[1]穆润清,王银铃,颜 青.AST试剂中NADH含量变化对线性范围影响的研究[J].现代检验医学杂志,2016,31(01):121-124.[doi:10.3969/j.issn.1671-7414.2016.01.035]
　MU Run-qing,WANG Yin-ling,YAN Qing.Influence of NADH Concetration in the AST Reagent on Assay Performance[J].Journal of Modern Laboratory Medicine,2016,31(01):121-124.[doi:10.3969/j.issn.1671-7414.2016.01.035]
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AST试剂中NADH含量变化对线性范围影响的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
121-124
栏目:
研究简报·实验技术
出版日期:
2016-01-25

文章信息/Info

Title:
Influence of NADH Concetration in the AST Reagent on Assay Performance
作者:
穆润清; 王银铃; 颜 青
中国医科大学附属第一医院检验科,沈阳 110001
Author(s):
MU Run-qing; WANG Yin-ling; YAN Qing
Department of Clinical Laboratory, the First Affiliated Hospital of China Medical University,Shenyang 110001,China
关键词:
还原型辅酶;  天门冬氨酸氨基转移酶;  线性范围
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2016.01.035
文献标志码:
A
摘要:
目的 评估天门冬氨酸氨基转移酶(AST)试剂中还原型辅酶(NADH)含量变化对试剂盒线性范围的影响。方法 对两个具有不同NADH含量的同一品牌AST试剂的线性范围、精密度、灵敏度、试剂空白等性能指标分别进行验证,利用己糖激酶法测定葡萄糖浓度的方法测定NADH的摩尔消光系数,进而评估NADH浓度变化对分析性能的影响。结果 较高及较低NADH浓度AST试剂的R1试剂中NADH浓度分别为0.21 mmol/L(基础吸光度为1.187 6)和0.13 mmol/L(基础吸光度为0.7567),NADH浓度降低可造成试剂盒的线性范围减小,检测上限由1 629 U/L降至1 263 U/L。NADH浓度变化对两种试剂的精密度、灵敏度、试剂空白等性能指标无明显影响。结论 AST试剂盒的R1试剂中NADH浓度降低(基础吸光度减小)可造成样品检测线性范围减小,因此实验室通过合适的线性评估方案定义其AST试剂能够满足线性范围的基础吸光度标准,对于判断试剂质量、保证高值结果准确是很重要的。
Abstract:
Objective To estimate the assay performance oftwo different kind of reagent with different concentration of NADH.Methods Verificated function index of these two reagents,such as accuracy,sensitiveness,blank space and so on,and determined the molar extinctioncoefficient of NADH using hexokinase method and calculated the NADH density intwo kind of AST reagents.Then estimated the effect of concentation of NADH on the analysis performance.Results NADH concentration was 0.21 mmol/L,and the sample detection limit of the linear was 1 629 U/L.NADH concentration was 0.13 mmol/L,and the sample detection limit of the linear was 1263 U/L.So the gap between the two reagent's detection linear was visible.But there were no significant changes in other performance indicators such as detection sensitivity,reagent blank,precision.Conclusion The NADH concentration in the reagent of AST had greatimpact on the detecting linear range,so should pay attention to the potential change of linear range in the daily work.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:穆润清(1976-),男,博士,主要从事检验项目的参考区间研究,E-mail:runqingmu@163.com。
更新日期/Last Update: 2016-01-20