[1]李 虎a,陈 燕a,张 蕾a,等.原发性胆汁性肝硬化患者 外周血Th9细胞表达增高及其临床意义[J].现代检验医学杂志,2016,31(03):16-18.[doi:10.3969/j.issn.1671-7414.2016.03.005]
　LI Hua,CHEN Yana,ZHANG Leia,et al.Expression of Th9 Cells from Peripheral Blood of Primary Biliary Cirrhosis Patients and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2016,31(03):16-18.[doi:10.3969/j.issn.1671-7414.2016.03.005]
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原发性胆汁性肝硬化患者 外周血Th9细胞表达增高及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
16-18
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Expression of Th9 Cells from Peripheral Blood of Primary Biliary Cirrhosis Patients and Its Clinical Significance
文章编号:
1671-7414(2016)03-016-04
作者:
李 虎a; 陈 燕a; 张 蕾a; 刘挺挺a; 叶 辛b; 郭 杰a; 邓安梅a
第二军医大学长海医院 a.实验诊断科;
b.输血科,上海 200433
Author(s):
LI Hua; CHEN Yana; ZHANG Leia; LIU Ting-tinga; YE Xinb; GUO Jiea; DENG An-meia
a.Department of Laboratory Diagnosis;
b.Department ofTransfusion, Changhai Hospital of the Second Military Medical University,Shanghai 200433,China
关键词:
Th9细胞;  原发性胆汁性肝硬化;  细胞因子
分类号:
R575.22; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.03.005
文献标志码:
A
摘要:
目的 探讨原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者外周血Th9细胞所占百分比并初步探讨其临床意义。方法 应用流式细胞术检测PBC患者(n=38)和健康对照者(n=38)外周血Th9细胞所占百分比,利用临床资料对患者进行Mayo评分。采用实时荧光定量PCR法检测患者组和健康对照组外周血单个核细胞(PBMC)中IL-9,PU-1,TGF-β的表达量。并采用ELISA方法检测患者血清中IL-9的表达量。进一步分析Th9细胞百分比与PBC患者生化指标和Mayo评分的相关性。结果 与健康对照组相比,PBC患者Th9细胞百分比增高(t=27.29; P<0.01),且随疾病分期加重逐渐增高。IL-9,PU-1和TGF-β mRNA的表达量显著增高(t=14.69,23.92,10.48; 均P<0.01),且血清中IL-9的表达量也显著增高(t=11.66; P<0.01)。生化指标(ALP,AST,ALT,GGT,TBIL)较健康对照组均显著增高(t=10.94,12.95,10.56,14.92,27.70; 均P<0.01)。Th9细胞百分比与患者Mayo评分、AST,ALT,GGT和TBIL间呈正相关(P<0.05)。结论 Th9细胞可能参与了PBC发病机制,且与疾病的发展相关,这为未来免疫调节治疗靶点的探索提供了新的依据。
Abstract:
Objective To investigate the role of Th9 cellsin primary biliary cirrhosis and its clinical significance.MethodsThe percentages of Th9 cells in patients with PBC(n=38)and healthy controls(n=38)were measured by flow cytometry.Patients clinical datawere collected and Mayo risk scores were calculated.Determined the levels of IL-9,PU-1 and TGF-βmRNA expressions and the protein levels of IL-9 in plasma.Further analyzed the correlation between Th9 cells and clinical parameters.Results Compared to healthy controls,Th9 cells percentagewas higher in PBC patients and increased with disease stages(t=27.29; P<0.01).IL-9,PU-1 and TGF-βmRNA expressions were increased compared with healthy controls(t=14.69,23.92,10.48; all P<0.01)and the protein level of IL-9 was also up-regulated(t=11.66; P<0.01).The clinical parameters(ALP,AST,ALT,GGT and TBIL)were higher than healthy controls(t=10.94,12.95,10.56,14.92,27.70; all P<0.01).Moreover,the percentageof Th9 cells were positively correlated to Mayo risk,ALT,AST,GGT and TBIL(P<0.05).Conclusion Th9 cells may be involved in thepathogenesis of PBC and related to the disease stage,which provides new cluesfor future immunotherapy.

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81471605,81401358,81501397,81501398),上海申康基金(CHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023),长海医院基金(CH125530300),南京军区科研基金(12MA056)。 作者简介:李 虎(1986-),男,主管技师,大学本科,从事临床检验工作,主要研究方向为感染免疫及自身免疫,Tel:021-31162075,E-mail:lihu1127@126.com。 陈 燕(1977-),女,主管技师,E-mail:chenyan770628@163.com,共同第一作者。 通讯作者:邓安梅,主任医师,教授,E-mail:amdeng70@163.com。
更新日期/Last Update: 2016-06-25