[1]杨晓玲,何大保,李文郎.急性白血病患者骨髓细胞LIMD1，VEGF-C，CTGF和Survivin的mRNA异常表达及相关性研究[J].现代检验医学杂志,2015,30(06):72-74.[doi:10.3969/j.issn.1671-7414.2015.06.020]
　YANG Xiao-ling,HE Da-bao,LI Wen-lang.Relationship Study on Abnormal Expression of the mRNA of LIMD1， VEGF-C，CTGF and Survivin in Patients with Acute Leukemia[J].Journal of Modern Laboratory Medicine,2015,30(06):72-74.[doi:10.3969/j.issn.1671-7414.2015.06.020]
点击复制

急性白血病患者骨髓细胞LIMD1，VEGF-C，CTGF和Survivin的mRNA异常表达及相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年06期
页码:
72-74
栏目:
论著
出版日期:
2015-12-30

文章信息/Info

Title:
Relationship Study on Abnormal Expression of the mRNA of LIMD1， VEGF-C，CTGF and Survivin in Patients with Acute Leukemia
作者:
杨晓玲1; 何大保2; 李文郎2
1.深圳市宝安区松岗人民医院检验科，广东深圳518105； 2.深圳市龙华新区中心医院检验科，广东深圳518110
Author(s):
YANG Xiao-ling1; HE Da-bao2; LI Wen-lang2
1.Department of Clinical Laboratory，the Songgang People’s Hospital of Baoan District， Guangdong Shenzhen 518105，China；2.Department of Clinical Laboratory， the Central Hospital of Longhua New District，Guangdong Shenzhen 518110，China
关键词:
急性白血病; LIM结构域蛋白1; 骨髓细胞; 原癌基因
分类号:
R557；Q786
DOI:
10.3969/j.issn.1671-7414.2015.06.020
文献标志码:
A
摘要:
目的研究LIM结构域蛋白1抗体在急性白血病患者的异常表达及相关性。方法选择首次确诊且未接受过治疗的急性白血病患者纳入初治组、接受化疗并达到完全缓解的白血病患者纳入缓解组、骨髓象正常的非恶性血液疾病患者纳入对照组，分离培养骨髓细胞，检测细胞中LIMD1，VEGF-C，CTGF和Survivin的mRNA含量及细胞增殖能力。结果LIMD1的mRNA含量在三组中从小到大顺序为初治组(14.52±1.96)mg，缓解组(76.67±9.56)mg和对照组(111.27±16.45)mg，差异均有统计学意义(P<0.05)；MTT值在三组中从小到大顺序为对照组(100±15.34)，缓解组(151.77±19.34)和初治组(314.45±52.28)，差异均有统计学意义(P<0.05)；VEGF-C的mRNA含量在三组中从小到大顺序为对照组(104.78±15.14)mg，缓解组(146.63±18.84)mg和初治组(259.34±31.45)mg，差异均有统计学意义(P<0.05)；CTGF的mRNA含量在三组中从小到大顺序为对照组(99.27±16.67)mg，缓解组(162.15±19.34)mg和初治组(325.56±36.72)mg，差异均有统计学意义(P<0.05)；Survivin的mRNA含量在三组中从小到大顺序为对照组(109.44±15.78)mg，缓解组(134.39±16.67)mg和初治组(277.74±29.34)mg，差异均有统计学意义(P<0.05)；LIMD1与增殖能力以及VEGF-C，CTGF，Survivin的mRNA含量呈负相关(β=0.679~0.795，P<0.05)。结论LIMD1在急性白血病患者骨髓细胞中的表达减少，且与细胞增殖以及恶性生物学分子VEGF-C，CTGF和Survivin的表达具有相关性。
Abstract:
ObjectiveTo study the abnormal expression of LIM domain-containing protein 1 antibody in acute leukemia patients and its relationship．MethodsFirst diagnosed with acute leukemia patients who did not receive treatment were enrolled in initial treatment group，leukemia patients received chemotherapy and achieve complete remission were enrolled in remission group，non-malignant blood diseases patients with bone marrow normal were enrolled in control group．Then bone marrow cells were isolated and cultured，LIMD1，VEGF-C，CTGF and Survivin mRNA contents and cell proliferation were detected．ResultsFrom the lowest to the highest，the LIMD1 mRNA levels in three groups were initial treatment group (14.52±1.96)mg，remission group (76.67±9.56)mg and control group (111.27±16.45)mg．From the lowest to the highest，the MTT levels in three groups were control group (100±15.34)，remission group (151.77±19.34) and initial treatment group (314.45±52.28)．From the lowest to the highest，the VEGF-C mRNA levels in three groups were control group (104.78±15.14)mg，remission group (146.63±18.84)mg and initial treatment group (259.34±31.45)mg．From the lowest to the highest，the CTGF mRNA levels in three groups were control group (99.27±16.67)mg，remission group (162.15±19.34)mg and initial treatment group (325.56±36.72)mg．From the lowest to the highest，the Survivin mRNA levels in three groups were control group (109.44±15.78)mg，remission group (134.39±16.67)mg，and initial treatment group (277.74±29.34)mg，LIMD1 was negatively correlated with proliferation and mRNA contents of VEGF-C，CTGF，Survivin (β=0.679~0.795，P all<0.05)．ConclusionThe expression LIMD1 in patients with acute leukemia cells decreases and correlates with cell proliferation and the expression of malignant molecular VEGF-C，CTGF，Survivin．

参考文献/References:

［1］Lee JW，Kim HS，Hwang J，et al．Regulation of H-OXA9 activity by predominant expression of DACH1 against C/EBP α and GATA-1 in myeloid leukemia with MLL-AF9［J］．Biochem Biophys Res Commun，2012，426(3)：299-305．
[2]Oram SH，Thoms JA，Pridans C，et al．A previously unrecognized promoter of LMO2 forms part of a transcriptional regulatory circuit mediating LMO2 expression in a subset of T-acute lymphoblastic leukaemia patients［J］．Oncogene，2010，29(43)：5796-5808．
[3]付伟，秦鸿雁，严学倩，等．LIM结构域蛋白FHL1C抑制Notch信号途径的转录激活研究［J］．现代生物医学进展，2011，11(4)：657-661． Fu W，Qin HY，Yan XQ，et al．Inhibitory effects of homo sapiens lim protein FHL1C on notch signaling transcription activities［J］．Progress in Modern Biomedicine，2011，11(4)：657-661．
[4]俞罡，陈静桂．急性髓系白血病患者TLT3-ITD和NPM1基因突变表达及在判断预后中的意义［J］．临床和实验医学杂志，2015，14(7)：565-568． Yu G，Chen JG．Expression of TLT3-ITD and NPM1 gene mutations in patients with acute myeloid leukemia and its significance in the prognosis of patients with acute myeloid leukemia［J］．Journal of Clinical and Experimental Medicine，2015，14(7)：565-568．
[5]Kapoor S．Letter to the editor on “regulation of HO-XA9 activity by predominant expression of DACH1 against C/EBPα and GATA-1 in myeloid leukemia with MLL-AF9”［J］．Biochem Biophys Res Commun，2012，426(4)：664．
[6]Weeks RJ，Kees UR，Song S，et al．Silencing of TE-STIN by dense biallelic promoter methylation is the most common molecular event in childhood acute lymphoblastic leukaemia［J］．Mol Cancer，2010(9)：163-163．
[7]王立敏，李桂玲．LIM同源结构域转录因子LHX-3与LHX-4的研究进展［J］．国际儿科学杂志，2013，40(5)：518-521． Wang LM，Li GL．Advances of the LHX-3 and LHX4 LIM-homeodomain transcription factors［J］．International Journal of Pediatrics，2013，40(5)：518-521．
[8]韩莹．急性白血病患者血清VEGF，IL-8和TF检测的临床意义［J］．现代检验医学杂志，2012，27(4)：132-133． Han Y．Clinical significance of detection of serum VEGF，IL-8 and TF in patients with acute leukemia［J］．Journal of Modern Laboratory Medicine，2012，27(4)：132-133．
[9]陈章兴，戴益琛，谈秋慧．27例大肠癌组织中LIMD1表达特点［J］．胃肠病学和肝病学杂志，2014，23(1)：45-46． Chen ZX，Dai YC，Tan QH．Expression of LIMD1 in 27 cases of colorectal cancer［J］．China Journal of Gastroenterology and Hepatology，2014，23(1)：45-46．
[10]廖子龙，刘琳，王钥，等．LIMD1基因在成人急性白血病患者中的异常表达及临床意义［J］．中国实验血液学杂志，2015，23(1)：34-38． Liao ZL，Liu L，Wang Y，et al．Expression and clinical significance of LIMD-1 gene in adult patients with acute leukemia［J］．Journal of Experimental Hematology，2015，23(1)：34-38．
[11]周琛，环亚红．老年白血病病人Survivin基因mRNA表达及其与预后的关系研究［J］．现代医学，2015，43(2)：190-193． Zhou C，Huan YH．MRNA expression of Survivin gene and its relationship with prognosis in elderly patients with leukemia［J］．Modern Medicine Journal，2015，43(2)：190-193．
[12]刘学文，白学琴，韩轩茂，等．急性白血病治疗前后骨髓中VEGF，CTGF mRNA水平的研究［J］．肿瘤防治研究，2015，42(4)：359-362． Liu XW，Bai XQ，Han XM，et al．VEGF and CTGF mRNA levels in marrow before and after therapy for acute leukemia［J］．Cancer Research on Prevention and Treatment，2015，42(4)：359-362．

相似文献/References:

[1]洪俊,饶永彩.应用ROC曲线确定流式细胞术髓过氧化酶检测结果在急性白血病分类中的最佳临界值[J].现代检验医学杂志,2015,30(05):76.[doi:10.3969/j.issn.1671-7414.2015.05.023]
　HONG Jun,RAO Yong-cai.Detecting Optimal Cut-off Value of Flow Cytometry Myeloperoxidase(fMPO) by Receiver Operator Characteristic Curve in the Classification of Acute Leukemia[J].Journal of Modern Laboratory Medicine,2015,30(06):76.[doi:10.3969/j.issn.1671-7414.2015.05.023]
[2]高 婧,孟伟伟,彭 丽,等.急性白血病患者血清sIL-2R,MCP-1及IFN-γ联合检测的临床意义[J].现代检验医学杂志,2019,34(02):79.[doi:10.3969/j.issn.1671-7414.2019.02.021]
　GAO Jing,MENG Wei-wei,PENG Li,et al.Clinical Significance of Combined Detection of Serum sIL-2R,MCP-1and IFN-gamma in Patients with Acute Leukemia[J].Journal of Modern Laboratory Medicine,2019,34(06):79.[doi:10.3969/j.issn.1671-7414.2019.02.021]

备注/Memo

备注/Memo:
作者简介：杨晓玲(1985-)，女，本科，学士，主管检验师，研究方向：骨髓细胞诊断学，E-mail：hedabao1223@126.com。
更新日期/Last Update: 1900-01-01