[1]肖 波,毛金娥,陈万新.外周血细胞形态学检查与EBV-DNA定量分析 在小儿传染性单核细胞增多症早期诊断中的价值[J].现代检验医学杂志,2016,31(03):114-116.[doi:10.3969/j.issn.1671-7414.2016.03.032]
　XIAO Bo,MAO Jin-e,CHEN Wan-xin.Value of Detecting Peripheral Blood Cell Morphology Combined with EBV-DNA Quantity in the Initial Diagnosis for Infants Patients with Infectious Mononucleosis[J].Journal of Modern Laboratory Medicine,2016,31(03):114-116.[doi:10.3969/j.issn.1671-7414.2016.03.032]
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外周血细胞形态学检查与EBV-DNA定量分析 在小儿传染性单核细胞增多症早期诊断中的价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
114-116
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Value of Detecting Peripheral Blood Cell Morphology Combined with EBV-DNA Quantity in the Initial Diagnosis for Infants Patients with Infectious Mononucleosis
作者:
肖 波1; 毛金娥1; 陈万新2
1.荆门市第二人民医院病理科,湖北荆门 448000;
2.华中科技大学同济医学院附属协和医院血液病研究所,武汉 430022
Author(s):
XIAO Bo1; MAO Jin-e1; CHEN Wan-xin2
1.Department of Pathology,Jingmen Second People's Hospital,Hubei Jingmen 448000, China;
2.Hematopathy Research Institute,the Affiliated Union Hospital ofTongji Medical College of Huazhong University of Science and Technology,Wuhan 430022,China)
关键词:
实时荧光定量PCR;  EB病毒;  异型淋巴细胞;  外周血细胞形态学检查
分类号:
R512.7; R446.113,Q503
DOI:
10.3969/j.issn.1671-7414.2016.03.032
文献标志码:
A
摘要:
目的 评价外周血异常淋巴细胞(异淋)形态学的检查和实时荧光定量PCR技术检测EB病毒DNA定量在小儿传染性单核细胞增多症早期诊断中的价值。方法 回顾性分析了2013年1月～2014年12月收治的212例传染性单核细胞增多症的患儿,分别抽取其发热初期和1周后抗凝静脉血做外周血涂片细胞形态学检查和实时荧光定量PCR技术检测EBV-DNA定量。结合患儿初诊时的临床表现进行综合分析。异型淋巴细胞>10%及EBV-DNA定量超过1.0×103拷贝为阳性。结果 在212例传染性单核细胞增多症的患儿中,发热初期外周血涂片细胞形态学检查,82例异型淋巴细胞阳性,100例外周血EBV-DNA定量阳性。1周后复检外周血,156例异型淋巴细胞阳性,最高可达56%; 180例EBV-DNA定量阳性。联合检测外周血细胞形态学异型淋巴细胞及EBV-DNA定量,发热初期,阳性125例,较单纯外周血细胞形态学异型淋巴细胞检测(χ2=17.45,P<0.01)及EBV-DNA定量检测(χ2=5.92,P<0.05)均明显提高; 但1周后,阳性190例,较单纯外周血细胞形态学异型淋巴细胞检测明显升高(χ2=18.16,P<0.01),但与EBV-DNA定量检测比较,无明显提高(χ2=2.12,P>0.05)。结论 联合检测外周血细胞学形态及EBV-DNA定量对早期诊断小儿传染性单核细胞增多症十分重要,结合临床表现,能提高诊断的及时性及准确性。
Abstract:
Objective To evaluate the value of detecting abnormal lymphocyte morphology in peripheral blood and Epstein Barr Virus(EBV)DNAquantity through real-time quantitative PCR(RT-qPCR)in the initial diagnosisfor infants patients with infectious mononucleosis(IM).Methods From Jan.2013 to Dec.2014 212 infants patients with IM were analysedretrospectively,which were all in-patients in the hospital.The abnormal lymphocyte morphology in peripheral blood and Epstein Barr Virus(EBV)DNA quantity were both detected in the initial fever period and a week later.The latter was detected by real-time quantitative PCR(RT-qPCR),combined with all the symptoms were all analysed comprehensively.The percentage of abnormal lymphocyte morethan 10% was positive,and the EBV-DNA quantity more than 1.0×103 copy perml was positive,too.Results Of all the infants patients,in the initial fever period,82 patients had more than 10% positive abnormal lymphocyte and 100 patients had positive EBV-DNA quantity.But a week later,156patients had more than 10% positive abnormal lymphocyte,the maximum abnormal lymphocyte was 56%.And 180 patients had positive EBV-DNA quantity.When both abnormal lymphocyte morphology in peripheral blood and Epstein Barr Virus(EBV)DNA quantity were detected,in the initial fever period,125 patients were positive,it rose significantly more than that of abnormal lymphocyte morphology in peripheral blood(χ2=17.45,P<0.01),and that of Epstein Barr Virus(EBV)DNA quantity,too(χ2=5.92,P<0.05).But a week later,190 patients were positive,it rose significantly more than that of abnormal lymphocyte morphology in peripheral blood(χ2=18.16,P<0.01).There was no significantly rising more than that of Epstein Barr Virus(EBV)DNA quantity(χ2=2.12,P>0.05).Conclusion The detecting of peripheral blood cell morphology combined with EBV-DNA quantity are very important in the initial diagnosis for infants patients with infectious mononucleosis.Including allthe symptoms,they could improve the diagnosis timely and accurately.

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备注/Memo

备注/Memo:
作者简介:肖 波(1962-),男,本科,副主任技师,主要从事临床细胞学检验工作。
更新日期/Last Update: 2016-06-25