[1]商秀娟,张国勇,刘 倩,等.抗烟曲霉果胶裂解酶A抗体检测对侵袭性曲霉病诊断价值的评估[J].现代检验医学杂志,2016,31(01):5-7,11.[doi:10.3969/j.issn.1671-7414.2016.01.002]
　SHANG Xiu-juan,ZHANG Guo-yong,LIU Qian,et al.Evaluation of Diagnostic Value of Antibody Against Aspergillus Fumigatus Pectate Lyase A for Invasive Aspergillosis[J].Journal of Modern Laboratory Medicine,2016,31(01):5-7,11.[doi:10.3969/j.issn.1671-7414.2016.01.002]
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抗烟曲霉果胶裂解酶A抗体检测对侵袭性曲霉病诊断价值的评估()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
5-7,11
栏目:
论著
出版日期:
2016-01-25

文章信息/Info

Title:
Evaluation of Diagnostic Value of Antibody Against Aspergillus Fumigatus Pectate Lyase A for Invasive Aspergillosis
作者:
商秀娟1; 2; 张国勇2; 刘 倩2; 陈 笑2; 李芳秋2
1.江苏大学医学院, 江苏镇江 212013; 2.南京军区南京总医院解放军检验医学研究所中心实验科,南京 210002
Author(s):
SHANG Xiu-juan1; 2; ZHANG Guo-yong2; LIU Qian2; CHEN Xiao2; LI Fang-qiu2
1.School of Medicine of Jiangsu University,Jiangsu Zhenjiang 212013,China; 2.Institute of Medical Laboratory Sciences of PLA,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,China
关键词:
烟曲霉; 果胶裂解酶A; IgG抗体; ELISA
分类号:
R379; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.01.002
文献标志码:
A
摘要:
目的 建立检测抗烟曲霉果胶酶A(pectate lyase A,PlyA)抗体的ELISA方法,评估其对侵袭性曲霉病(invasive aspergillosis,IA)的诊断价值。方法 用重组PlyA作为包被抗原,建立检测人抗PlyA IgG抗体的间接ELISA方法。检测97例IA患者、80例非IA患者和200例体检健康者血清。将所得结果与抗烟曲霉硫氧还蛋白还原酶(thioredoxin reductase,TR)抗体和抗烟曲霉二肽基肽酶V(dipeptidylpeptidase V fragment,DPPV)抗体结果比较。结果 抗PlyA IgG抗体ELISA方法的批内变异系数为5.3%,批间变异系数为10.9%。诊断IA的敏感度为62.9%,特异度为90.4%。非中性粒细胞缺乏IA患者和中性粒细胞缺乏组患者阳性率分别为72.3%和43.8%(χ2=7.493,P< 0.05)。抗PlyA,抗TR,抗DPPV抗体检测的阳性率分别为62.9%,60.8%和66.0%,三者差异无统计学意义(χ2=0.562,P> 0.05); 三种方法联合检测的阳性率可达到92.8%。结论 成功建立了检测抗PlyA IgG的ELISA方法; 抗体检测对非粒细胞减少IA患者的诊断价值均优于粒细胞减少IA患者; 联合三种抗体检测可提高诊断敏感度。
Abstract:
Objective To establish an ELISA for detecting antibody against Aspergillus fumigatus pectate lyase A(anti-PlyA),and evaluate its diagnostic value for invasive aspergillosis(IA).Methods An indirect ELISA for IgG antibody against PlyA was established using PlyA as coating antigen.The serum from 97 IA patients,80 non-IA patients and 200 healthy donors were tested,the results were compared with anti-DPPV(antibody against Aspergillus fumigatus dipeptidyl peptidase V fragment)and anti-TR(antibody against Aspergillus fumigatus thioredoxin reductase).Results The intra-assay coefficients of variation of the ELISA method for detecting anti-PlyA was 5.3%,and inter-assay coefficients of variation was 10.9%.The sensitivity and specificity of diagnosis of IA were 62.9% and 90.4%,respectively.The positive rates of anti-PlyA in non-neutropenic and neutropenic IA patients were 43.8% and 72.3%,respectively(χ2=7.493,P<0.05).There was no significant difference between the positive rates of anti-PlyA(62.9%),anti-TR(60.8%),and anti-DPPV(67.0%)(χ2=0.562,P>0.05).When combined anti-PlyA,anti-TR,and anti-DPPV,the diagnostic sensitivity for IA patients increased to 92.8%.Conclusion An ELISA for detecting anti-PlyA was successfully established.The diagnosticvalue of these three kinds of antibody was superior in non-neutropenic IA patientsto that in neutropenic IA patients.The combined detection of three antibodies could provide higher sensitivity.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金资助项目(81302536)，南京军区医学科技创新重点课题(10Z027)。 作者简介：商秀娟(1990-)，女，硕士研究生，主要从事医学微生物学研究。 通讯作者：李芳秋，Email：njlifq@163com。
更新日期/Last Update: 2016-01-20