[1]朱 涛,赵 涌,彭梦乐,等.特发性膜性肾病患者血清抗PLA2R抗体表达研究[J].现代检验医学杂志,2016,31(03):4-6.[doi:10.3969/j.issn.1671-7414.2016.03.002]
　ZHU Tao,ZHAO Yong,PENG Meng-le,et al.Expression of Serum Anti PLA2R Antibodies in Idiopathic Membranous Nephropathy[J].Journal of Modern Laboratory Medicine,2016,31(03):4-6.[doi:10.3969/j.issn.1671-7414.2016.03.002]
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特发性膜性肾病患者血清抗PLA2R抗体表达研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
4-6
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Expression of Serum Anti PLA2R Antibodies in Idiopathic Membranous Nephropathy
文章编号:
1671-7414(2016)03-004-03
作者:
朱 涛1; 2; 赵 涌1; 彭梦乐1; 施小晴1; 秦东春1
1.郑州大学第一附属医院检验科, 郑州 450052;
2.河南省高等学校临床医学重点学科开放实验室,郑州 450052
Author(s):
ZHU Tao1; 2; ZHAO Yong1; PENG Meng-le1; SHI Xiao-qing1; QIN Dong-chun1
1.Department of Clinical Laboratory,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;
2.Clinical Medicine Key-Disciplines Laboratory,Henan Institution of Higher Learning,Zhengzhou 450052,China
关键词:
特发性膜性肾病;  抗M型磷脂酶A2受体抗体;  IgA肾病;  1型血小板反应蛋白7A域
分类号:
R692; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.03.002
文献标志码:
A
摘要:
目的 分析特发性膜性肾病患者血清抗PLA2R抗体的表达,探讨其在特发性膜性肾病诊断及病情评估中的价值。方法 收集经肾活检确诊的118例肾小球疾病患者,其中包括97例IMN和21例IgA肾病与19例健康体检者作为研究对象,应用ELISA法检测血清抗M型磷脂酶A2受体(PLA2R)抗体表达水平,并分析其与IMN患者血清清蛋白,24 h尿蛋白量等指标的相关性。结果 IMN组,IgA肾病组及健康体检组血清抗PLA2R抗体含量中位数分别为45.2(3.6～705.9)RU/ml,5.9(2.3～10.6)RU/ml和1.2(0.6～9.3)RU/ml,IMN组血清抗PLA2R抗体含量高于IgA肾病组及健康体检组,差异有统计学意义(t=-5.027,-3.077; P均< 0.05); 97例IMN患者中76例血清抗PLA2R抗体阳性,阳性率为78.35%,IgA肾病组与健康体检组均未检出阳性样本。IMN组患者血清抗PLA2R抗体与血清清蛋白呈负相关(r=-0.453,P<0.05); 与肌酐、胆固醇、红细胞沉降率、24 h尿蛋白呈正相关(r=0.233,0.234,0.363,0.586; P均<0.05)。结论 血清抗PLA2R抗体可作为IMN特异性标志物用于IMN的血清学诊断,并对评估IMN患者病情的严重程度有重要参考意义。
Abstract:
Objective To analyze the expression of serum anti M phospholipase A2 receptor(PLA2R)antibody in idiopathic membranous nephropathy(IMN),and to investigate its value in the diagnosis and evaluation of idiopathic membranous nephropathy.Methods One hundred and eighteen patients with biopsy-proved glomerular diseases were involved in this study,including 97 cases with IMN,21 cases with IgA nephropathy(IgAN)and 19 healthy people.ELISA was used to detect serum anti-PLA2R antibodies.Correlations of anti-PLA2R antibody level with laboratory parameters,including serum albumin,24-hour urine protein of IMN patients were evaluated.ResultsThe median of anti PLA2R antibody in IMN group,IgAN group and healthy group was 45.2(3.6～705.9)RU/ml,5.9(2.3～10.6)RU/ml and 1.2(0.6～9.3)RU/ml.The levels of serum anti PLA2R antibody in IMN group were higher than those in IgA nephropathy group and healthy control group.The difference was statistically significant(t=-5.027,-3.077; P=0.05).Among 97 cases with IMN,76 cases showed positive anti-PLA2R antibodies(positive rate 78.35%).There was none patient showed positive anti-PLA2R antibody respectively in IgAN andhealthy people.Furthermore,anti-PLA2R antibody level was negatively correlated with serum albumin(r=-0.453,P=0.000)and positively correlated with CREA,TC,ESR,24 hour urine protein(r=0.233,0.234,0.363,0.586; P=0.004,0.217,0.021,0.000)in IMN patients.Conclusion Serum anti PLA2R antibody may be used as a IMN specific marker for the diagnosisof IMN,and it has important reference value for evaluating the severity of IMN.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:朱 涛(1989-),男,在读硕士,主要从事免疫学检验,Tel:18237013803,E-mail:18237013803@163.com。 通讯作者:秦东春(1963-),男,博士,主任技师,主要从事免疫学检验与检验基础研究,E-mail:qindonchun@163.com。
更新日期/Last Update: 2016-06-25