckman Coulter实验室自动化系统生化免疫样品间携带污染评估与解除[J].现代检验医学杂志,2015,30(04):125-127.[doi:10.3969/j.issn.1671-7414.2015.04.037]
　XIA Yong,JI Ling,XU An-ping,et al.Evaluation and Disposal of the Carryover between the Chemistry and Immunoassay in Beckman Coulter Laboratory Automation System[J].Journal of Modern Laboratory Medicine,2015,30(04):125-127.[doi:10.3969/j.issn.1671-7414.2015.04.037]
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Beckman Coulter实验室自动化系统生化免疫样品间携带污染评估与解除()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
125-127
栏目:
研究简报·实验技术
出版日期:
2015-08-10

文章信息/Info

Title:
Evaluation and Disposal of the Carryover between the Chemistry and Immunoassay in Beckman Coulter Laboratory Automation System
文章编号:
1671-7414(2015)04-125-03
作者:
夏 勇; 纪 玲; 徐安平; 林 松; 薛 灏; 郑若洋
北京大学深圳医院检验科,广东深圳 518036
Author(s):
XIA Yong; JI Ling; XU An-ping; LING Song; XUE Hao; ZHENG Ruo-yang
Department of Clinical Laboratory,Shenzhen Hospital of Peking University,Guangdong Shenzhen,518036,China
关键词:
实验室自动化系统;  样品间;  携带污染
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2015.04.037
文献标志码:
A
摘要:
目的 评估Beckman Coulter实验室自动化系统生化免疫样品间携带污染情况,探讨不选择分杯模块时生化免疫共用一管血的可行性。方法将高浓度及不同样品量(2 000 μl,500 μl)低浓度的HCG样品按照一定顺序在Beckman AU5421全自动生化分析仪上检测生化项目,比较检测生化项目前后低浓度样品HCG含量的变化评估生化免疫样品间携带污染情况,并在增加Beckman AU5421全自动生化分析仪特殊清洗程序后重复试验。结果 在不同样品量(2 000 μl,500μl)条件下,Beckman AU5421全自动生化分析仪单模块及整机携带污染分别为5.44,15.47,23.51,45.96 ppm,不同样品量及单模块与整机间携带污染差异均有统计学意义(t=14.553,P＜0.001; t=5.527,P=0.005; t=3.985,P=0.016; t=20.457,P＜0.001); 增加Beckman AU5421特殊清洗程序后仅检出500 μl样品量时整机携带污染为0.22 ppm。结论 不选择分杯模块时,Beckman Coulter实验室自动化系统可使用特殊清洗程序解决生化免疫间携带污染。
Abstract:
Objective To evaluate the Carryover between thechemistry and immunoassay in Beckman Coulter Laboratory Automation System and decide to whether sharing samples for testing between chemistry and immunoassay systems or not.Methods According to a certain order,highconcentration samples and low concentration samples of HCG with different sample volume(500 μl,2 000 μl)were tested on Beckman AU5421 automatic biochemical analyzer.The HCG of low concentration samples were then tested to evaluate the carryover between the chemistry and immunoassay and explored the corrective procedure to deal with the carryover by increasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.Results Under different sample volume,the carryover in a single module and as a whole of the beckman AU5421 automatic biochemistry analyzer were 5.44,15.47,23.51 and45.96 ppm respectively(t=14.553,P＜0.001; t=5.527,P=0.005; t=3.985,P=0.016; t=20.457,P＜0.001).By increasing special cleaning process the carryover of 0.22 ppm was detected in 500 μl sample volume of the beckman AU5421 automatic biochemistry analyzer as a whole.Conclusion Thecarryover between the chemistry and immunoassay in Beckman Coulter Laboratory Automation System could been sovled by increasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.

参考文献/References:

[1] 国家食品药品监督管理局.YY/T0654-2008,全自动生化分析仪[S].北京:中国标准出版社,2008.
China Food and Drug Administration.YY/T0654-20 08,automatic chemistry analyzer[S].Beijing:China Standards Press,2008.
[2] 柯培锋,刘冬冬,徐建华,等.全自动生化分析仪携带污染的发现及其解决方案[J].广东医学,2014,35(11):1728-1731.
Ke PF,Liu DD,Xu JH,et al.The detection and treatment measures ofcarry-over of automatic chemistry analyzer[J].Guangdong Medical Journal,2014,35(11):1728-1731.
[3] 冼中任,王治伟,黄良正,等.全自动生化分析仪吸样致样本污染对乙型肝炎病毒检测的影响[J].九江医学,2009,24(4):9-11.
Xian ZR,Wang ZW,Huang LZ,et al.Contamination affecting HBV detection caused by Sample-to-sample carryover in automatic biochemistry analysis[J].Jiujiang Medical Journal,2009,24(4):9-11.
[4] Armbruster DA,Alexander DB.Sample to sample ca-rryover:a source of analytical laboratory error and its relevance to integrated clinical chemistry/immunoassay systems[J].Clin Chim Acta,2006,373(1/2):37-43.

备注/Memo

备注/Memo:
基金项目:北京大学深圳医院学科骨干科研基金启动项目(项目编号:201412)。
作者简介:夏 勇(1978-),男,硕士,副主任技师,主要从事临床生物化学方向的研究,E-mail:sunmoonrain78@163.com。
通信作者:纪 玲,主任技师,医学博士,E-mail:jilingdd@yahoo.com。
更新日期/Last Update: 2015-08-10