[1]吴泽刚,李 艳,郑红云.湖北地区慢性丙型肝炎患者HCV基因分型结果分析[J].现代检验医学杂志,2016,31(01):38-40.[doi:10.3969/j.issn.1671-7414.2016.01.010]
　WU Ze-gang,LI Yan,ZHENG Hong-yun.Analysis on HCV Genotype of Patients with Chronic Hepatitis C inHubei[J].Journal of Modern Laboratory Medicine,2016,31(01):38-40.[doi:10.3969/j.issn.1671-7414.2016.01.010]
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湖北地区慢性丙型肝炎患者HCV基因分型结果分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
38-40
栏目:
论著
出版日期:
2016-01-25

文章信息/Info

Title:
Analysis on HCV Genotype of Patients with Chronic Hepatitis C inHubei
作者:
吴泽刚; 李 艳; 郑红云
武汉大学人民医院检验科,武汉 430060
Author(s):
WU Ze-gang; LI Yan; ZHENG Hong-yun
Department of Clinical Laboratory,Renmin Hospital of Wuhan University,Wuhan 430060,China
关键词:
丙型肝炎病毒;  基因测序;  基因分型
分类号:
R512.63; Q786
DOI:
10.3969/j.issn.1671-7414.2016.01.010
文献标志码:
A
摘要:
目的 应用基因测序技术检测分析湖北地区慢性丙型肝炎患者的HCV基因型及其分布特点。方法 收集2013年2月～2015年7月武汉大学人民医院感染科447例HCV-RNA阳性的慢性丙型肝炎患者血浆标本,通过Sanger测序法测定HCV基因的NS5B区基因序列,然后与NCBI genebank数据库中HCV基因型数据进行比对,分析患者HCV基因型。结果 研究检测的慢性丙型肝炎患者血浆中,共检测出11种HCV基因型,分别是1a,1b,2a,3a,3b,6a,6b,1b/2a混合型,1b/2k混合型,6a/1b混合型和6d/6k混合型。其中,HCV1a型为7例(1.57%),1b型325例(72.71%),2a型67例(14.99%),3a型7例(1.57%),3b型20例(4.47%),6a型14例(3.13%),6b型2例(0.45%),1b/2a混合型2例(0.45%),1b/2k型1例(0.22%),6a/1b混合型1例(0.22%)和6d/6k混合型1例(0.22%)。结论 湖北地区慢性丙型肝炎患者的HCV-RNA基因型以1b型为主,其次为2a型,亦可见其他型别,提示湖北地区HCV流行的基因型呈多样性。
Abstract:
Objective To detect the genotype of hepatitis Cvirus(HCV)in chronic hepatitis C(CHC)infection patients using gene sequence method and observe the distributive characteristic of HCV genotype in Hubei.Methods A total of 447 HCV-RNA-positive plasma samples werecollected from chronic hepatitis C patients in Infectious Diseases Department of Renmin Hospital of Wuhan University from February 2013 to July 2015.Then NS5Bregion gene sequence of HCV genome were detected by Sanger sequencing method and compared with HCV genotype in NCBI genebank database for analyzing HCV genotype.Results A total of 11 kinds of genotypes were detected,including genotypes 1a,1b,2a,3a,3b,6a,6b,1b/2a,1b/2k,6a/1b and 6d/6k,respectively.Detection cases of various genotypes were respectively 7 cases(1.57%),325 cases(72.71%),67 cases(14.99%),7 cases(1.57%),20 cases(4.47%),14 cases(3.13%),2 cases(0.45%),2 cases(0.45%),1 case(0.22%),1 case(0.22%)and 1 case(0.22%).Conclusion Genotype1b was the major type of HCV-RNA genotype,followed by 2a,also other genotypesexisted,which prompted that the prevalence of HCV genotype was diversity in Hubei.

参考文献/References:

[1] Gower E,Estes C,Blach S,et al.Global epidemiology and genotype distribution of the hepatitis C virus infection[J].Journal of Hepatology,2014,61(1 Suppl):S45-S57.
[2] Mohd Hanafiah K,Groeger J,Flaxman AD,et al.Global epidemiology of hepatitis C virus infection:new estimates of age-specific antibody to HCV seroprevalence[J].Hepatology,2013,57(4):1333-1342.
[3] European Association for the Study of the Liver.EASL recommendations ontreatment of hepatitis C 2015[J].Journal of Hepatology,2015,63(1):199-236.
[4] 中华预防医学会医院感染控制分会.中国丙型病毒性肝炎医院感染防控指南[J].传染病信息,2013,26(2):71-75. Nosocomial Infection Control Branch of Chinese Pre.China's guideline on nosocomial infection prevention and control of viral hepatitis C[J].Infectious Disease in Formation,2013,26(2):71-75.
[5] AASLD/IDSA HCV Guidance Panel.Hepatitis C gui-dance:AASLD-IDSA recommendations for testing,managing,and treating adults infected with hepatitis C virus[J].Hepatology,2015,62(3):932-954.
[6] 包安裕,李 艳,童永清,等.湖北地区慢性丙型肝炎患者HCV RNA基因测序分型及疗效评估[J].现代检验医学杂志,2013,28(1):13-16. Bao AY,Li Y,Tong YQ,et al.Gene sequence-based genotyping and therapy evaluation of hepatitis C virus in 168 patients with chronic hepatitis C infection in Hubei province[J].Journal of Modern Laboratory Medicine,2013,28(1):13-16.
[7] Smith DB,Bukh J,Kuiken C,et al.Expanded classification of hepatitis Cvirus into 7 genotypes and 67 subtypes:updated criteria and genotype assignment web resource[J].Hepatology,2014,59(1):318-327.
[8] Messina JP,Humphreys I,Flaxman A,et al.Global distribution and prevalence of hepatitis C virus genotypes[J].Hepatology,2015,61(1):77-87.
[9] Qi Y,Chen Q,Hao F,et al.Subtype distribution of hepatitis C virus in jiangsu,china[J].Journal of Medical Virology,2015,Aug19[Epub ahead of print].
[10] Zhang Z,Yao Y,Wu W,et al.Hepatitis C virus genotype diversityamong intravenous drug users in Yunnan Province,Southwestern China[J].PLoSOne,2013,8(12):e82598.

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备注/Memo

备注/Memo:
基金项目:国家临床重点专科建设项目(财社[2010]305号)。 作者简介:吴泽刚(1985-),男,硕士,医师,研究方向为感染性疾病的分子诊断,E-mail: wuzegang1234@163.com。 通讯作者:李 艳,E-mail: yanlitf1120@163.com。
更新日期/Last Update: 2016-01-20