[1]杨茁a,刘凌华a,张利侠b,等.耐氟喹诺酮类鲍曼不动杆菌Parc的变异研究[J].现代检验医学杂志,2015,30(06):25-26.[doi:10.3969/j.issn.1671-7414.2015.06.007]
　YANG Zhuoa,LIU Ling-huaa,ZHANG Li-xiab,et al.Study on Parc Mutant in Fluoroquinolone-Resistant Clinical Isolates of Acinetobacter Baummanii[J].Journal of Modern Laboratory Medicine,2015,30(06):25-26.[doi:10.3969/j.issn.1671-7414.2015.06.007]
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耐氟喹诺酮类鲍曼不动杆菌Parc的变异研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年06期
页码:
25-26
栏目:
论著
出版日期:
2015-12-30

文章信息/Info

Title:
Study on Parc Mutant in Fluoroquinolone-Resistant Clinical Isolates of Acinetobacter Baummanii
作者:
杨茁a; 刘凌华a; 张利侠b; 孙莉a
陕西省人民医院，西安交通大学医学院第三附属医院a.呼吸二科；b.检验科，西安710068
Author(s):
YANG Zhuoa; LIU Ling-huaa; ZHANG Li-xiab; SUN Lia
a.Two Department of Breathing；b.Department of Clinical Laboratory，Shaanxi Provincial People’s Hospital，the Third of Affiliated Hospital of Medical School，Xi’an Jiaotong University，Xi’an 710068，China
关键词:
鲍曼不动杆菌; 氟喹诺酮; 药物耐受性; DNA拓扑异构酶(ATP水解)
分类号:
R378；Q754
DOI:
10.3969/j.issn.1671-7414.2015.06.007
文献标志码:
A
摘要:
目的研究鲍曼不动杆菌拓扑异构酶ⅣC亚基(ParC)的变异与其耐氟喹诺酮类(FQNL)的关系。方法收集临床分离耐喹诺酮鲍曼不动杆菌30株及敏感株10株，测定其对萘啶酸、环丙沙星、左旋氧氟沙星的最低抑菌浓度(MIC)，并对此30株菌ParC的基因(parC)进行PCR扩增和DNA序列的分析比较。结果在4株高度耐FQNL(MIC≥128 mg/L)和1株低度耐FQNL(MIC=64 mg/L)菌中存在ParC的变异，同FQNL耐药性相关的变异有丝氨酸Ser87(TCG)→Leu(TTG)。结论临床分离的鲍曼不动杆菌对喹诺酮类药物耐药的分子机制可表现为ParC基因87位氨基酸密码子突变，当鲍曼不动杆菌合并有ParC的变异时，其耐药性往往会增强。
Abstract:
ObjectiveTo study the relation between the alterations in the topoisomeraseⅣsubunit C (ParC) and fluoroquinolones(FQNL) resistance in Acinetobacter baummanii．MethodsUsed age the micro broth diluent to determine the MICs of three quinolones of 30 quinolone-resistant and 10 quinolone-susceptible isolates.The genes of ParC in the　30 strains were amplified by PCR and their DNA sequences were compared．ResultsAlteration in ParC existed in all 4 strains which were highly resistant to FQNL　(MIC≥128 mg/L) and one strain was moderately resistant to FQNL (MIC=64 mg/L)，and the alteration related to FQNL resistance was Ser87(TCG)→Leu(TTG)．ConclusionThe molecular mechanism of the quinolone resistant Acinetobacter baummanii isolated from clinics was mutations in the ParC gene at codon 87．When alteration occurs in ParC，the FQNL resistance of bacteria tends to intensify．

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：陕西省自然科学基础研究计划(2013JM4027)。 作者简介：李茁(1972-)，女，医学硕士，主任技师，主要从事细胞耐药机制的研究，E-mail：yangzhuo1972@163.com。
更新日期/Last Update: 1900-01-01