[1]于魁魁,谢凯,张潇丹,等.镇江地区儿童群体博卡病毒感染情况及基因组遗传特征[J].现代检验医学杂志,2015,30(06):67-71.[doi:10.3969/j.issn.1671-7414.2015.06.019]
 YU Kui-kui,XIE Kai,ZHANG Xiao-dan,et al.Human Bocavirus Infection and the Complete Genomes of the Representative Strains in Zhenjiang Area[J].Journal of Modern Laboratory Medicine,2015,30(06):67-71.[doi:10.3969/j.issn.1671-7414.2015.06.019]
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镇江地区儿童群体博卡病毒感染情况及基因组遗传特征()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年06期
页码:
67-71
栏目:
论著
出版日期:
2015-12-30

文章信息/Info

Title:
Human Bocavirus Infection and the Complete Genomes of the Representative Strains in Zhenjiang Area
作者:
于魁魁1谢凯1张潇丹2章震花3朱桂芳3
1.徐州市中心医院检验科,江苏徐州221003; 2.江苏大学基础医学与医学技术学院,江苏镇江212013; 3.江苏大学附属第四人民医院检验科,江苏镇江212001
Author(s):
YU Kui-kui1XIE Kai1ZHANG Xiao-dan2ZHANG Zhen-hua3ZHU Gui-fang3
1.Department of Clinical Laboratory,Xuzhou Central Hospital,Jiangsu Xuzhou 221003,China; 2.School of Medical Science and Laboratory Medicine,Jiangsu University, Jiangsu Zhenjiang 212013,China;3.Department of Clinical Laboratory, the Fourth Hospital of Jiangsu University,Jiangsu Zhenjiang 212001,China
关键词:
人博卡病毒分子流行病学系统进化分析基因型
分类号:
R373.1;Q786
DOI:
10.3969/j.issn.1671-7414.2015.06.019
文献标志码:
A
摘要:
目的了解镇江地区呼吸道和胃肠道疾病患儿博卡病毒(HBoV)流行状况,并通过对代表毒株的全基因组测序了解博卡病毒遗传特征。方法收集2010年1月~2011年12月期间镇江地区156份呼吸道疾病患儿鼻咽分泌物样品和310份急性胃肠炎患儿粪便样品,采用PCR方法检测HBoV基因;选取3株镇江地区HBoV代表毒株,测序获取全基因组序列,采用CLUSTAL和MEGA4.0等软件进行序列同源性及系统发育分析。结果HBoV在鼻咽分泌物样品中的检出率为2.56%,在粪便样品检出率为3.55%;HBoV基因序列均与GenBank中提交的HBoV-1型毒株高度同源(>95%),且与上海地区的7株HBoV-1和重庆地区的3株HBoV-1同源性最高;系统进化分析结果显示,3株HBoV-1代表株聚类在三个不同的类群中,其中ZJ92与来自中国香港的HBoV毒株HK24及上海的HBoV毒株SH8遗传关系最近,ZJ42与来自泰国的毒株CU25遗传关系最近,而ZJ68与来自上海的R3080522001和SH1亲缘关系最近。结论HBoV-1为镇江地区HBoV主要流行基因型;系统进化分析显示镇江地区的HBoV-1毒株与重庆和上海的毒株有共同的分子起源。
Abstract:
ObjectiveTo investigate the prevalence of human bocaviruses(HBoV) in children population with gastrointestinal and respiratory diseases in Zhenjiang area,and to determine the genetic characters by the complete genome sequence of the representative strains.MethodsA total of 156 nasopharyngeal aspirate fluid samplesfrom children with respiratory diseases and 310 fecal samples from children with acute gastroenteritis were collected from Hospitals in Zhenjiang from Jan.2010 to Dec.2011.HBoV were detected by PCR and the complete sequences of 3 representative strains were determined by sequencing.Phylogenetic analysis were performed using Clustal W and Mega 4.0 softwares.ResultsHBoV were detected in 2.56% of the nasopharyngeal secretions samples and 3.55% of the stool samples.The sequence of HBoV showed high similarity to HBoV-1,which shared >95% sequence identities with 7 strains from Shanghai and 3 strains from Chongqing.Phylogenetic analysis revealed that the three representative strains were clustered in three different genetic groups,in which the ZJ92 strain showed close relationship with HK24 from Hong Kong and SH8 from Shanghai,the ZJ42 showed close relationship with CU25 from Thailand,and the ZJ68 showed close relationship with R3080522001 and SH1 from Shanghai.ConclusionHBoV-1 was the most prevalent genotype in Zhenjiang area.Phylogenetic analysis showed the HBoV-1 strains in Zhenjiang had the common infection resources with those from Shanghai and Chongqing.

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备注/Memo

备注/Memo:
作者简介:于魁魁,女,本科,学士学位,初级技师,研究方向:临床病毒学诊断技术,Tel:18112007752。 通讯作者:谢凯(1983-),男,本科,学士学位,初级技师,研究方向:临床病毒学诊断技术,E-mail:xhxsgm@163.com。
更新日期/Last Update: 1900-01-01