[1]方周宾,林兵英,李卓成,等.AIHA患者外周血Tfh/Treg细胞的检测及临床意义[J].现代检验医学杂志,2016,31(02):70-72.[doi:10.3969/j.issn.1671-7414.2016.02.021]
　FANG Zhou-bin,LIN Bing-ying,LI Zhuo-cheng,et al.Detection of Tfh/Treg Subsets on Peripheral Blood of the Patients with Autoimmune Haemolytic Anaemia[J].Journal of Modern Laboratory Medicine,2016,31(02):70-72.[doi:10.3969/j.issn.1671-7414.2016.02.021]
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AIHA患者外周血Tfh/Treg细胞的检测及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年02期
页码:
70-72
栏目:
论著
出版日期:
2016-06-01

文章信息/Info

Title:
Detection of Tfh/Treg Subsets on Peripheral Blood of the Patients with Autoimmune Haemolytic Anaemia
作者:
方周宾; 林兵英; 李卓成; 伍昌林
深圳市第二人民医院检验科,广东深圳 518035
Author(s):
FANG Zhou-bin; LIN Bing-ying; LI Zhuo-cheng; WU Chang-lin
Department of Clinical Laboratory, the Second People's Hospital of Shenzhen,Guangdong Shenzhen 518035,China
关键词:
自身免疫性溶血性贫血;  Tfh/Treg细胞;  IL-21/IL-10比值
分类号:
R556.62; R392.12
DOI:
10.3969/j.issn.1671-7414.2016.02.021
文献标志码:
A
摘要:
目的 研究自身免疫性溶血性贫血(AIHA)患者外周血CD4+CXCR5+T细胞(Tfh)及CD4+CD25+FoxP3+T细胞(Treg)数量和比例及相关细胞因子的变化,探讨其在AIHA发病机制中的作用。方法 选择30例AIHA患者,分离外周血单核细胞(PBMC),采用流式细胞术(FCM)检测Tfh及Treg细胞的数量,ELISA法测定血清中IL-21,IL-10的水平及IL-21/IL-10比值变化,与同期选择的健康对照组比较。结果 AIHA患者PBMC中Tfh细胞及IL-21因子的水平均高于健康对照组,而Treg细胞及IL-10水平低于对照组,差异均有统计学意义(P<0.05),Tfh/Treg与IL-21/IL-10比值均升高(P<0.05),且IL-21/IL-10比值变化与患者病情呈正相关(P<0.05)。结论 Tfh及Treg细胞的比例异常及相关细胞因子IL-21/IL-10比值变化可能参与AIHA患者的免疫紊乱机制。
Abstract:
Objective To determine the peripheral blood Tfhcell and Treg subsets in patients with Autoimmune Haemolytic Anaemia(AIHA)andexplore their significances.Methods The CD4+CXCR5+T(Tfh),CD4+CD25+FoxP3+T(Treg)subsets of the peripheral blood from 30 patients with AIHA and 30 healthy controls were analyzed by flow cytometry with immunofluorescent staining.The levels of the peripheral blood IL-21 and IL-10 were detected by ELISA assays.Results The numbers of peripheral blood Tfh and the levels of IL-21 were significantly increased in patients with AIHA in comparison with those of healthy controls(P<0.05), but theTreg subsets and IL-10 levels were lower than those of controls.The ratios ofthe Tfh/Treg and the IL-21/IL-10 were higher those of controls,they had significantly difference(P<0.05).Besides,there were positive relation betweenthe ratio of IL-21/IL-10 and the AIHA disease conditions.Conclusion The predominance of Tfh in peripheral blood of AIHA and the lowerTreg levels may play an important role in the pathogenesis of AIHA.

参考文献/References:

[1] Sekiguchi Y,Shimada A,Imai H,et al.CD56+ angioimmunoblastic T-cell lymphoma with evans syndrome:a case report and review of the literature[J].J Clin Exp Hematop,2013,53(1):37-47.
[2] 张之南,沈 悌.血液病诊断及疗效标准[M].2版.北京: 北京科学出版社,1998. Zhang ZN,Shen T.The blood disease diagnosis and curative effect standard[M].2th Ed.Beijing:Beijing Science Press,1998.
[3] Ahearne MJ,Willimott S,Pi(¨overn)on L,et al.Enhancement of CD154/IL4 proliferation by the T follicular helper(Tfh)cytokine,IL-21 and increasednumbers of circulating cells resembling Tfh cells in chronic lymphocytic leukaemia[J].Br J Haematol,2013,162(3):360-370.
[4] Caproni M,Antiga E,Torchia D,et al.FoxP3-expre-ssing T regulatory cells in atopic dermatitis lesions[J].Allergy Asthma Proc,2007,28(5):525-528.
[5] Li Q,Liu Z,Dang E,et al.Follicular helper T cells(Tfh)and IL-21 involvement in the pathogenesis of Bul ous pemphigoid[J].PLoS One,2013,8(7):e68145.
[6] Xie X,Mu L,Yao X,et al.ATRA alters humoral responses associated withamelioration of EAMG symptoms by balancing Tfh/Tfr helper cell profiles[J].Clin Immunol,2013,148(2):162-176.
[7] Visco C,Barcellini W,Maura F,et al.Autoimmune cytopenias in chronic lymphocytic leukemia[J].Am J Hematol,2014,89(11):1055-1062.
[8] Tandra P,Krishnamurthy J,Bhatt VR,et al.Autoimmune cytopenias in chronic lymphocytic leukemia,facts and myths[J].J Hematol Infect Dis,2013,5(1):e2013068.
[9] Goldschmidt N,Gural A,Ben-Yehuda D,et al.Short communication:bendamustine-related hemolytic anemia in chronic lymphocytic leukemia[J].Cancer Chemother Pharmacol,2013,72(3):709-713.
[10] Gravano DM,Hoyer KK.Promotion and prevention of autoimmune disease by CD8+T cells[J].J Autoimmun,2013(45):68-79.
[11] 张 劼,陈军浩.脐血CD4+CD25+CD127low调节T细胞及淋巴细胞亚群分析[J].现代检验医学杂志,2012,27(1):94-96,98. Zhang J,Chen JH.Study on CD4+CD25+CD127 low regulatory T cells and lymphocytes subsets in umbilical cord blood[J].J Mod Lab Med,2012,27(1):94-96,98.

相似文献/References:

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备注/Memo

备注/Memo:
作者简介:方周宾(1982-),男,大学本科,主管技师,主要从事临床检验研究。 通讯作者:伍昌林(1975-),男,主任技师,主要从事血液免疫学研究,E-mail:281320236@qq.com。
更新日期/Last Update: 2016-02-20