[1]邸 茜,邱 玲,侯立安,等.五种血脂检测系统测定结果的可比性调查[J].现代检验医学杂志,2016,31(03):36-42.[doi:10.3969/j.issn.1671-7414.2016.03.011]
　DI Qian,QIU Ling,HOU Li-an,et al.Investigation on the Consistency of Five Measurement Systems for Serum Lipids[J].Journal of Modern Laboratory Medicine,2016,31(03):36-42.[doi:10.3969/j.issn.1671-7414.2016.03.011]
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五种血脂检测系统测定结果的可比性调查()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
36-42
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Investigation on the Consistency of Five Measurement Systems for Serum Lipids
文章编号:
1671-7414(2016)03-036-08
作者:
邸 茜1; 2; 邱 玲1; 2; 侯立安1; 董军3; 赵 芳1; 李鹏昌1; 王 凯1; 程歆琦1; 国秀芝1
1.中国医学科学院 北京协和医院检验科,北京 100730;
2.中国医学科学院北京协和医学院研究生院,北京 100730;
3.卫生部北京老年医学研究所,北京 100730
Author(s):
DI Qian1; 2; QIU Ling1; 2; HOU Li-an1;  DONG Jun3; ZHAO Fang1; LI Peng-chang1; WANG Kai1; CHENG Xin-qi1; GUO Xiu-zhi1
1.Department of Clinical Laboratory,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing 100730,China;
2.Graduate School ofPeking Union Medical College,Chine Academy to Medical Sciences,Beijing 100730,China;
3.Instit
关键词:
总胆固醇;  三酰甘油;  参考方法
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2016.03.011
文献标志码:
A
摘要:
目的 通过比较5个常用的不同血脂检测系统在使用参考方法赋值新鲜血清校准前后的结果可比性,探讨实现血脂检测一致化的方法。方法采用室内质控总变异(CV%)评价5个血脂检测系统的不精密度。参考美国临床及实验室标准化委员会(CLSI)EP 9A2方案,比对54份新鲜患者血清在5个临床常用的总胆固醇(TC)、三酰甘油(TG)检测系统上的结果,并将不同检测系统结果与均值进行偏倚评估。其中8份样本采用参考方法定值,并评估不同系统的偏倚。尝试采用参考方法赋值的新鲜冰冻血清校准以上检测系统,并使用相同的54份患者新鲜血清再次进行比对及偏倚评估。比对校准前后54份样本在不同检测系统测定的变异。结果 5个检测系统TG总不精密度在3.76%～23.65%之间,TC在2.19%～23.43%之间。各系统结果相关良好,TG和TC的r值分别在0.996 7～0.999 6和0.956 2～0.996 7之间,但系统间结果存在较明显的偏差,各系统最大百分偏差TG在14.72%～34.21%,TC在3.11%～14.57%。用参考方法赋值的血清对不同系统进行校准之后系统间偏差和变异均明显降低。结论用参考方法赋值的新鲜血清校准不同的血脂检测系统,可以有效地提高测定结果的一致性。
Abstract:
Objective To explore achieving the consistent method of blood lipid examination by comparing the results of 5 different blood lipid detection system commonly used in the use of refernce method to assign freach blood serum before and after calibration.Methods Usedthe indoor quality control total variation(CV%)to evaluate the 5 blood lipid examination system of the imprecision.Referenced the United States Clinicaland Laboratory Standardization Institution(CLSI)9A2 EP program,compared with54 fresh blood serum in 5 commonly used examination system of Total Cholesterol(TC)and Triglyceride(TG),and then estimated the bias between the different detection systems and mean value.8 of the samples were determined by the reference method and estimate the bias of different system.The fresh frozen serum samples assigned by reference method were used to evaluate the above examination system,then compare and estimate the bias again with the same 54 fresh serum samples.Compared the variation of 54 samples in different detection system before and after calibration.Results The TG imprecision of 5 examination system were between 3.76%～23.65%,the TC imprecision between 2.19%～23.43%,that mean the results were good,the r value of TG were between 0.996 7～0.999 6 and the TC were 0.956 2～0.996 7.But there were obvious differences between the results of the systems,and the biggest difference were 14.72%～34.21% in TG and 3.11%～14.57% in TC.After use the serum assignment by reference method,the variation of the systems has been significantly decreased.Conclusion Using the reference method to assign the fresh serum of different blood lipid detection system can effectively improve the consistency of the results.

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备注/Memo

备注/Memo:
基金项目:首都卫生发展科研专项项目(首发2016-2-4017)。 作者简介:邸 茜(1978-),女,主管技师,从事临床生化检验,Tel:010-69159704,E-mail:diqian.pumch@qq.com。 通讯作者:邱 玲,Tel:010-69159717,E-mail:lingqiubj@aliyun.com。
更新日期/Last Update: 2016-06-25