[1]秦 琴,王 慧,庄绪冉,等.慢性感染临床微生物样本中持留菌的筛选及分布特点[J].现代检验医学杂志,2016,31(03):1-3.[doi:10.3969/j.issn.1671-7414.2016.03.001]
　QIN Qin,WANG Hui,ZHUANG Xu-ran,et al.Distribution Characteristics of Persisters Isolated from Chronic Infected Patients[J].Journal of Modern Laboratory Medicine,2016,31(03):1-3.[doi:10.3969/j.issn.1671-7414.2016.03.001]
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慢性感染临床微生物样本中持留菌的筛选及分布特点()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
1-3
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Distribution Characteristics of Persisters Isolated from Chronic Infected Patients
文章编号:
1671-7414(2016)03-001-04
作者:
秦 琴; 王 慧; 庄绪冉; 吴林洪; 邓安梅
第二军医大学长海医院实验诊断科,上海 200433
Author(s):
QIN Qin; WANG Hui; ZHUANG Xu-ran; WU Lin-hong; DENG An-mei
Department of Clinical Laboratory Diagnostics, Changhai Hospital of the Second Military Medical University,Shanghai 200433,China
关键词:
慢性感染;  持留菌;  持留菌生长曲线
分类号:
R446.5
DOI:
10.3969/j.issn.1671-7414.2016.03.001
文献标志码:
A
摘要:
目的 了解长海医院慢性感染微生物样本中持留菌的分离及分布情况,为临床采取有效措施预防、控制和治疗持留菌所引起的慢性感染提供合理依据。方法 采用WHONET5.6软件对医院2013年1月～2014年12月收集的微生物样本进行分析,选取阳性分离率最高的4种致病菌,结合临床资料聚焦于慢性感染病例样本,用活菌平板计数法确定菌液浓度,绘制每个临床样本在抗生素作用条件下的生长曲线图,根据曲线特点,筛选出持留菌株,并观察其在临床的分布情况。结果 14 216份微生物样本共筛选得到862株慢性感染病例样本,最终共筛得4株大肠埃希菌,23株肺炎克雷伯菌,8株铜绿假单胞菌,6株金黄色葡萄球菌持留变异株。结论对长海医院临床微生物样本进行回顾性分析,筛选获得41株持留菌,持留菌的临床分离率仍较低,主要来源于痰标本,烧伤科是持留菌的主要分布科室。肺炎克雷伯菌持留变异株分离率相对较高,应注意防控。
Abstract:
Objective To investigate the clinical distribution characteristics of persisters isolated from the chronic infected patients,so as to provide scientific basis for effective clinical measures to prevent,control and treat persister-associated chronic infection.Methods Clinical microbial samples cultured from Jan.2013 to Dec.2014 were analysed by WHONET5.6.Four bacteria species with the highest isolation rate wereperformed for screening of chronic infection.Concentration of bacteria were detected by viable plate count method and then the growth curve were drew of each sample under the presence of antibiotics.Persisters were comfirmed according tothe specific growth curve under the presence of antibiotics.ResultsFour highest isolated bacteria species of the clinical samples wereEscherichia coli(13.2%),Klebsiella pneumoniae(9.6%),Pseudomonasaeruginosa(8%)and Staphylococcus aureus(6.6%).862 chronic infectionsamples were generated out of 14 216 microbial samples and 41 persisters(4 strains of Escherichia coli,23 strains of Klebsiella pneumoniae,8 strainsof Pseudomonas aeruginosa and 6 strains of Staphylococcus aureus)were isolated finally.Conclusion With such a comprehensively retrospective analysis of the hospital clinical microbial samples,can tell thatthe ratio of persisters in chronic infection was not high.And chronic infections are mostly caused by gene-mutated drug-resistant bacteria.However,the isolation rate of Klebsiella pneumoniae was relatively high,of which more attention should be payed to the prevention and control.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81202353),长海医院学科特色培育基金(CH125530300)。 作者简介:秦 琴(1978-),女,硕士,主治医师,研究方向为临床微生物学及细菌耐药机制研究.E-mail:qinq78@163.com。 通讯作者:邓安梅,主任医师,Tel:021-31162061,E-mail:amdeng70@163.com。
更新日期/Last Update: 2016-06-25