[1]王 鑫,张 瑞,李雅静,等.白藜芦醇对金黄色葡萄球菌标准株 抑菌作用及超微结构的影响[J].现代检验医学杂志,2016,31(03):55-58.[doi:10.3969/j.issn.1671-7414.2016.03.015]
 WANG Xin,ZHANG Rui,LI Ya-jing,et al.Antibacterial and Ultrastructure Effect of RES on Staphylococcus Aureus Standard Strains ATCC 25923[J].Journal of Modern Laboratory Medicine,2016,31(03):55-58.[doi:10.3969/j.issn.1671-7414.2016.03.015]
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白藜芦醇对金黄色葡萄球菌标准株 抑菌作用及超微结构的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
55-58
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Antibacterial and Ultrastructure Effect of RES on Staphylococcus Aureus Standard Strains ATCC 25923
文章编号:
1671-7414(2016)03-055-04
作者:
王 鑫张 瑞李雅静董倩倩
河北医科大学第二医院检验科,石家庄 050000
Author(s):
WANG XinZHANG RuiLI Ya-jingDONG Qian-qian
Department of Clinical Laboratory, the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China
关键词:
白藜芦醇 金黄色葡萄球菌 抑菌机制 电镜
分类号:
R378.11; R446.5
DOI:
10.3969/j.issn.1671-7414.2016.03.015
文献标志码:
A
摘要:
目的 研究白藜芦醇(resveratrol,RES)对金黄色葡萄球菌标准株ATCC 25923(简称金葡菌标准株)抑菌作用。方法 采用微量肉汤稀释法,检测金葡菌标准株最低抑菌浓度(minimal inhibitory concentrations,MIC); 通过透射和扫描电子显微镜观察白藜芦醇对金葡菌标准株超微形态的影响。结果 白藜芦醇对金葡菌标准株的MIC为0.256 mg/ml。白藜芦醇对金葡菌标准株作用后,与对照组相比,电镜下可见金葡菌标准株菌体细胞形态变形严重,菌体大小不一,菌体边界粗糙不圆润,细胞壁缺损并有破裂,边缘疏松不光滑,菌体内结构松散,核膜断裂,局部呈现空泡化特征,菌体细胞形态超微结构有明显破坏和抑制作用。结论白藜芦醇对金葡菌标准株有明显抑制和破坏作用,抑制机制可能和破坏细胞膜通透性有关。
Abstract:
Objective To study the antibacterial effect andmechanism of Resveratrol(RES)on Staphylococcus aureus standard strains(ATCC 25923).Methods Using the microdilution protocol,to obtain minimal inhibitory concentrations(MIC)of RES on Staphylococcus aureusstandard strains.Transmission electron microscope(TEM)and scanning electronmicroscope(SEM)were tested to obtain the effect of Staphylococcus aureus on Ultrastructure.Results The MIC of RES to Staphylococcus aureus standard strains ATCC 25923 were 0.256 mg/ml.Compared with control group,transmission electron and scanning electron microscope observation showed that after the act of RES on S.au standard strains,the cell morphologyof S.au standard strains changed serious deformation,cells in all shapes and size,cell boundary roughness,cell wall defected and ruptured,and osteoporosis of edgeo,looseness of its structure,the nuclear membrane rupture,local showed vacuolar characteristics.Cell ultrastructure was obvious damaged and inhibition.Conclusion Rseveratrol had obvious inhibition and destruction to the standard strains of staphylococcus aureus,and the inhibition mechanism may be related to the destruction of cell membrane permeability.

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备注/Memo

备注/Memo:
基金项目:河北省2015年医学科学重点研究课题,编号:20150259。 作者简介:王 鑫(1970-),女,硕士,副主任技师,从事临床检验工作,研究方向:病原微生物和分子生物学,Tel:18233106566,E-mail:wx16136@163.com。
更新日期/Last Update: 2016-06-25