[1]徐艳杰,艾 亮,谢 欢.Tim-1在儿童过敏性鼻炎患者的表达及临床意义[J].现代检验医学杂志,2016,31(02):46-48,52.[doi:10.3969/j.issn.1671-7414.2016.02.014]
　XU Yan-jie,AI Liang,XIE Huan.Expression of Tim-1 and Its Clinical Significance in Children with Allergic Rhinitis[J].Journal of Modern Laboratory Medicine,2016,31(02):46-48,52.[doi:10.3969/j.issn.1671-7414.2016.02.014]
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Tim-1在儿童过敏性鼻炎患者的表达及临床意义()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年02期
页码:
46-48,52
栏目:
论著
出版日期:
2016-06-01

文章信息/Info

Title:
Expression of Tim-1 and Its Clinical Significance in Children with Allergic Rhinitis
作者:
徐艳杰,艾 亮,谢 欢
郑州市妇幼保健院检验科,郑州 450000
Author(s):
XU Yan-jie,AI Liang,XIE Huan
Department of Clinical Laboratory, Zhengzhou Maternal and Child Health Hospital,Zhengzhou 450000,China
关键词:
Tim-1;  Th2细胞;  儿童过敏性鼻炎;  IgE;  嗜酸性粒细胞
分类号:
R765.21; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.02.014
文献标志码:
A
摘要:
目的 探讨T细胞免疫球蛋白域黏蛋白域蛋白-1(Tim-1)在儿童过敏性鼻炎(allergic rhinitis,AR)中的作用。方法 选择42例AR患儿及同期30例健康儿童作为对照组。Real time qPCR检测AR患儿和对照组外周血单个核细胞(PBMC)Tim-1 mRNA表达水平,流式细胞术检测Th2细胞比例,同时测定两组血清IgE浓度和外周血嗜酸性粒细胞比值。结果 儿童AR患者Tim-1 mRNA水平为0.76±0.16,明显高于对照组0.27±0.08; Th2细胞表达率(20.4%±7.6%),血清IgE浓度(255.78±68.29 IU/ml)明显高于对照组相应检测值(12.3%±6.9%,16.22±6.01 IU/ml),各项检测指标两组之间比较差异均有统计学意义(P<0.05); 且Tim-1 mRNA水平与Th2细胞比例及血清IgE浓度呈显著正相关,而与外周血嗜酸性粒细胞比值间的相关性不明显。结论 AR患儿Tim-1 mRNA高表达,并与Th2及临床指标IgE浓度密切相关,提示Tim-1可能通过调节Th2细胞分化参与儿童AR的发病过程,这为儿童AR临床治疗提供新的方向。
Abstract:
Objective To investigate the mechanism of T cell immunoglobulin domain mucin domain protein -1(Tim-1)in children with allergic rhinitis(AR).Methods 42 children with AR and 30 healthy children were selected.Tim-1 mRNA levels in peripheral blood mononuclearcells(PBMC)were detected by real time qPCR,and Th2 cells were determined by flow cytometry.Serum IgE level and peripheral blood eosinophil ratio were also recorded.Results Tim-1 mRNA in children with AR were significantly increased(0.76±0.16)when compared to compared to control group(0.27±0.08),Th2 cells and serum IgE concentrations were significantly increased(20.4%±7.6%,255.78±68.29 IU/ml)and Tim-1 mRNA levels werepositively correlated with Th2 cell and serum IgE concentrations,and no relationshipwith peripheral eosinophil ratio was observed.Conclusion Tim-1 mRNA was high in AR children and was closely related to IgE and Th2 cells.Thus,Tim-1 may be involved in the pathogenesis of AR children by regulating Th2 cells,providing a new direction for the clinical treatment of AR children.

参考文献/References:

[1] Scadding GK.Optimal management of allergic rhinitis[J].Arch Dis Child,2015,100(6):576-582.
[2] Seidman MD,Gurgel RK,Lin SY,et al.Clinical practice guideline:Allergic rhinitis[J].Otolaryngol Head Neck Surg,2015,152(1 Suppl):S1-43.
[3] Luo Y,Deng Y,Tao Z,et al.Regulatory effect of microRNA-135a on the Th1/Th2 imbalance in a murine model of allergic rhinitis[J].Exp Ther Med,2014,8(4):1105-1110.
[4] Mou Z,Shi J,Tan Y,et al.Association between TIM-1 gene polymorphismsand allergic rhinitis in a Han Chinese population[J].J Investig Allergol Clin Immunol,2010,20(1):3-8.
[5] Curtiss ML, Gorman JV, Businga TR,et al.Tim-1 regulates Th2 responses in an airway hypersensitivity model[J].Eur JImmunol,2012,42(3):651-661.
[6] Lin Z, Zhou L, Luo X,et al.Suppression of TIM-1 predicates clinicalefficacy of sublingual immunotherapy for allergic rhinitis in children[J].IntJ Pediatr Otorhinolaryngol,2013,77(8):1345-1349.
[7] Liu W,Luo R,Chen Y,et al.Interleukin-31 promotes helper T cell type-2 inflammation in children with allergic rhinitis[J].Pediatr Res,2015,77(1):20-28.
[8] 任兴斌,诸葛宝忠,季海生.湿疹患者外周血CD4+CD25+FOXP3+调节性T细胞和血清IL-4,IL-10,TGF-β检测及临床研究[J].现代检验医学杂志,2010,25(3):131-136. Ren XB,Zhuge BZ,Ji HS.Detection of nTreg cell and IL-4,IL-10,TGFq3 in peripheral blood of eczema patients and its clinical significance[J].Journal of Modern Laboratory Medical,2010,25(3):131-136.
[9] Kuchroo VK,Umetsu DT,de Kruyff RH,et al.The TIM gene family:emergingroles in immunity and disease[J].Nat Rev Immunol,2003,3(6):454-462.
[10] de Souza AJ,Oriss TB,O'malley KJ,et al.T cell Ig and mucin 1(TIM-1)is expressed on in vivo-activated T cells and provides a costimulatory signal for T cell activation[J].Proc Natl Acad Sci USA,2005,102(47):17113-17118.

备注/Memo

备注/Memo:
作者简介:徐艳杰(1985-),女,检验技师,研究方向:血液免疫,E-mail:xili39@163.com。
更新日期/Last Update: 2016-02-20